Re: 먹어도 되는 향(에센셜 오일) 탐구중!!

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Front Med (Lausanne)

. 2024 Feb 16;11:1337785. doi: 10.3389/fmed.2024.1337785

Essential oils: a systematic review on revolutionizing health, nutrition, and omics for optimal well-being

Camila Pezantes-Orellana 1,†,#, Fátima German Bermúdez 2,†,#, Carmen Matías De la Cruz 2, José Luis Montalvo 1, Andrea Orellana-Manzano 2,*

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PMCID: PMC10905622  PMID: 38435393

Abstract

Purpose

Essential oils from various plants have diverse therapeutic properties and are researched extensively. They have applications in medicine, aromatherapy, microbiology, agriculture, livestock, and the food industry, benefiting the population.

Methods

This systematic review followed the PRISMA verification protocol. The study focused on the anti-inflammatory effects, nutraceutical properties, antioxidant and antibacterial activity of essential oils in lemon, orange, cumin, cinnamon, coriander, rosemary, thyme, and parsley. We also looked at their presence in the diet, their effect, their mechanism of action on health, and the most important active compounds. The search was conducted in the PubMed database for the last 12 years of publications, including in vitro, in vivo, and online cell model tests.

Results

Essential oils have been shown to have multiple health benefits, primarily due to their antimicrobial and anti-inflammatory effects. The mechanism of action of cinnamon oil alters bacterial membranes, modifies lipid profiles, and inhibits cell division, giving a potential benefit in protection against colitis. On the other hand, a significant improvement was observed in the diastolic pressure of patients with metabolic syndrome when supplementing them with cumin essential oil. The antimicrobial properties of coriander essential oil, especially its application in seafood like tilapia, demonstrate efficacy in improving health and resistance to bacterial infections. Cumin essential oil treats inflammation. Parsley essential oil is an antioxidant. Orange peel oil is antibacterial, antifungal, antiparasitic, and pro-oxidative. Lemon essential oil affects mouse intestinal microbiota. Thyme essential oil protects the colon against damage and DNA methylation. Carnosic acid in rosemary oil can reduce prostate cancer cell viability by modifying the endoplasmic reticulum function.

Conclusion and discussion

Essential oils have many therapeutic and antiparasitic properties. They are beneficial to human health in many ways. However, to understand their potential benefits, more research is needed regarding essential oils such as coriander, parsley, rosemary, cumin, and thyme. These research gaps are relevant since they restrict understanding of the possible benefits of these crucial oils for health-related contexts.

목적

다양한 식물에서 추출한 에센셜 오일은

다양한 치료 효과를 가지고 있으며,

광범위한 연구가 이루어지고 있습니다.

에센셜 오일은

의학, 아로마테라피, 미생물학, 농업, 축산, 식품 산업 등

다양한 분야에서 활용되어 인류에게 유익을 가져다 줍니다.

방법

이 체계적인 검토는 PRISMA 검증 프로토콜을 따랐습니다.

이 연구는

레몬, 오렌지, 커민, 계피, 고수, 로즈메리, 타임, 파슬리 에센셜 오일의

항염증 효과, 기능성 식품 특성, 항산화 및 항균 작용에 초점을 맞췄습니다.

또한 식단에 포함된 정도, 건강에 미치는 영향, 건강에 미치는 작용 기전, 가장 중요한 활성 화합물을 조사했습니다. 체외, 체내, 온라인 세포 모델 테스트를 포함한 지난 12년간의 출판물을 대상으로 PubMed 데이터베이스에서 검색을 수행했습니다.

결과

에센셜 오일은

주로 항균 및 항염증 효과로 인해

여러 가지 건강상의 이점이 있는 것으로 나타났습니다.

계피 오일의 작용 메커니즘은

세균막을 변화시키고,

지질 프로파일을 수정하며,

세포 분열을 억제하여 대장염 예방에 잠재적인 이점을 제공합니다.

한편,

커민 에센셜 오일을 보충했을 때

대사 증후군 환자의 이완기 혈압이 크게 개선되는 것으로 나타났습니다.

고수 에센셜 오일의 항균성,

특히 틸라피아와 같은 해산물에 적용되는 항균성은

건강 개선과 세균 감염에 대한 저항력 향상에 효과가 있는 것으로 나타났습니다.

커민 에센셜 오일은

염증을 치료합니다.

파슬리 에센셜 오일은 항산화제입니다.

오렌지 껍질 오일은

항균, 항진균, 항기생충, 그리고 산화 촉진제입니다.

레몬 에센셜 오일은

쥐의 장내 미생물 군집에 영향을 미칩니다.

타임 에센셜 오일은

결장의 손상과 DNA 메틸화를 방지합니다.

로즈메리 오일에 함유된 카르노산은

소포체 기능을 조절하여

전립선암 세포의 생존력을 감소시킬 수 있습니다.

Carnosic acid in rosemary oil 

로즈마리(Rosmarinus officinalis [라미아세아과])는 경제적으로나 미각적으로도 유명한 허브로, 많은 나라에서 전통 의학에 사용됩니다. 로즈마리에는 카르노산(CA)과 카르노솔(CS), 즉 아비에탄형 페놀 디테르펜이 함유되어 있는데, 이 성분이 로즈마리의 생물학적, 약리학적 작용의 대부분을 차지하지만, 다른 성분인 로즈마린산도 기여한다는 주장이 제기되고 있습니다. 이 리뷰는 부분적으로 NLRP3 염증성 분해효소의 억제를 통해 알츠하이머병(AD), 파킨슨병(PD), 코로나바이러스 감염증 2019(COVID-19)에 대한 CA와 CS의 잠재적 적용에 초점을 맞추고 있습니다. CA는 NLRP3 활성화의 감소를 유도하는 KEAP1/NRF2 전사 경로의 활성화에 의해 시작되는 2단계 효소 유도를 통해 항산화, 항염증, 신경 보호 효과를 발휘합니다. 또한, 우리는 SARS-CoV-2 감염의 뇌 관련 후유증인 “장기 코로나”에 대한 치료제로서 CA 관련 화합물이 사용될 수 있다고 제안합니다. 코로나19의 원인 중 하나는 폐 상피세포와 혈관 내피세포의 조절되지 않은 염증의 결과로 대식세포에서 발생하는 사이토카인 폭풍입니다. 또한, 불안과 “브레인 포그(brain fog)”와 같은 신경학적 후유증은 팬데믹 기간과 팬데믹 이후의 주요 문제가 되고 있습니다. 많은 보고서에 따르면, 조절되지 않은 NLRP3 염증성 복합체의 활성화가 코로나19의 심각성과 그 여파에 잠재적으로 기여할 수 있다고 합니다. 따라서 NLRP3 염증성 복합체의 활동을 억제하는 것이 급성 폐 질환과 만성 신경학적 후유증 모두에 효과적일 수 있습니다. CA는 전신적으로 작용할 뿐만 아니라 혈액-뇌 장벽을 통과하여 뇌 실질에 도달하여 신경 보호 효과를 발휘하는 것으로 밝혀졌기 때문에, 우리는 CA 또는 CA가 함유된 로즈마리 추출물이 SARS-CoV-2 감염에 의해 유발되는 급성 및 만성 병리학적 사건과 AD 및 PD를 포함한 기타 만성 신경 퇴행성 질환에 대한 효과적인 대응책이 될 수 있다는 증거를 논의합니다.

결론 및 토론

에센셜 오일에는 많은 치료 및 항기생충 특성이 있습니다. 여러 가지 면에서 인체 건강에 유익합니다. 그러나, 고수, 파슬리, 로즈메리, 커민, 타임과 같은 에센셜 오일의 잠재적 이점을 이해하기 위해서는 더 많은 연구가 필요합니다. 이러한 연구 격차는 건강 관련 맥락에서 이러한 중요한 오일의 가능한 이점에 대한 이해를 제한하기 때문에 관련이 있습니다.

Keywords: essential oils, nutrition, omics, bioactive compounds, well-being

1. Introduction

Essential oils (EOs) are fragrant extracts obtained from various plants. Their composition varies depending on the plant species from which they are extracted. It is estimated that more than 200 compounds may be present in these oils. In recent years, essential oils have gained significant popularity in various industries, such as aromatherapy, food flavoring, and natural pharmacological treatments, due to their numerous uses, primary components, and respective properties. Consequently, several applications have been studied, including their antimicrobial, anti-inflammatory, analgesic, and antioxidant properties (1).

The main bioactive compounds of EOs are terpenes and terpenoids, which are responsible for the biological activities mentioned above (2). So, the properties of EOs contribute to the prevention of diseases through different mechanisms of action. In vitro studies are generally carried out, and it has been shown that the anti-inflammatory components of EOs inhibit free radicals that can generate mutations alone. In the DNA. Likewise, when there is prolonged oxidative stress, an excessive accumulation of reactive oxygen species (ROS) can trigger chronic disorders such as metabolic syndrome, cardiovascular diseases, diabetes, and even cancer for the same reason that there are mutations in the DNA. EOs rich in polyphenols and their antioxidant properties act as therapeutic agents for these diseases (3).

Essential oils are used in healthcare to treat specific diseases or health conditions. They can be used to alleviate symptoms associated with conditions such as Alzheimer’s, cardiovascular diseases, sleep/stress disorders, and pain during childbirth (4). Research has shown that rosemary essential oil has potential anticholinesterase inhibitory and antioxidant effects that may help protect the brain from chronic anticholinesterase diseases such as Alzheimer’s. However, more studies are needed to determine the adverse effects or benefits. Additionally, aromatherapy has been found to improve cognitive function in patients with such conditions (5).

Antimicrobials are used for various purposes, including medical use as antiviral agents, immunomodulators, and antibiotics. They are also used as food preservatives due to their antimicrobial and antioxidant properties, which help counteract skin infections and, in addition to preventing food spoilage, are used to combat microorganisms that could be transmitted through food (4). According to Swamy et al. (6), lemon has antiviral properties against the influenza virus, while cinnamon is effective against enterobacteria.

Essential oils can affect bacteria differently, depending on their chemical components. Some oils can kill the bacteria (bactericidal action), while others can only slow their growth (bacteriostatic action). Essential oils can also affect cellular processes, such as nutrient processing, molecule synthesis, and regulation of biological processes between cells. Many plants with these characteristics are still being studied, and more findings are expected as they are widely used. These plants include cinnamon, thyme, rosemary, lemon, orange, cumin, and parsley (4). This systematic review identifies literature from the past 10 years to highlight their uses and effects on health, diet, microbiota, and mechanism of action.

1. 소개

에센셜 오일(EO)은

다양한 식물에서 추출한 향기로운 추출물입니다.

에센셜 오일의 성분은

추출한 식물의 종류에 따라 다릅니다.

에센셜 오일에는

200가지 이상의 화합물이 함유되어 있을 것으로 추정됩니다.

최근 몇 년 동안 에센셜 오일은 다양한 용도, 주요 성분, 그리고 각각의 특성 때문에 아로마테라피, 식품 향료, 자연 약리 치료 등 다양한 산업 분야에서 큰 인기를 얻고 있습니다. 결과적으로 항균, 항염, 진통, 항산화 특성을 포함한 여러 가지 응용이 연구되었습니다(1).

에센셜 오일의 주요 생체 활성 화합물은

앞서 언급한 생물학적 작용을 담당하는

테르펜과 테르페노이드입니다(2).

따라서

에센셜 오일의 특성은 다양한 작용 기전을 통해 질병 예방에 기여합니다.

일반적으로 체외 연구가 수행되고 있으며, 에센셜 오일의 항염 성분이 돌연변이를 유발할 수 있는 활성 산소를 억제하는 것으로 나타났습니다. DNA에서. 마찬가지로, 장기간 산화 스트레스가 지속되면 활성산소(ROS)가 과도하게 축적되어 DNA에 돌연변이가 발생하는 것과 같은 이유로 대사증후군, 심혈관 질환, 당뇨병, 심지어 암과 같은 만성 질환을 유발할 수 있습니다. 폴리페놀이 풍부하고 항산화 특성이 있는 EO는 이러한 질병에 대한 치료제로 작용합니다(3).

에센셜 오일은 특정 질병이나 건강 상태를 치료하기 위해 의료 분야에서 사용됩니다. 알츠하이머, 심혈관 질환, 수면/스트레스 장애, 출산 중 통증과 같은 증상을 완화하는 데 사용할 수 있습니다(4). 연구에 따르면 로즈마리 에센셜 오일은 잠재적인 항콜린에스테라제 억제 및 항산화 효과가 있어 알츠하이머와 같은 만성 항콜린에스테라제 질환으로부터 뇌를 보호하는 데 도움이 될 수 있습니다. 그러나 부작용이나 이점을 확인하기 위해서는 더 많은 연구가 필요합니다. 또한, 아로마테라피는 이러한 질환을 가진 환자의 인지 기능을 향상시키는 것으로 밝혀졌습니다(5).

항균제는 항바이러스제, 면역 조절제, 항생제로 의료용을 포함하여 다양한 목적으로 사용됩니다. 또한, 항균 및 항산화 특성으로 인해 식품 방부제로도 사용되는데, 이는 피부 감염을 막는 데 도움이 되며, 식품 부패를 방지하는 것 외에도 식품을 통해 전염될 수 있는 미생물을 퇴치하는 데 사용됩니다(4). Swamy 외(6)에 따르면, 레몬은 인플루엔자 바이러스에 대한 항바이러스 성질을 가지고 있으며, 계피는 장내 세균에 효과적입니다.

에센셜 오일은 화학 성분에 따라 박테리아에 다른 영향을 미칠 수 있습니다. 어떤 오일은 박테리아를 죽일 수 있고(살균 작용), 어떤 오일은 박테리아의 성장을 늦출 수 있습니다(정균 작용). 에센셜 오일은 영양소 처리, 분자 합성, 세포 간의 생물학적 과정 조절과 같은 세포 과정에도 영향을 미칠 수 있습니다. 이러한 특성을 가진 많은 식물이 여전히 연구되고 있으며, 널리 사용되고 있기 때문에 더 많은 연구 결과가 나올 것으로 예상됩니다.

이러한 식물에는

계피, 타임, 로즈마리, 레몬, 오렌지, 커민, 파슬리 등이 포함됩니다(4).

이 체계적인 검토는

지난 10년 동안의 문헌을 통해 건강, 식단, 미생물군, 작용 메커니즘에 대한 용도와 효과를 강조합니다.

2. Methods

This systematic review follows the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) verification protocol (7) with keywords in the search strategy as Essential oils, Nutrition, Omics, Bioactive Compounds, Well-being, properties, Application in Foods previously published experimental and/or clinical case trials in mouse models and/or cell lines and studies in human participants were used along with the use of search operators AND, OR. The study focuses on the anti-inflammatory effects, nutraceutical properties, antioxidant, and antibacterial activity, presence in food, effect and mechanism of action on health, and the most important active compounds of essential oils such as lemon, orange, cumin, cinnamon, coriander, rosemary, thyme, and parsley by searching for articles conducted in a cell line (in vitro), mouse model, in vivo specifically in PubMed database.

The study selection process was conducted in two stages. In the first stage, three authors independently reviewed the titles and abstracts of the studies based on the inclusion and exclusion criteria. They identified the studies that met the inclusion criteria and excluded the ones that did not. In the second stage, two authors thoroughly screened all the full articles and excluded the studies that did not meet the inclusion criteria. We developed custom search strategies for the PubMed bibliographic database. The search was conducted directly and included all articles published within the last 12 years without language restrictions. Additionally, we thoroughly examined the reference lists of the selected articles to identify any relevant research that may have been missed during the electronic database search. Three authors gathered the required data from the reports that had been selected. For all incorporated studies, the following details were recorded: author(s), year of publication, type of essential oil, its impact on health, its effects on health, and its presence in the diet. The authors independently reviewed all full articles.

3. Eligibility criteria

3.1. Inclusion and exclusion criteria

Studies were considered eligible if they met the following criteria: (1) retrospective experimental and clinical studies in human subjects and mouse models and/or cell lines, (2) studies investigating the health benefits of the essential oils described above, (3) studies with quantitative and qualitative data, (4) studies without any language restrictions, (5) systematic reviews, and (6) manuscripts in journals with an impact index.

The following studies were excluded: (1) not meeting the objectives of the article, (2) university theses, (3) not meeting the search criteria, (4) book chapters or books, (5) review articles published more than 12 years ago, and (6) studies not found in the database described above.

3.2. Search strategies

To refine the search, specific keywords such as “Essential oils” “Nutrition” “Omics” “Bioactive compounds” “Food application “Wellness” “properties” “mouse models ““experimental” “clinical” “cell lines” were used together with the use of search operators (Nutrition* OR “dietary” OR “nutritional* content” OR “macronutrients” OR “macronutrients” OR “diet”) AND (“Essential oils*” OR “volatile oils* “OR “Extracted oils* OR “Aromatherapy oils*” OR “Plant oils* “) AND (“Essential oils*” OR “Plant oils*” OR “macronutrients” OR “diet”) AND (“Essential oils*” OR “volatile oils* “OR “Extracted oils* Aromatherapy oils*” OR “Plant oils*) AND (Omics* OR “Microbiota*” OR “Genomics*” OR” Metabolomics*”) AND (Bioactive compounds* OR “Functional ingredient” OR “Major active compound *” OR “Active substance” OR “Health-promoting compound*”) AND (Food application * OR “Application in Food Safety “OR “Application in Food Industry”) AND (Wellness* OR “health “) AND (properties* OR “Aspects “OR “Traits”) performed in electronic databases such as Pubmed, ScienceDirect and Scopus.

4. Results

4.1. Study selection

In the first phase of study selection, 107 citations were found in the PubMed electronic database. After thoroughly reviewing the abstracts, we excluded 27 articles with 12 duplicates. We then identified 85 additional articles through PubMed and reviewed their full text. Thus, this study included 80 references (Figure 1).

Figure 1.

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Flow diagram of literature search and selection criteria for essentials oils from PRISMA.

4.2. Study characteristics

The table below summarizes the key features of the studies analyzed in this research. These studies were conducted in different countries and published between 2011 and 2023 in both English and Spanish. It was observed that the number of publications increased significantly from 2019. All the selected studies were focused on essential oils, with most of them investigating the properties of these oils, such as their antimicrobial and anti-inflammatory effects. The studies have produced significant findings on the benefits of using essential oils, including inhibiting pathogenic microorganisms, preventing radical formation, acting as natural antioxidants and antimicrobial agents, exhibiting anticancer effects in the intestinal microbiota, and preserving food naturally.

Table 1 shows the type of food used, the analysis of the major active constituent identified, and relevant results in the essential oils performed in vivo or in vitro. The main groups of constituents were in cinnamon essential oil cinnamaldehyde, coriander essential oil β-linalool, camphor, geranyl acetate, and cymene; in cumin essential oil cumin aldehyde, and parsley essential oil myristicin, apiole, α-pinene, and β-pinene. Table 2 shows the most predominant bioactive compounds, their health effects, and tentative mechanisms. The main compounds in lemon and orange essential oils are limonene. In cinnamon essential oil, cinnamaldehyde; in Cilantro essential oil, linalool; in Parsley essential oil, myristicin; in Rosemary essential oil monoterpenes; in Thyme essential oil, thymol with antioxidant activity, antimicrobial, inflammatory activity, and innate immune responses.

Table 1.

Essential oils and their used as food.

Essential oilFood use typeType of study / cell line model (in vitro), mouse model, in vivoStudy nameRelevant resultsMajor active compoundReference

1	Cinnamon	This study was carried out to eval‎uate the effects of cinnamon (CO), rosemary (RO) and a mixture (MO) of these plants oil on egg production, feed intake, feed conversion rate, external and internal egg quality, blood serum mineral contents and hatchability traits in different sex.	in vivo	Effects of cinnamon and rosemary oils on egg production, egg quality, hatchability traits and blood serum mineral contents in laying quails (Coturnix coturnix Japonica).	The addition of cinnamon oil to the diets significantly reduced Feed conversion ratio (FCR).	Cinnamaldehyde (88.2%)	(8)
2	Coriander	Essential Oil for Its Application in Foods	in vitro	Antioxidant, antimicrobial and antibiofilm activity of coriander (Coriandrum sativum L.) essential oil for its application in foods	The major volatile compounds of the coriander essential oil in the present study were β-linalool 66.07%. Coriander essential oil radical scavenging activity was 51.05% inhibited. Coriander essential oil expressed the strongest antibacterial activity against B. subtilis followed by S. maltophilia and Penicillium expansum. The strongest antibiofilm activity of the coriander essential oil was found against S. maltophilia.	β-linalool 66,07%, alcanfor 8,34%, acetato de geranilo 6,91% y cimeno 6,35%	(9)
3	Coriander	Inclusion of coriander oil in tilapia feed to improve the health of tilapia and its resistance to bacterial infections	in vivo	Dietary coriander (Coriandrum sativum L) oil improves antioxidant and anti-inflammatory activity, innate immune responses, and resistance to Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus)	Coriander oil with 1% incorporation in feed improves tilapia health and resistance to bacterial infection.	Inalool and geranyl acetate	(10)
4	Cumin	Cumin volatile oils suppressed the activation of neutrophils and might show therapeutic potential for the treatment of neutrophilic inflammatory diseases.	In vitro	Anti-Inflammatory and Antimicrobial Volatile Oils: Fennel and Cumin Inhibit Neutrophilic Inflammation via Regulating Calcium and MAPKs	Suppressed the activation of human neutrophils, including respiratory burst and the degranulation induced by formyl peptide receptor agonists fMLF/CB and MMK1 in the human neutrophils (IC50, 3.8–17.2 μg/mL).	Cumin volatile oil (CUMIN) revealed high content of cuminaldehyde (49.9%)	(11)
5	Cumin	The supplementation with cuminum essential oils decreased diastolic blood pressure (BDP) in patients with metabolic syndrome	Randomized and controlled clinical trial	Effects of cumin (Cuminum cyminum L.) essential oil supplementation on metabolic syndrome components: A randomized, triple-blind, placebo-controlled clinical trial	56 patients with MetS aged 18–60 years received either 75 mg CuEO or placebo soft gel thrice daily for 8 weeks. DBP was significantly lower in CuEO compared with the placebo group at the end of study (81.41 ± 5.88 vs. 84.09 ± 5.54 mmHg, MD with 95% CI: −3.98 [−7.60, −0.35] mmHg, p < 0.05).	Not mentioned	(12)
6	Parsley	Parsley presented the best antioxidant profile	In vitro	Chemical Composition, Antioxidant and Antimicrobial Activity of Essential Oils from Organic Fennel, Parsley, and Lavender from Spain	Parsley had the highest phenolic content. Overall, parsley presented the best antioxidant profile, given its highest % of inhibition of DPPH radical (64.28%) and FRAP (0.93 mmol/L Trolox), but had a pro-oxidative behavior by TBARS.	Myristicin (36.15%), apiole (20.97%), α-pinene (15.47%), and β-pinene (10.43%).	(13)

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Table 2.

Actions in the health of the essential oils.

Essential oilHealth actionType of studyStudy nameRelevant resultsMajor active compoundTentative mechanism (how this essential oil has the effect on health)Reference

1	Lemon	Showed DPPH radical scavenging activity Demonstrated a good inhibitory activity against the pathogenic microorganisms C. albicans, L. monocytogenes and S. aureus.	Cell line model	Waste Citrus limon Leaves as Source of Essential Oil Rich in Limonene and Citral: Chemical Characterization, Antimicrobial and Antioxidant Properties, and Effects on Cancer Cell Viability	It showed DPPH radical scavenging activity (IC50 value = 10.24 mg/mL) and demonstrated a good inhibitory activity against the pathogenic microorganisms C. albicans, L. monocytogenes and S. aureus. Moreover, the treatment with LLEO significantly affected cell viability and morphology from 25 μM in cancer HeLa and A375 cells, with lower effects on normal fibroblasts and keratinocytes.	Limonene (256.7 ± 2.4 mg/mL), Citral (194.5 mg/mL)	The antimicrobial activity against bacterial species tested were strain dependent. The antioxidant activity is given as IC50 value, which indicates the LLEO concentration required to give a 50% inhibition of the DPPH• radical formation LLEO exhibited good antioxidant activity, with an IC50 value of 10.24 ± 2.8 mg/mL.	(14)
2	Lemon	Antioxidant and antimicrobial activities with its preservative effect against Listeria monocytogenes	Inoculated in minced beef meat	Citrus lemon essential oil: chemical composition, antioxidant, and antimicrobial activities with its preservative effect against Listeria monocytogenes inoculated in minced beef meat	The application of ClEO at a 0.06 and 0.312 mg/g, may open new promising opportunities for the prevention of contamination from and growth of pathogenic bacteria, particularly L. monocytogenes, during minced beef meat storage at 4°C. Additionally, during storage period, physicochemical values were higher in control meat than treated meat with ClEO suggesting an efficient antioxidant activity of ClEO.	Limonene (39.74%) and β-Pinene (25.44%).	It is demonstrated, in vitro and in situ, the efficiency of ClEO as a natural antioxidant and antimicrobial agent.	(15)
3	Lemon/ Grapefruit	Antibacterial activity and antioxidant activity	In vitro	The Chemical Composition and Antibacterial and Antioxidant Activities of Five Citrus Essential Oils	The LEO and DLEO demonstrated greater antioxidant activity, measured as DPPH and ABTS free radicals compared with other EOs. The high concentration of d-limonene appeared not to be necessarily associated with high antibacterial and antioxidant activity, suggesting that the antibacterial and antioxidant activities might be related to the active component profiles and possibly their synergism effects.	Monoterpenes (d-limonene and less so sesquiterpenes)	The antibacterial activities of the EOs on three bacteria (Escherichia coli, Salmonella and Lactobacillus acidophilus) were tested by measuring the minimum inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and inhibition zone diameter (IZD).	(16)
4	Orange	The EOP and EOPD showed antioxidant activity by reducing metals, and particularly the EOP by also neutralizing free radicals. They partially affected the bacterial growth while strongly inhibiting the biofilm formation and viability of sessile bacteria living in a pre-existent biofilm (Gram-negative and Gram-positive)	In vitro	Citrus sinensis Essential Oils an Innovative Antioxidant and Antipathogenic Dual Strategy in Food Preservation against Spoliage Bacteria	They could represent natural and safe alternatives to extend the shelf life of food products by preventing oxidation and contamination by pathogens that spoil food, meaning the sweet orange EOs can be considered as an innovative dual strategy for food preservation.	The main monoterpene was limonene (90.41%), myrcene (3.19%)	Could represent natural and safe alternatives to extend the shelf life of food products by preventing oxidation and contamination by food-spoiling pathogens, meaning that sweet orange EOs can be considered as an innovative dual strategy for food preservation.	(17)
5	Orange	Phytoactive chemicals found in Citrus oil can serve as prototypes in the production of new antimicrobial and antiparasitic drugs.	In vitro	Citrus sinensis Peel Oil Extraction and Eval‎uation as an Antibacterial and Antifungal Agent	The antibacterial potential of oil against bacterial strains, i.e., E. coli, S. aureus and S. agalactiae exhibited that the oil efficiently controls the bacterial growth. The highest zone of inhibition was observed against the strain E. coli. The antifungal potential of oil for pathogenic fungal strains, i.e., A. flavus, A. niger, A. alternata showed that the oil significantly inhibits fungal growth.	β-pinene (0.55%), limonene (96–98%), α-pinene (0.29%), myrcene (1.3–1.45%) and octanol (0.37–0.53%).	The antibacterial potential of the oil against bacterial strains, i.e., E. coli, S. aureus and S. agalactiae, demonstrated that the oil efficiently controls bacterial growth. The diameter of the inhibition zone was maximum against A. flavus. Phytoactive chemicals found in Citrus oil can serve as prototypes in the production of new antimicrobial and antiparasitic drugs.	(18)
6	Cinnamon	Significant decrease in fasting blood glucose, plasma C-peptide, serum triglyceride, total cholesterol, and blood urea nitrogen levels, with significant increase in high-density lipoprotein after 35 days. Glucose tolerance was improved and pancreatic islet β-cells showed increased immunoreactivity.	In vivo (Rat), STZ	Encapsulation of cinnamon oil in whey protein counteracts the disturbances in biochemical parameters, gene expression‎, and histological picture of the liver and pancreas of diabetic rats	Both doses improved glucose, insulin, SOD, GSH, amylase, lipid profile and hepatic MDA levels. Gene expression‎ was modulated to favor antidiabetic results. Positive histological changes are observed in the liver and pancreas.	More than 86% of these volatiles: cinnamaldehyde (88.7%), 1,8 cineole (2.02%), acetic acid, 1,7,7 trimethylbicyclo [2.2.1] hept2yl ester (1.79%), α-Pinene (1.45%), and α-Terpineol (0.92%)	The protective role of COE against disturbance in biochemical parameters, antioxidant capacity, gene expression‎, and histological changes in the liver and pancreas in STZ-treated rats was eval‎uated. It was reported that STZ has a rapid action on pancreatic β cells and induces a massive reduction of these cells in islets of Langerhans resulting in hyperglycemia and the ROS generation in β cells following the increasing of ROS in other organs	(19)
7	Cinnamon	Cinnamomum zeylanicum Blume offers novel approach to chemotherapy treatment	in vitro	Optimization of Cinnamon (Cinnamomum zeylanicum Blume) Essential Oil Extraction: Eval‎uation of Antioxidant and Antiproliferative Effects	Cinnamon essential oil was able to remove hydrogen peroxide depending on the amount, 0.7 mg/mL of cinnamon essential oil exhibited 50% hydrogen peroxide removing activity.	cinnamaldehyde (77.34%), transcinnamyl acetate (4.98%), benzenedicarboxylic acid (3.55%), α-pinene (2.6%) and coumaric acid (1.79%)	Essential oils seem to be useful as possible antitumor agents. This work studied the effect of different concentrations of Cinnamomum zeylanicum Blume essential oil against HeLa (0.0625 to 4 μ g/mL) and Raji (0.375 to 12 μ g/mL) cell lines. mL	(20)
8	Coriander	The coriander oil and linalool combinations conferred a synergistic anthelmintic effect	In vitro	Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro	The coriander oil and linalool showed the most inhibitory effects against L3s. Combined treatment using coriander oil enhanced with additional linalool offered more synergistic effect on larval motility indicated by the extensive cuticular damage of treated L3s.	linalool (68.03%), α-tinene (9.71%), γ-terpinene (10.48%) and camphor (11.76%)	The mode of action of coriander oil and linalool is related to disruption of membrane function	(21)
9	Coriander	Inhibition of 65 essential oils and 21 essential oil mixtures against several species/clinical strains of dermatophytes of two main genera, Microsporum and Trichophyton	In vitro	Activity of various essential oils against clinical dermatophytes of Microsporum and Trichophyton	All genera/species tested were completely inhibited for 21 days after a single application	The exact content of the individual components of each oil was not tested or eval‎uated	Inhibition of strain growth for a long time	(22)
10	Parsley	Parsley presented the best antioxidant profile	In vitro	Chemical Composition, Antioxidant and Antimicrobial Activity of Essential Oils from Organic Fennel, Parsley, and Lavender from Spain	Parsley had the highest phenolic content. Overall, parsley presented the best antioxidant profile, given its highest % of inhibition of DPPH radical (64.28%) and FRAP (0.93 mmol/L Trolox), but had a pro-oxidative behavior by TBARS.	myristicin (36.15%), apiole (20.97%), α-pinene (15.47%), and β-pinene (10.43%).	The antioxidant activity of organic fennel, parsley, and lavender EOs was assessed by eval‎uating hydrogen donating ability, or radical scavenging activity, using the stable radical DPPH.	(13)
11	Rosemary	Eval‎uation of Their Chemical Composition, Genotoxicity, Antimicrobial, Antiviral, and Antioxidant Properties	In vitro	Rosemary Extract and Essential Oil as Drink Ingredients: An Eval‎uation of Their Chemical Composition, Genotoxicity, Antimicrobial, Antiviral, and Antioxidant Properties	The essential oil in dilutions of up to 5% and the extract in the range of 25–90% are not genotoxic, and confer antiviral, antifungal, and antioxidant properties; the extract also confers important antibacterial properties, whereas the essential oil is mainly effective against S. aureus.	Monoterpenes (95.57%), sesquiterpenes amounted only to 4.24%	The antioxidant activity was eval‎uated by the DPPH radical method, the Ferric reducing antioxidant power (FRAP) assay and the ABTS assay and the radical scavenging activity among rosemary essential oils is mainly due to the different amounts of major compounds in the essential oils	(23)
12	Thyme	To determine the bioactive compounds, present in thyme essential oil and utilize the nanoemulsion technique to enhance its protective capacity against oxidative stress, genotoxicity, and DNA damage caused by biosynthesized titanium dioxide nanoparticles.	in vivo	Improvement of the antioxidant activity of thyme essential oil against biosynthesized titanium dioxide nanoparticles-induced oxidative stress, DNA damage, and disturbances in gene expression‎ in vivo	The TEO nanoemulsion enhances the biological activity of the oil, improves its antioxidant properties, and provides protection against oxidative damage and genotoxicity induced by TiO2-NP.	The successful identification of 17 bioactive compounds was achieved in thyme essential oil (TEO), with thymol and carvacrol being the main components present in this essential oil.	Sixty male Sprague–Dawley rats were divided into six groups and orally treated for a period of 21 days. These groups included the control group, the TEO-treated group, the TEON-treated groups, the TiO2-NP-treated group, and those groups that received both TiO2-NP and TEO. Blood and tissue samples were collected for various analyses.	(24)

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Table 2 shows how this essential oil affects health, the analysis of the major active constituent identified, and relevant results in the essential oils performed. The lemon essential oil has been found to contain a significant amount of limonene, which has strong antioxidant properties and can help eliminate DPPH radicals (16). Research has shown that it can also be effective as a food preservative, with concentrations of 0.06 and 0.312 mg/g potentially preventing the growth of pathogenic bacteria like L. monocytogenes (15). Limonene may also positively affect the immune system and intestinal microbiota (25). On the other hand, orange essential oil is also a natural citrus preservative in food due to its antimicrobial properties. Its main compounds, citral, and linalool, have been found to have superior effects on intestinal microbiota in mice when compared to lemon essential oil (17). It is important to note that further research is needed to fully understand the potential benefits of these essential oils in the areas mentioned. And findings regarding essential oils like parsley, cumin, coriander, thyme, and rosemary were relatively scarce, mostly showing effects on health. Mainly, parsley exhibits both antioxidant and prooxidative behavior, making it a promising subject for further investigation. Cumin, with volatile compounds inhibiting neutrophil activation, is strongly associated with treating inflammatory pathologies. Supplementation with cumin in patients with metabolic syndrome is estimated to decrease diastolic pressure (12). Coriander is known for its resistance to bacterial infections and tends to improve health in tilapia (10). Thyme demonstrated inhibition of cell proliferation, varying with the dose in all eval‎uated tumor cell lines (26).

Essential oils: a systematic review on revolutionizing health, nutrition, and omics for optimal well-being - PMC

표 2는 이 에센셜 오일이 건강에 미치는 영향, 확인된 주요 활성 성분의 분석, 그리고 수행된 에센셜 오일 관련 결과를 보여줍니다. 레몬 에센셜 오일에는 강력한 항산화 특성을 가지고 있으며, DPPH 라디칼을 제거하는 데 도움이 되는 리모넨이 상당량 함유되어 있는 것으로 밝혀졌습니다(16). 연구에 따르면, 0.06~0.312mg/g의 농도로 레몬 에센셜 오일을 식품 방부제로 사용하면 L. monocytogenes와 같은 병원성 박테리아의 성장을 잠재적으로 방지할 수 있는 것으로 나타났습니다(15).

리모넨은 또한 면역 체계와 장내 미생물 군집에 긍정적인 영향을 미칠 수 있습니다(25). 한편, 오렌지 에센셜 오일은 항균 특성 때문에 식품의 천연 감귤류 보존제이기도 합니다. 이 오일의 주성분인 시트랄과 리날로올은 레몬 에센셜 오일에 비해 생쥐의 장내 미생물 군집에 더 큰 영향을 미치는 것으로 밝혀졌습니다(17). 앞서 언급한 분야에서 이 에센셜 오일이 가진 잠재적 이점을 완전히 이해하기 위해서는 추가 연구가 필요하다는 점을 유의해야 합니다.

그리고 파슬리, 커민, 고수, 타임, 로즈메리와 같은 에센셜 오일에 대한 연구 결과는 상대적으로 부족했으며, 대부분 건강에 미치는 영향에 관한 것이었습니다. 주로 파슬리는 항산화 및 산화 촉진 작용을 모두 나타내므로 추가 연구가 필요한 유망한 주제입니다. 커민은 호중구 활성화를 억제하는 휘발성 화합물을 함유하고 있어 염증성 질환 치료에 효과적입니다. 대사증후군 환자의 커민 보충은 이완기 혈압을 감소시키는 것으로 추정됩니다(12). 고수풀은 박테리아 감염에 대한 저항성이 있는 것으로 알려져 있으며, 틸라피아의 건강을 개선하는 경향이 있습니다(10). 타임은 모든 평가된 종양 세포주에서 투여량에 따라 세포 증식을 억제하는 것으로 나타났습니다(26).

Table 3 highlights the various omics mechanisms of the essential oils already mentioned above. The essential oils of lemon, orange, and cinnamon influence the intestinal microbiota in the same way the essential compounds of coriander inhibit the activity of gastrointestinal pathogenic bacteria. The essential compound of rosemary oil has an anti-Warburg effect on gastric carcinoma, dose, and time. And a thymol compound-dependent cytotoxic effect on cancer cell lines PC-3, DU145, MDA-MB-231, and KLN205.

표 3은 위에서 이미 언급한 에센셜 오일의 다양한 오믹스 메커니즘을 강조합니다. 레몬, 오렌지, 계피의 에센셜 오일은 고수풀의 에센셜 화합물이 위장병 유발 세균의 활동을 억제하는 것과 같은 방식으로 장내 미생물에 영향을 미칩니다. 로즈메리 오일의 에센셜 화합물은 위암에 대한 항워버그 효과를 가지며, 용량과 시간에 따라 효과가 달라집니다. 그리고 티몰 화합물은 암세포주 PC-3, DU145, MDA-MB-231, KLN205에 세포 독성 효과가 있습니다.

Table 3.

Essential oils and omics mechanism.

Essential oilHealth actionOmics of the study epigenetics, microbiota, metabolomics, genomicsOmics mechanism of actionType of studyStudy nameRelevant resultsReference

1	Lemon	Intestinal limonin boosts immunity by optimizing macronutrient metabolism.	Microbiota	16S rRNA gene analysis revealed that intestinal microbial diversity (α-diversity and b-diversity) was significantly modified by limonin intervention	Gut microbiota in mice	The gastrointestinal fate of limonin and its effect on the intestinal microbiota in mice	Microbial species richness increased significantly with limonin treatment (limonene being its main active compound), while low diversity was observed in obese mice fed a high-fat, high-sugar diet.	(25)
2	Orange	Orange essential oil, limonene, linalool and citral decrease short-chain fatty acids in intestines.	Microbiota	The effect of essential oil, linalool and citral on immune organ index, IgM and IL-2 was not significant (p > 0.05). A significant increase (p < 0.05) of H + K + -ATPase activity, IgA, IgG, and IL-2 in the limonene group.	Gut microbiota in mice	Effects of orange essential oil on intestinal microbiota in mice	Orange essential oil could affect the intestinal microbiota of mice and improve the relative abundance of Lactobacillus. This indicated a more obvious effect of limonene in addition to linallool and citral, on intestinal bacteria, as well as changes in blood immune index and short-chain fatty acids in mice.	(27)
3	Rosemary	Rosmarinic acid inhibits Warburg effect in gastric cancer cells.	Genomics	Protein expression‎ was determined by Western blot assay. Mouse xenograft models were established using MKN45 cells to eval‎uate the anti-Warburg effect on gastric carcinoma in vivo.	Gastric cancer cells	Anti-Warburg effect of rosmarinic acid through miR-155 in gastric cancer cells	Rosmarinic acid suppressed glucose uptake and lactate production and inhibited the expression‎ of the transcription factor hypoxia-inducible factor-1α. Inflammation promoted the Warburg effect in cancer cells. As expected, it inhibited pro-inflammatory cytokines and inflammation-related microRNAs.	(28)
4	Rosemary	Rosemary extracts degrade androgen receptors and reduce prostate cancer cell viability.	Microbiota	Standardized rosemary extract decreases the expression‎ of the androgen receptor which appears to be regulated by the expression‎ of CHOP/GADD153	Two human prostate cancer cell lines, 22Rv1 and LNCaP.	Rosemary (Rosmarinus officinalis) extract modulates CHOP/GADD153 to promote androgen receptor degradation and decrease xenograft tumor growth	Significant modulation of endoplasmic reticulum stress proteins was observed in cancer cells. This biphasic response suggests that standardized rosemary extract may preferentially target cancer cells rather than “normal” cells. This study indicated that the main active compound was carnosic acid.	(29)
5	Thyme	Thyme essential oil demonstrates antiproliferative and cytotoxic activity against leukemia, breast and lung cancer cell lines.	Genomics	Detection of cytotoxic concentration range and detection of antiproliferative activity of essential oil	Malignant cell lines (MOLT-4, MCF-7 and H460).	Detection of cytotoxicity of Thymus vulgaris L. essential oil in brine shrimp nauplii and cancer cell lines	Thymol (main active compound) induced a dose-dependent inhibition of cell proliferation in all tumor cell lines. Dose-dependent toxicity confirmed that the brine shrimp lethality test is a suitable method for preliminary toxicity testing of Thymus vulgaris L. essential oil on tumor cell lines.	(30)
6	Thyme	Thymol oil induces apoptosis in cancer cells in a time and dose dependent manner.	Genomics	Determine the antiproliferative activity and apoptotic effect of thymol in prostate cancer (PC-3, DU145), breast cancer (MDA-MB-231) and lung cancer (KLN205) cell lines.	Prostate cancer, breast cancer and lung cancer cell lines	Rosemary (Rosmarinus officinalis) extract modulates CHOP/GADD153 to promote androgen receptor degradation and decrease xenograft tumor growth	The study showed the dose-and time-dependent cytotoxic effect of thymol on cancer cell lines PC-3, DU145, MDA-MB-231 and KLN205. Thymol significantly induced apoptosis in all groups in a dose-dependent manner.	(26)
7	Cinnamon	Cinnamon essential oil has antimicrobial and anti-inflammatory effects that protect against inflammatory bowel disease.	Microbiota	The antibacterial activity of cinnamon is attributed to the destruction of the bacterial membrane, modification of the lipid profile and inhibition of cell division. Suppressed the expression‎ of TLR4, myeloid differentiation factor 88 and nuclear factor kinase.	Mouse model	Effect of cinnamon essential oil on gut microbiota in the mouse model of dextran sodium sulfate-induced colitis	Treatment with CEO (main compound Cinnamaldehyde (68.95%)) improved symptoms of DSS-induced colitis in mice, as demonstrated by a decrease in body weight loss and disease activity index scores.	(31)
8	Thyme	Thymol protects colon epithelial cells from oxidative DNA damage, while geraniol protects against DNA methylation damage.	Genomics	The genoprotective effects of essential oil compounds against oxidative and methylating damage were eval‎uated by the comet assay in HT-29 colorectal adenocarcinoma cells. Most of these were cytotoxic to HT-29 cells at 250 ppm or higher after 24 h of exposure.	The comet assay in HT-29 colorectal adenocarcinoma cells.	Effects of Essential Oil Compounds Against Oxidative and Methylated DNA Damage in Human Colon Cancer Cells	Thymol was the most protective compound against oxidative DNA damage and geraniol also protected cells against DNA methylation damage. Other notable compounds were nerolidol, geraniol, methyl isoeugenol, eugenol, linalool and a commercial mixture (Agolin).	(32)
9	Rosemary	Rosmarinus officinalis compounds like rosmarinic acid, carnosic acid and carnosol give it unique therapeutic effects and increase metabolite production.	Metabolomics	The identification and regulation of the biosynthetic pathways and genes will enable the large-scale production of these compounds	Metabolomics and transcriptomics data of R. officinalis were retrieved from the Microbial and Eukaryotic Systems Resource Database.	System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites	The core genes include copalyl diphosphate synthase (CDS), phenylalanine ammonia lyase (PAL), cineol synthase (CIN), rosmarinic acid synthase (RAS), tyrosine aminotransferase (TAT), cinnamate 4-hydroxylase (C4H) and MYB58 are responsible for the biosynthesis of important secondary metabolites.	(33)

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5. Cinnamon

5.1. Health benefits

In a study by Mohammed et al. (19), six groups of male rats were treated orally for 4 weeks. The control and STZ-treated groups were compared with groups that received low or high doses of cinnamon oil emulsion (COE; 200 or 400 mg/kg Bw) and groups of STZ-treated rats that received COE in low or high doses. The results showed a significant decrease in fasting blood glucose levels, plasma C-peptide, serum triglycerides, total cholesterol, and blood urea nitrogen, with a substantial increase in high-density lipoproteins after 35 days. Glucose tolerance improved, and an increase in pancreatic islet β cells was observed. Both doses improved glucose, insulin, SOD, GSH, amylase, lipid profile, and hepatic MDA levels. Positive histological changes were also observed in the liver and pancreas. The current results revealed that cinnamon oil emulsion (COE) produced more than 1% of total volatile compounds, and GC/MS identified 16 compounds. More than 86% of these volatiles originated from 5 volatiles and included cinnamaldehyde (88.7%), 1,8 cineole (2.02%), acetic acid, 1,7,7trimethylbicyclo [2.2.1] heptyl ester (1.79%), α-Pinene (1.45%), and α-Terpineol (0.92%). This research aimed to examine the potential of essential oils as antitumor agents. In vitro models, including phosphomolybdenum, DPPH, and H2O2 methods, were used to achieve this. This study used BHT (butylhydroxytoluene) and ascorbic acid (vitamin C) as positive controls for comparison (20).

5.2. Food benefits

This review focuses on analyzing the effects of cinnamon oil when used as an additive in poultry feed, addressing its influence on various aspects such as bird performance, carcass characteristics, meat quality, its impact on cholesterol reduction, its antioxidant activity, its effects on immunity and considerations related to microbiology. The key results of this research indicate that including cinnamon essential oil extracts as additives in poultry feed carries notable benefits in terms of improved performance, reduced blood cholesterol levels, and increased activity: antioxidant, immunity booster, and favorable microbiological considerations. Cinnamon could represent a viable alternative to antibiotics, providing excellent safety in aspects related to animal health, the environment, and the economy in the poultry industry. Furthermore, it was observed that the main component of cinnamon oil is Cinnamaldehyde, which approximately represents Cinnamaldehyde (88.2%), eugenol (1.0%), and benzyl alcohol (8.0%) in its composition (34).

5.3. Actions in health

One of the potential health benefits is protection against colitis, which is an anti-inflammatory bowel disease. This is related to the mechanism of action, antimicrobial activity, and anti-inflammatory effect of cinnamon due to the alteration in the bacterial membrane, modifications in the lipid profile, and the inhibition of cell division, as shown in a mouse model study. Cinnamomum osmophloem reduced the expression‎ of Toll-like receptor 4, myeloid adapter protein 88, and nuclear kinase in mice with colitis that had received endotoxin, suggesting an anti-inflammatory effect. In addition, it was identified that the predominant active component in cinnamon essential oil is cinnamaldehyde, which constitutes approximately 68.95% of its composition (31).

5. 계피

5.1. 건강상의 이점

모하메드(Mohammed) 외의 연구(19)에 따르면, 6개 그룹의 수컷 쥐에게 4주 동안 경구 투여를 실시했습니다. 대조군과 STZ 투여군을 저용량 또는 고용량 계피 오일 에멀젼(COE; 체중 1kg당 200mg 또는 400mg) 투여군과 비교했고, STZ 투여 쥐의 COE 투여군을 저용량 또는 고용량 투여군과 비교했습니다. 그 결과, 35일 후 공복 혈당 수치, 혈장 C-펩타이드, 혈청 중성지방, 총 콜레스테롤, 혈액 요소 질소가 현저하게 감소하고 고밀도지단백질이 현저하게 증가했습니다. 포도당 내성이 개선되었고, 췌장 섬 β세포의 증가가 관찰되었습니다. 두 용량 모두 포도당, 인슐린, SOD, GSH, 아밀라제, 지질 프로필, 간 MDA 수치를 개선했습니다. 간과 췌장에서도 긍정적인 조직학적 변화가 관찰되었습니다. 현재 결과에서는 계피 오일 에멀젼(COE)이 총 휘발성 화합물의 1% 이상을 생성하고, GC/MS가 16가지 화합물을 확인한 것으로 나타났습니다. 이 휘발성 성분 중 86% 이상이 5가지 휘발성 성분에서 유래되었으며, 그 성분에는 신남알데히드(88.7%), 1,8-시네올(2.02%), 아세트산, 1,7,7-트리메틸바이사이클로[2.2.1]헵틸에스터(1.79%), α-피넨(1.45%), α-테르피네올(0.92%)이 포함되어 있습니다. 이 연구는 항암제로서의 에센셜 오일의 잠재력을 조사하는 것을 목표로 했습니다. 이를 위해 포스포몰리브덴, DPPH, H2O2 방법을 포함한 체외 모델을 사용했습니다. 이 연구에서는 비교를 위해 BHT(부틸하이드록시톨루엔)와 아스코르브산(비타민 C)을 양성 대조군으로 사용했습니다(20).

5.2. 식품의 이점

이 리뷰는 가금류 사료에 첨가제로 사용될 때 계피 오일이 가지는 효과를 분석하는 데 초점을 맞추고 있으며, 가금류의 성능, 도체 특성, 육질, 콜레스테롤 감소에 미치는 영향, 항산화 활성, 면역에 미치는 영향, 미생물학 관련 고려 사항 등 다양한 측면에 미치는 영향을 다룹니다. 이 연구의 주요 결과는 계란 생산에 계피 에센셜 오일 추출물을 첨가제로 사용하면 생산성 향상, 혈중 콜레스테롤 수치 감소, 항산화, 면역력 강화, 미생물학적 고려 사항 개선 등 상당한 이점이 있다는 것을 보여줍니다. 계피는 항생제에 대한 효과적인 대안이 될 수 있으며, 가금류 산업의 동물 건강, 환경, 경제와 관련된 측면에서 탁월한 안전성을 제공합니다. 또한, 계피 오일의 주성분은 계피알데하이드(Cinnamaldehyde)로, 계피알데하이드(88.2%), 유제놀(1.0%), 벤질알코올(8.0%)로 구성되어 있는 것으로 관찰되었습니다(34).

5.3. 건강에 미치는 영향

건강에 도움이 될 수 있는 잠재적인 이점 중 하나는 항염증성 장 질환인 대장염을 예방하는 것입니다. 이것은 마우스 모델 연구에서 나타난 바와 같이, 박테리아 막의 변화, 지질 프로파일의 변형, 세포 분열의 억제로 인해 계피의 작용 메커니즘, 항균 작용, 항염증 효과와 관련이 있습니다. 시나몬 오스모플로에름은 내독소 투여 후 대장염이 발생한 생쥐의 Toll-like receptor 4, myeloid adapter protein 88, nuclear kinase의 발현을 감소시켜 항염증 효과를 시사합니다. 또한, 시나몬 에센셜 오일의 주성분은 약 68.95%를 차지하는 시나멜알데하이드(cinnamaldehyde)인 것으로 밝혀졌습니다(31).

6. Cilantro

6.1. Health benefits

In a study conducted by Helal et al. (21) on the motility of third-instar larvae (L3) belonging to the Trichostrongylidaeindicate that these essential oils and their components, such as linalool (68.03%), α-tinene (9.71%), γ-terpinene (10.48%) and camphor (11.76%), could effectively combat infections caused by helminths.

Moreover, studies have shown that essential oils, including cilantro oil, can effectively hinder the growth of clinical strains of dermatophytes belonging to two primary genera, Microsporum and Trichophyton, for up to 21 days. It is worth noting that accurately identifying the specific individual is crucial (22).

6.2. Food benefits

The antioxidant, antimicrobial, and anti-biofilm properties of the essential oil obtained from cilantro (Coriandrum sativum L.) are the most studied concerning its possible application in food. The main volatile components identified in coriander essential oil in an in vitro study were mainly β-linalool, which accounted for 66.07% of the total content, showing a high antioxidant activity with an inhibition percentage of 51.05% in eliminating radicals. Its antibacterial activity presented the most effective against B. subtilis, followed by S. maltophilia and Penicillium expansum (9). An in vivo study assessed the effectiveness of adding 1% coriander oil to tilapia feed to enhance health and immunity against bacterial infections. The key compounds in this oil were linalool and geranyl acetate (10).

7. Cumin

7.1. Health benefits

Recent studies have shown that cumin oil can potentially suppress neutrophil activation, which could be beneficial in treating inflammatory diseases that involve high levels of neutrophil activity. This includes respiratory burst and degranulation induced by formylpeptide receptor agonists fMLF/CB and MMK1 in human neutrophils. These effects’ mean inhibitory concentration (IC50) ranges from 3.8 to 17.2 μg/mL. Additionally, it has been noted that cumin oil contains a high percentage of cumin aldehyde, which makes up 49.9% of its composition (11). Morovati et al. (12) found that cumin essential oil significantly reduced diastolic blood pressure in patients with metabolic syndrome after 8 weeks of treatment.

8. Parsley

8.1. Health benefits

Parsley essential oil exhibits an antioxidant profile due to its higher percentage of DPPH radical inhibition and FRAP value; however, it also showed pro-oxidative behavior according to the TBARS test. On the other hand, parsley essential oil demonstrates more significant bacterial activity after lavender. The main components were myristicin (36.15%), apiole (20.97%), α-pinene (15.47%), and β-pinene (10.43%). The presence of alkyltetramethoxybenzene, limonene and elemicin (6.45, 4.74 and 2.74%, respectively) was also relevant (13).

9. Lemon

9.1. Health benefits

It has been shown that the essential oil obtained by hydrodistillation of discarded lemon leaves contains enough chemicals can inhibit the growth of harmful microorganisms such as C. albicans, L. monocytogenes, and S. aureus. The oil’s most abundant components are limonene (with a concentration of 260.7 mg/mL), followed by geranial (102.6 mg/mL) and neral (88.3 mg/mL). The study also found that a concentration of 25 μM of the oil led to a significant reduction in cell viability, with a 33% decrease in HeLa cells and a 27% decrease in A375 cells. This was also accompanied by notable changes in cellular morphology (14).

The main components of lemon essential oil responsible for its antibacterial and antioxidant properties are terpenoids, with d-limonene being the most abundant. D-limonene exhibits the highest antioxidant capacity and effectively removes DPPH radicals (16).

9.2. Food benefits

In the food industry, lemon essential oil’s antioxidant and antimicrobial properties can be used as a preservative. According to Ben Hsouna et al. (15), the application of this oil at concentrations of 0.06 and 0.312 mg/g presents promising potential for the prevention of contamination and the development of pathogenic bacteria, especially L. monocytogenes, which opens new perspectives in this field.

9.3. Action in health

The effect of limonin on the intestinal microbiota has been investigated in mice, and a significant increase in the diversity of the microbiota present in the colon of mice fed limonin has been observed. Thus, it was highlighted that the composition of the intestinal microbiota community was different compared to the control group. A prediction was made that limonin would positively affect the regulation of amino acid metabolism, lipids, and immune system function. It is highlighted that it can significantly suppress diseases related to the immune system and markers of infectious diseases based on its influence on the intestine (25).

10. Orange10.1. Health benefits

The Citrus sinensis peel oil has potential antibacterial, antifungal, and antiparasitic effects, according to a study conducted by Anwar et al. (18). The analysis showed that the maximum inhibition zone diameter was 14 mm against E. coli, and the minimum was 10 mm against S. agalactiae. Moreover, the oil was 60% effective in inhibiting leishmaniasis at a 50 μg/mL concentration after 48 h of incubation. The oil’s antimicrobial properties were also demonstrated, suggesting its possible use as a natural food preservative or an effective treatment against various pathogenic organisms. The oil contains β-pinene (0.55%), limonene (96–98%), α-pinene (0.29%), myrcene (1.3–1.45%), and octanol (0.37–0.53%).

10.2. Food benefits

A study assessed commercial orange essential oils’ chemical compositions, antioxidant, and anti-pathogenic properties [Citrus sinensis (L.) Osbeck]. The study used cold pressing (EOP) and cold pressing followed by steam distillation (EOPD). The analysis revealed that both essential oils contained a high percentage of monoterpene hydrocarbons, mainly limonene (89.8 to 90.4%) and myrcene (3.1 to 3.2%). Although both essential oils had similar reducing capacities, EOP showed a more remarkable ability to eliminate free radicals. Regarding anti-pathogenic properties, both essential oils inhibited the biomass and cellular viability of Staphylococcus aureus and Pseudomonas aeruginosa in their biofilms. Additionally, both methods effectively reduced the production of elastase, pyocyanin, and quorum-sensing autoinducers, particularly in Gram-negative bacteria. These findings indicate that EOP and EOPD demonstrate significant antioxidant and anti-pathogenic properties (17).

10.3. Action in health

A recent study examined the effects of administering orange essential oil, as well as limonene, linalool, and citral, directly into the stomachs of mice. The researchers were interested in understanding how these substances might affect the mice’s intestinal microbiota and biochemical parameters. The study found that all four substances could influence the mice’s intestinal microbiota composition, with the relative proportion of Lactobacillus increasing in response to treatment. However, the mice that received limonene had a notably different bacterial composition in their cecum and colon than the other groups. These findings suggest that limonene may have a more pronounced effect on intestinal bacteria, which can lead to significant changes in blood immunological markers and short-chain fatty acid levels in mice (27).

11. Thyme

11.1. Health benefits

Thyme essential oil (TEO) has health benefits due to its bioactive compounds. TEO nanoemulsion improves its biological activity and antioxidant properties and protects against oxidative damage and genotoxicity caused by TiO2-NP. TEO contains 17 bioactive compounds, with thymol and carvacrol being the main components (24).

11.2. Action in health

Thymol has been found to impact prostate cancer (PC-3), breast cancer (MDA-MB-231), and lung cancer cells. The antiproliferative activity of cancer cells varies depending on the dose and time of exposure. Thymol also significantly induces apoptosis in all groups, with the intensity of the response varying depending on the dose administered (26).

Likewise, the antiproliferative activity has been eval‎uated (in vitro) using three human tumor cell lines: MCF-7 (breast adenocarcinoma), H460 (lung carcinoma), and MOLT-4 (acute lymphoblastic leukemia) using the MTT assay, demonstrating a dose-dependent inhibition of cell proliferation in all tumor cell lines eval‎uated, and differential sensitivity between them. The main components of the essential oil included thymol (36.7%), p-cymene (30.0%), γ-terpinene (9.0%), and carvacrol (3.6%) (30).

Understanding oils’ effects on our health is crucial, particularly in preventing cellular toxicity and genotoxicity. A recent study found that essential oil compounds can offer protective benefits against oxidative and methylating damage, as seen through comet assays on colorectal adenocarcinoma HT-29 cells. While most of these compounds were found to be cytotoxic to HT-29 cells, they only reached cytotoxic levels at doses equal to or greater than 250 ppm after exposure for 24 h. The study identified thymol as the most effective component in protecting DNA against oxidative damage, while geraniol also showed promise in protecting against DNA methylation damage. This research highlights the potential of essential oil compounds, especially thymol, in protecting the colonic epithelium against oxidative DNA damage and geraniol against DNA methylation damage (32).

12. Rosemary

12.1. Health benefits

A study by Christopoulou et al. (23) assessed the chemical composition, genotoxicity, and antimicrobial, antiviral, and antioxidant properties of certain substances. The results showed that the essential oil, at concentrations of up to 5%, and the extract, ranging from 25 to 90%, did not exhibit any genotoxic effects. The essential oil and the extract also demonstrated antiviral, antifungal, and antioxidant properties. Specifically, the extract exhibited notable antibacterial properties, while the essential oil was primarily effective against S. aureus. The essential oil mainly comprised monoterpenes, constituting 95.57%, whereas sesquiterpenes only represented 4.24%.

12.2. Action in health

A study on rosmarinic acid (RA) and the anti-Warburg effect in gastric carcinoma suggested that rosemary oil may reduce glucose uptake and lactate production in cancer cells. It was found that RA inhibits the expression‎ of hypoxia-inducible factor 1α, which is involved in the glycolytic pathway. In cancer cells, inflammation promotes the Warburg effect. However, RA reduces the production of pro-inflammatory cytokines and inflammation-related microRNAs, which suggests that RA could suppress the Warburg effect through an inflammatory pathway related to interleukin (IL)-6 and the transcription factor STAT3 (28).

Using essential oil to improve the functioning of the endoplasmic reticulum can be a helpful health action in reducing the viability of prostate cancer cells and promoting the degradation of androgen receptors. A study eval‎uated the effects of rosemary extract, standardized in carnosic acid, on two types of human prostate cancer cells, 22Rv1 and LNCaP, and prostate epithelial cells collected from two patients undergoing radical prostatectomy. The study found that cancer cells significantly altered endoplasmic reticulum stress proteins, while normal prostate epithelial cells did not suffer endoplasmic reticulum stress. This two-stage response suggests that standardized rosemary extract might be a preferable treatment option for cancer cells rather than normal cells.

On the other hand, some results suggest that the identified core genes, such as copalyl diphosphate synthase, phenylalanine ammonia-lyase, cineole synthase, rosmarinic acid synthase, tyrosine aminotransferase, cinnamate 4-hydroxylase, and MYB58, could play a crucial role in the metabolism of Rosmarinus officinalis. This genetic analysis provides valuable information for genetic and metabolic engineering research to improve the biosynthesis of secondary metabolites in Rosmarinus officinalis (33).

13. Discussion

This review emphasizes the significant advancements of essential oils in various fields, such as medicine and the food industry, achieved through in vitro, in vivo, and cell line studies. These breakthroughs provide valuable insights that support the continuation of research in these areas. For instance, the study eval‎uates the impact of rosemary essential oil extract on reducing prostate cancer cells (28). Moreover, the effects of cinnamon oil as a food additive for poultry are examined. The study explores its influences on avian performance, meat quality, cholesterol reduction, antioxidant activity, and immunity (35).

Insufficient literature exists on essential oils concerning innovative omics action mechanisms, more relevant applications in food to ensure quality, and their benefits and impact on health. Therefore, it is recommended that future research should conduct studies using cell lines (in vivo). This approach promotes more natural additives, thereby reducing the intake of processed additives that may adversely affect human health in the long run. These limitations are significant as they restrict understanding of essential oils’ potential benefits to improve the quality of life and address specific health-related needs in the food industry.

The potential health benefits of rosemary oil have been extensively studied and documented. Compounds included in rosemary oil, notably oleic and linoleic acid, are known to benefit cardiovascular health (36). Evidence is limited for the effects of rosemary oil on glucose. Further research, with more rigorous experimental designs and in larger populations, is required to reach more definitive conclusions about these properties (35, 37–39). One of the main factors contributing to rosemary oil’s potential usage in pharmacological and biotechnological applications is its biological qualities (8, 40). For example, natural antioxidants such as rosemary extracts have shown high thermal resistance and superior antioxidant activity compared to synthetic antioxidants (41). This makes them beneficial for preserving the quality of oils, particularly in high-heat cooking processes like frying (42, 43). Beyond acting as food preservatives, rosemary oil components may have direct health advantages. The oil’s anti-inflammatory effects may also promote brain health by preventing neuronal cell damage (44–46). Ongoing research reveals rosemary oil’s multipronged therapeutic potential—from enhancing heart health to shielding brain function to inhibiting tumor growth (47–49).

Several studies have been conducted that provide convincing evidence of the usefulness of cinnamon essential oil, but most are in vitro or animal research. More large-scale, high-quality human clinical trials are still needed to confirm‎ the effects, establish the optimal dose, assess long-term safety, etc. Mechanistic studies have elucidated certain pathways, such as modulation of glucose transporters, antioxidant enzymes and inflammatory markers that underlie the observed effects. However, further work is needed to elucidate the full pharmacokinetic and pharmacodynamic profile, especially of key actives such as cinnamaldehyde (50–53).

Most research has focused specifically on cinnamon essential oil or cinnamaldehyde-standardized bark extracts. Comparisons of potency, bioavailability, and synergies between components require further research. Despite promising gastrointestinal effects, more extensive studies through rigorous randomized controlled trials on outcomes such as ulcerative colitis, irritable bowel syndrome, and dysbiosis are needed before clinical use can be recommended (54, 55). Safety, drug interactions, and contraindications have yet to be fully established.

Some studies demonstrate the efficacy of coriander oil against organisms such as fungi and helminths and agree that linalool e is the chemically essential compound (56). However, other chemotypes and oil compositions should also be examined, e.g., those richer in decanal, borneol, or geranyl acetate for which these comparative studies would demonstrate bioactivity that may reveal differential effects (57, 58). In addition, more detailed analyses, especially in food matrices exploring lipid peroxidation, protein oxidation, effects on shelf life, etc., would provide additional information along with limited safety studies (59–61). Toxicity, pharmacokinetic, and residue level studies in food-producing animals could allow the administration of higher standardized doses to enhance health effects (62–65). Finally, few in vivo studies explore the bioavailability, metabolism, and excretion of key actives such as linalool and the impact of long-term repeated dosing. Such pharmacokinetic data can help correlate in vitro and clinical results (64, 66).

Finally, lemon essential oil, the main component, D-limonene, boosts antimicrobial and antioxidant effects (15, 67, 68). However, further studies are needed on possible synergies with other components such as geranial (69, 70). In addition, rigorous human clinical trials are required to validate efficacy as a natural food preservative and to determine optimal dosage (71–73). The effect on the gut microbiome is promising, but it is not yet clear what specific changes in bacterial composition drive the observed health outcomes (68, 74).

13. 토론

이 리뷰는 체외, 생체, 세포주 연구를 통해 의학 및 식품 산업 등 다양한 분야에서 에센셜 오일이 이룬 상당한 발전을 강조합니다. 이러한 돌파구는 이러한 분야에서 연구를 계속할 수 있도록 지원하는 귀중한 통찰력을 제공합니다. 예를 들어, 이 연구는 로즈마리 에센셜 오일 추출물이 전립선암 세포를 줄이는 데 미치는 영향을 평가합니다(28).

또한, 가금류용 식품 첨가물로서의 계피 오일의 효과를 조사합니다. 이 연구는 조류의 성능, 육질, 콜레스테롤 감소, 항산화 작용, 면역력에 미치는 영향을 조사합니다(35).

혁신적인 옴니믹스 작용 메커니즘, 품질 보장을 위한 식품에서의 더 관련성 있는 응용, 그리고 건강에 미치는 이점과 영향에 관한 에센셜 오일에 관한 문헌은 충분하지 않습니다. 따라서 향후 연구에서는 세포주(생체 내)를 이용한 연구를 수행하는 것이 좋습니다. 이러한 접근 방식은 더 자연적인 첨가제를 촉진하여 장기적으로 인간의 건강에 악영향을 미칠 수 있는 가공 첨가제의 섭취를 줄입니다. 이러한 제한은 에센셜 오일이 삶의 질을 향상시키고 식품 산업에서 특정 건강 관련 요구를 해결하는 데 있어 잠재적인 이점을 이해하는 데 제한을 두기 때문에 중요합니다.

로즈마리 오일의 잠재적인 건강상의 이점은 광범위하게 연구되고 문서화되었습니다. 로즈마리 오일에 포함된 화합물, 특히 올레산과 리놀레산은 심혈관 건강에 이로운 것으로 알려져 있습니다(36). 포도당에 대한 로즈마리 오일의 효과에 대한 증거는 제한적입니다. 이러한 특성에 대한 보다 확실한 결론을 얻기 위해서는 더 엄격한 실험 설계와 더 많은 인구를 대상으로 한 추가 연구가 필요합니다(35, 37-39). 로즈마리 오일이 약리학 및 생명공학 분야에서 사용될 수 있는 잠재력의 주요 요인 중 하나는 생물학적 특성입니다(8, 40). 예를 들어, 로즈마리 추출물과 같은 천연 항산화제는 합성 항산화제에 비해 높은 열 저항성과 우수한 항산화 활성을 나타냅니다(41). 따라서 특히 튀김과 같은 고열 조리 과정에서 오일의 품질을 보존하는 데 유용합니다(42, 43). 로즈마리 오일의 성분은 식품 보존제 역할을 할 뿐 아니라 건강에 직접적인 이점을 제공할 수 있습니다. 오일의 항염증 효과는 또한 신경 세포 손상을 방지하여 뇌 건강을 증진할 수 있습니다(44-46). 지속적인 연구에 따르면 로즈마리 오일은 심장 건강 증진, 뇌 기능 보호, 종양 성장 억제 등 다양한 치료 효과를 나타낼 수 있는 것으로 밝혀졌습니다(47-49).

계피 에센셜 오일의 유용성에 대한 설득력 있는 증거를 제공하는 여러 연구가 수행되었지만, 대부분 체외 또는 동물 연구에 불과합니다. 효과를 확인하고, 최적의 용량을 설정하고, 장기적인 안전성을 평가하기 위해서는 더 많은 대규모의 고품질 인체 임상 시험이 여전히 필요합니다. 기계적 연구에 의해 관찰된 효과의 기초가 되는 포도당 수송체, 항산화 효소, 염증 표지자의 조절과 같은 특정 경로가 밝혀졌습니다. 그러나, 특히 계피 알데하이드(50-53)와 같은 주요 활성 물질의 전체 약동학 및 약력학적 프로파일을 밝히기 위해서는 더 많은 연구가 필요합니다.

대부분의 연구는 계피 에센셜 오일 또는 계피 알데하이드 표준화 껍질 추출물에 초점을 맞추고 있습니다. 성분 간의 효능, 생체 이용률, 시너지 효과 비교를 위해서는 추가 연구가 필요합니다. 위장 효과가 유망함에도 불구하고, 임상 사용을 권장하기 전에 궤양성 대장염, 과민성 대장 증후군, 장내 세균 불균형과 같은 결과에 대한 엄격한 무작위 대조 시험을 통한 더 광범위한 연구가 필요합니다(54, 55). 안전성, 약물 상호 작용, 금기 사항은 아직 완전히 확립되지 않았습니다.

일부 연구에서는 고수기름이 곰팡이, 기생충 등의 유기체에 효과가 있다는 것을 입증하고 있으며, 리날로올 e가 화학적으로 필수적인 화합물이라는 데 동의하고 있습니다(56). 그러나 데칸알, 보르네올, 또는 제라니엘 아세테이트가 더 풍부한 오일 조성물 등 다른 화학 유형도 조사해야 합니다. 이러한 비교 연구를 통해 차별화된 효과를 나타낼 수 있는 생체활성을 입증할 수 있을 것입니다(57, 58). 또한, 특히 지질 과산화, 단백질 산화, 유통 기한에 미치는 영향 등을 조사하는 식품 매트릭스에서 더 자세한 분석이 제한적인 안전성 연구와 함께 추가 정보를 제공할 수 있습니다(59-61). 식품 생산 동물에 대한 독성, 약동학, 잔류 수준 연구를 통해 건강 효과를 향상시키기 위해 더 높은 표준 용량을 투여할 수 있습니다(62-65). 마지막으로, 생체 내 연구에서는 리날로올과 같은 주요 활성 성분의 생체 이용률, 대사, 배설과 장기간 반복 투여의 영향을 조사하는 연구는 거의 없습니다. 이러한 약동학 데이터는 시험관 내 결과와 임상 결과를 연관시키는 데 도움이 될 수 있습니다(64, 66).

마지막으로, 레몬 에센셜 오일의 주성분인 D-리모넨은 항균 및 항산화 효과를 강화합니다(15, 67, 68). 그러나 제라니올(69, 70)과 같은 다른 성분과의 시너지 효과에 대한 추가 연구가 필요합니다. 또한, 천연 식품 방부제로서의 효능을 검증하고 최적의 용량을 결정하기 위해서는 엄격한 인체 임상 시험이 필요합니다(71-73). 장내 미생물 군집에 미치는 영향은 유망하지만, 관찰된 건강 결과를 이끌어내는 세균 구성의 구체적인 변화가 무엇인지 아직 명확하지 않습니다(68, 74).

14. Conclusion

Essential oils extracted from plants have been valued for their medicinal properties for centuries. Modern scientific research is now unraveling the composition and bioactivity of these complex natural extracts, providing insights into how essential oils might be used to help promote health and well-being. Based on the evidence summarized in the provided documents, several key conclusions regarding cinnamon, cilantro, cumin, parsley, lemon, orange, thyme, and rosemary essential oils can be drawn.

Firstly, these essential oils demonstrate varying degrees of antioxidant, antimicrobial, anti-inflammatory, or other beneficial biological effects. These effects are mediated by bioactive phytochemicals in the oils, such as cinnamaldehyde, linalool, limonene, thymol, and carnosic acid. The impacts of oils depend not just on their chemical composition but also on factors like genetics and growth conditions of the source plants. Standardization and quality control are thus important when studying and applying essential oil preparations. Secondly, many oils show promise as natural food preservatives—for example, inhibiting foodborne pathogens like Escherichia coli and Staphylococcus aureus. If efficacious and safe, plant-derived antimicrobials could replace synthetic additives. Areas needing more research include determining effects on food quality and nutritional content during storage.

Some of the main health benefits supported by current evidence are cinnamon oil improving glucose control and blood lipid levels; cilantro oil having parasite-killing properties; cumin oil reducing blood pressure; thyme oil protecting against oxidative cell damage and DNA mutations; and rosemary and thyme oils inhibiting cancer cell proliferation and viability. Further mechanisms of action are being elucidated—for instance, rosemary compounds may suppress altered metabolic pathways in tumor cells. More clinical trials are warranted to verify therapeutic efficacy and safety. An emerging area of research is how essential oil components like limonene and thymol influence intestinal microbiota populations.

So, essential oils are promising candidates for health promotion and disease treatment. However, converting traditional claims into evidence-based applications requires meticulous methodology. Key priorities in the future are clinical eval‎uations demonstrating efficacy, standardization, and quality control of oil preparations, untangling oils’ mechanisms of action, and further analyzing effects on human microbiota. With rigorous science illuminating their real therapeutic potential, essential oils could reveal themselves to be far more than just pleasant natural scents.

14. 결론
식물에서 추출한 에센셜 오일은 수세기 동안 약효 성분으로 높이 평가되어 왔습니다. 현대 과학 연구는 이러한 복합적인 천연 추출물의 구성과 생체 활성을 밝혀내고 있으며, 에센셜 오일이 건강과 웰빙 증진에 어떻게 도움이 되는지에 대한 통찰력을 제공하고 있습니다. 제공된 문서에 요약된 증거를 바탕으로 계피, 고수, 커민, 파슬리, 레몬, 오렌지, 타임, 로즈메리 에센셜 오일에 관한 몇 가지 주요 결론을 도출할 수 있습니다.

첫째, 이 에센셜 오일은 항산화, 항균, 항염증 또는 기타 유익한 생물학적 효과를 다양한 수준으로 나타냅니다. 이러한 효과는 오일에 함유된 생체 활성 식물성 화학 물질(예: 신남알데히드, 리날로올, 리모넨, 티몰, 카르노산)에 의해 매개됩니다. 오일의 영향은 화학 성분뿐만 아니라 원료 식물의 유전적 특성 및 성장 조건과 같은 요인에 의해 좌우됩니다. 따라서 에센셜 오일 제제를 연구하고 적용할 때는 표준화와 품질 관리가 중요합니다. 둘째, 많은 오일이 대장균과 황색포도상구균과 같은 식중독 유발 병원균을 억제하는 등 천연 식품 방부제로서의 가능성을 보여줍니다. 효능과 안전성이 입증된다면, 식물 유래 항균제는 합성 첨가제를 대체할 수 있을 것입니다. 더 많은 연구가 필요한 분야로는 식품 저장 중 식품의 품질과 영양 성분에 미치는 영향을 확인하는 것이 있습니다.
현재의 증거에 의해 뒷받침되는 주요 건강상의 이점 중 일부는 다음과 같습니다. 계피 오일은 포도당 조절과 혈중 지질 수치를 개선합니다. 고수 오일은 기생충을 죽이는 성질을 가지고 있습니다. 커민 오일은 혈압을 낮춥니다. 타임 오일은 산화적 세포 손상 및 DNA 돌연변이를 방지합니다. 로즈마리와 타임 오일은 암세포의 증식과 생존을 억제합니다. 로즈마리 화합물이 종양 세포의 변화된 대사 경로를 억제할 수 있다는 등, 작용 메커니즘에 대한 추가 연구가 진행되고 있습니다. 치료 효과와 안전성을 검증하기 위해 더 많은 임상 시험이 필요합니다. 최근 연구 분야는 리모넨과 티몰 같은 에센셜 오일 성분이 장내 미생물 군집에 어떤 영향을 미치는지에 대한 것입니다.
따라서, 에센셜 오일은 건강 증진과 질병 치료에 유망한 후보입니다. 그러나 전통적인 주장을 증거 기반 응용으로 전환하려면 세심한 방법론이 필요합니다. 향후 주요 우선 과제는 효능을 입증하는 임상 평가, 오일 제제의 표준화 및 품질 관리, 오일의 작용 메커니즘 파악, 인간 미생물 군집에 대한 영향 분석입니다. 엄격한 과학이 에센셜 오일의 실제 치료 잠재력을 밝혀내면, 에센셜 오일은 단순한 기분 좋은 천연 향기 그 이상의 존재임을 입증할 수 있을 것입니다.

14.1. Limitations

This review is limited by the variability in the chemical composition of essential oils since these can vary according to the species/variety of the plant, growing conditions, time of harvest, extraction method, etc., making standardization and generalization of results difficult. Likewise, there is little information on the absorption, distribution, metabolism, and excretion (pharmacokinetics) of the components of essential oils in humans, and finally, there is not enough scientific evidence on interactions of essential oils with medications, nutrients, and other compounds - nutrient and drug interactions. For data analysis, the use of Cochrane tools to assess the risk of bias in randomized controlled trials, while for observational studies, the quality assessment tool of the US National Institutes of Health was not applied to the results since the included studies are in vitro.

Author contributions

CP-O: Conceptualization, Validation, Data curation, Investigation, Writing – original draft. FG: Conceptualization, Data curation, Investigation, Writing – original draft, Formal analysis, Methodology, Software. CM: Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing. JM: Funding acquisition, Project administration, Supervision, Validation, Writing – review & editing. AO-M: Funding acquisition, Project administration, Supervision, Validation, Writing – review & editing, Conceptualization, Methodology.

Acknowledgments

The authors would like to express their gratitude toward Mary Young and Tamara Packer for their interest in the research and support.

Funding Statement

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by NovaVita S.A. grants for research. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication.

Conflict of interest

CP-O and JLM were employed by company NovaVita.

The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be eval‎uated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

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