<p>Hello to everyone </p><p>My ongoing experiment:</p><p>1. I am culturing <span style="font-weight: bold;">5-D5 rEGFL8</span> <em style="font-style: normal; color: rgb(0, 0, 0); font-family: arial, sans-serif; font-size:10pt; line-height: 16px; ">hibridomas cells for collecting sup. I have already collected about 600ml sup and which is already verified by western blot for positive signal of antibody. H</em><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px; ">opefully at the end of next week I will collect total 1 Liter sup. I have plane to use this sup for isolation of our desired mAB by using antibody purification kit and spin columns with PROSEP-G media.</span></p><p><br></p><p><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px;">2. Yesterday I have cultured E.coli for rEGFL8 protein isolation and today I proceeding the lysing step and solubilization in 8M urea. Tomorow I will determine their final protein conc. by using Nanodrop.After getting desired conc. I will proceed for dialysis (to refold rEGFL8 protein). Monday I will run on gel for purification analysis of my desired protein. </span></p><p><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px;"><br></span></p><p><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px;">3. I have plane to culture HUVEC cell, to study the effect of EGFL8 (inflammatory study) on Huvec cells. I need to order EGFM-2 basal medium for growing these cells but I dont know exactly that what type of media I should order. I search the site but I found a few type of EGM-2 media. </span></p><p><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px;"><br></span></p><p><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px; font-weight: bold;">Dear sir</span></p><p><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px;">Please let me know that what type of media I should order for culturing of HUVEC cells. </span></p><p><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size:10pt; line-height: 15px;">The previous one EGM-2 basal media (Lonza) containg VEGF,bFGF,IGF-1,EGF, Hydrocortisone and 2% FBS.</span></p>
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첫댓글교수님, EGM-2 배지가 기한이 넘어서 사용해야 할지 모르겠습니다. 파즐리가 아직 실험에 사용할 배지 등 상태에 대한 이해가 부족한 것 같아서 교수님의 확인 후 주문진행하겠습니다. Dear professor. We have only one EGF-2 medium and it's past their expiration date. Fazli doesn't completely understand what he needs. After you confirm, I order new medium and others.
첫댓글 교수님, EGM-2 배지가 기한이 넘어서 사용해야 할지 모르겠습니다. 파즐리가 아직 실험에 사용할 배지 등 상태에 대한 이해가 부족한 것 같아서 교수님의 확인 후 주문진행하겠습니다.
Dear professor. We have only one EGF-2 medium and it's past their expiration date. Fazli doesn't completely understand what he needs. After you confirm, I order new medium and others.
OK..