090506 적색과 녹색 LED를 이용한 심비디움 조직배양(09-14)

<?xml:namespace prefix = v ns = "urn:schemas-microsoft-com:vml" /><v:shapetype id=_x0000_t75 stroked="f" filled="f" path="m@4@5l@4@11@9@11@9@5xe" o:preferrelative="t" o:spt="75" coordsize="21600,21600"><v:stroke joinstyle="miter"></v:stroke><v:formulas><v:f eqn="if lineDrawn pixelLineWidth 0"></v:f><v:f eqn="sum @0 1 0"></v:f><v:f eqn="sum 0 0 @1"></v:f><v:f eqn="prod @2 1 2"></v:f><v:f eqn="prod @3 21600 pixelWidth"></v:f><v:f eqn="prod @3 21600 pixelHeight"></v:f><v:f eqn="sum @0 0 1"></v:f><v:f eqn="prod @6 1 2"></v:f><v:f eqn="prod @7 21600 pixelWidth"></v:f><v:f eqn="sum @8 21600 0"></v:f><v:f eqn="prod @7 21600 pixelHeight"></v:f><v:f eqn="sum @10 21600 0"></v:f></v:formulas><v:path o:connecttype="rect" gradientshapeok="t" o:extrusionok="f"></v:path><?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:lock aspectratio="t" v:ext="edit"> <v:shapetype id=_x0000_t75 stroked="f" filled="f" path="m@4@5l@4@11@9@11@9@5xe" o:preferrelative="t" o:spt="75" coordsize="21600,21600"><v:stroke joinstyle="miter"></v:stroke><v:formulas><v:f eqn="if lineDrawn pixelLineWidth 0"></v:f><v:f eqn="sum @0 1 0"></v:f><v:f eqn="sum 0 0 @1"></v:f><v:f eqn="prod @2 1 2"></v:f><v:f eqn="prod @3 21600 pixelWidth"></v:f><v:f eqn="prod @3 21600 pixelHeight"></v:f><v:f eqn="sum @0 0 1"></v:f><v:f eqn="prod @6 1 2"></v:f><v:f eqn="prod @7 21600 pixelWidth"></v:f><v:f eqn="sum @8 21600 0"></v:f><v:f eqn="prod @7 21600 pixelHeight"></v:f><v:f eqn="sum @10 21600 0"></v:f></v:formulas><v:path o:connecttype="rect" gradientshapeok="t" o:extrusionok="f"></v:path><o:lock aspectratio="t" v:ext="edit"></o:lock></v:shapetype> <img src="https://t1.daumcdn.net/cfile/cafe/14173A0E4A01809692" class="tx-daum-image" style="CLEAR: none; FLOAT: none" actualwidth="530" hspace="1" width="530" vspace="1" border="0" /> </o:lock></v:shapetype> <o:p></o:p> Le Van Tuong Huan and Michio Tanaka. 2004. Environ. Contril in Biol. 42(1) 57-64.   Effects of Red and Blue Light-Emitting Diodes on Callus Induction, Callus Proliferation and Protocorm-Like Body Formation from Callus in Cymbidium Orchid   <o:p></o:p> The effects of light generated by red and blue light-emitting diodes (LEDs) on callus induction from protocorm-like body (PLB) segments, callus proliferation and PLB formation from callus in Cymbidium orchid were investigated. The cultures were placed in 'LED PACK 4' incubators under different ratiosof red and blue LED light (100% red LEDs, 75% red LEDs+25% blue LEDs) at 45μmol m-2 s-1 with a 16-h photoperiod or put under plant growth fluorescent (PGF) light with the same light intensity and photoperiod. Among the treatments, 100% red LEDs was the most effective for callus induction from PLB segments. Callus proliferation was best in the 75% red LEDs+25% blue LEDs treatment but was not significantly different from that in PGf light. The highest PLB formation from callus was obtained in 25% red　LEDs. The results suggested that LEDs are the effective light source for callus induction, callus proliferation and PLB formation from callus in Cymbidium orchid.   <o:p></o:p>   심비디움에서 캘러스유도, 캘러스 증식, 원괴체 유사조직 형성에 미치는 적색과 녹색 발광 다이오드의 효과  <o:p></o:p> 심비디움에 있어서 원괴체 유사조직(PLB)에서 캘러스를 유도하고, 캘러스로부터 증식하고 PLB 형성하는데 적색과 청색의 발광 다이오드의 효과를 조사하였다. 16h 광주기, 45μmol m-2 s-1조건에서 적색과 청색의 LED 광(100% 적색, 75% 적색+25% 적색, 50%적색+50%청색, 25%적색+75%청색, 100% 청색)의 비율을 달리하여 ‘LED PACK 4' 챔버에서 배양하고 또한 형광등 하에서도 같은 광주기 조건으로 배양하였다. 처리중에서 100% 적색 LED에서는 PLB로부터 캘러스틀 유도하는데 가장 효과적이었으며. 캘러스 증식은 75%적색+25%청색 LED 조합에서 좋았다. 그러나 형광등 처리와 차이가 유의하지 않았다. 캘러스로부터 가장 많은 PLB 형성은 25%적색+75%청색 LED 조합에서 얻었다. 그 결과 LED는 심비디움에서 캘러스 형성, 캘러스로부터 증식과 PLB 형성에 효과적인 광으로 사료된다.       <a href='javascript:fileFilterViewer("http://cfile258.uf.daum.net/attach/1817320E4A0180269B31C9", "/cfile258/18/17320E4A0180269B31C9", "Effects of red and blue lingt-emitting diodess on callus in.pdf", "grpid%3D19iP6%26fldid%3Dpec%26dataid%3D112%26fileid%3D1%26regdt%3D20090506211654&url=http%3A%2F%2Fcfile258.uf.daum.net%2Fattach%2F1817320E4A0180269B31C9")'><img src="https://t1.daumcdn.net/daumtop_deco/icon/icon.hanmail.net/editor/p_pdf_s.gif" border="0" alt="첨부파일" class="vam"/> Effects of red and blue lingt-emitting diodess on callus in.pdf</a>