Buffer solution 만드는 방법 ( 책보지 않고)

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Buffer solution can be defined as an equilibrium mixture of a conjugate acid-base pair which is capable of resisting substantial; changes in pH upon the addition of small amounts of acidic or basic substances. With some exceptions, either the acid or the base of the conjugate pair is a weak electrolyte.

The Henderson-Hasselbalch equation is a mathematical statement which defines the pH of a solution of a conjugate acid-base pair in terms of the dissociation constant of the weak acid-base pairs and the equilibrium concentrations of the acid and its conjugate base. This equation is extremely useful in calculating the pH of the buffer.

Figure 1: Preparation of a 0.02M Tris buffer at pH 8.0.

Buffer A and B are prepared in the same way except 0.5 mole of NaCl is added to flask B before the volume is brought to 1 L for buffer B.

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