090516-Anther culture in connection with induced mutations for rice

[이자현]

Euphytica 120: 401–408, 2001.

Anther culture in connection with induced mutations for rice improvement

Doubled haploids have long been recognized as a valuable tool in plant breeding since it not only offers the quickest
method of advancing heterozygous breeding lines to homozygosity, but also increases the selection efficiency over
conventional procedures due to better discrimination between genotypes within any one generation. Ten cultivars
of japonica rice and nine cultivars of indica rice were eval‎uated for androgenic response. Various doses (10–50 Gy)
of gamma rays were applied to investigate the effect of radiation on callus formation, green plant regeneration and
the frequency of selected doubled haploid mutants. Similarly, the effects of colchicine concentration (10–200 mg/l)
on callus induction, regeneration and fertility of green plants were observed. It was demonstrated that the dose of
20 Gy gamma rays and 30 mg/l concentration of colchicine have significant stimulation effect on regeneration of
green plants from rice anther culture. The high frequency of observed doubled haploidmutants indicates that anther
culture applied in connection with gamma rays is an effective way to improve rice cultivars.

rice 개량을 위해 유도한 돌연변이와 관련된 약배양

Doubled haploid는 homozygosity에 대한 heterozygous 육종라인을 점진하는 가장 빠른 방법을 제공하지 않기 때문에 식물육종에서 유용한 기술로써 오랫동안 인식되어 왔다. 그러나 한 세대 내에서 genotype 사이에 더 나은 구별 때문에 전통적인 과정보다 선택효율이 증가한다.androgenic response을 위해  japonica rice의 10 품종과 indica race의 9품종을 평가하였다. 캘러스 형성, 녹색식물 재생, 선택된 복이배체(변이체)의 빈도에 관한 방사선의 효울을 조사하는데 10-50Gy의 감마선을 사용하였다. 캘러스유도, 녹색식물의 재생과 번식력에 관한 콜히친농도별(10-200mg/l) 효과를 관찰하였다. 20Gy 선량과 콜히친 30gm/l 가 rice의 약배양으로부터 녹색식물재생의 유의한 자극 효과가 있었다. 감마선과 관련한 약배양이 rice 품종을 개량하는 것이 복이배체 변이체의 높은 빈도는 효과적인 방법으로 사료된다.