쌍황련 주사 분말의 항바이러스 효과 인플루엔자 A 바이러스 H5N1 시험관 내 및 생체 내

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https://pubmed.ncbi.nlm.nih.gov/29864534/

Antiviral effects of Shuanghuanglian injection powder against influenza A virus H5N1 in vitro and in vivo - PubMed

Antiviral effects of Shuanghuanglian injection powder against 

influenza A virus H5N1 in vitro and in vivo

쌍황련 주사 분말의 항바이러스 효과

인플루엔자 A 바이러스 H5N1 시험관 내 및 생체 내

Abstract; 요약하다;
현재 연구는 H5N1 바이러스 감염 후 시험관 내 및 생체 내에서 쌍황련(SHL) 주사 

분말의 보호 역할을 확인하는 것이었습니다. 면역 형광 염색을 사용하여 H5N1 

바이러스에 대한 쥐 장 점막 미세혈관 내피 세포(RIM-MVEC)의 감수성을 확인했습니다. 

RIM-MVEC의 바이러스 복제는 투과 전자 현미경(TEM), 적혈구 응집 분석 및 실시간 

정량 PCR로 측정했습니다. H5N1 바이러스에 감염된 RIM-MVEC 및 BALB/c 마우스를 

SHL로 처리하여 치료 효과를 조사했습니다. SHL 처리 후 동물 생존율과 

H5N1 바이러스에 감염된 BALB/c 마우스의 체중을 기록했으며, 마우스 폐에 적용된 

조직학 및 실시간 PCR을 사용하여 SHL의 항-H5N1 바이러스 효과를 확인했습니다. 

RIM-MVEC는 시험관 내에서 H5N1 바이러스의 복제를 지원했습니다. SHL 치료는 

H5N1 바이러스에 감염된 RIM-MVEC와 마우스 폐에서 바이러스 역가를 감소시켰습니다. 

SHL로 치료한 마우스는 대조군에 비해 생존했습니다. 감염 후 3일과 6일에 SHL로 

치료한 마우스의 폐에서 경미한 병리학적 변화, 감소된 염증 세포 침윤 및 더 적은 

바이러스 항원이 관찰되었습니다. 결론적으로, SHL은 바이러스 복제를 억제하고 

폐 손상을 완화함으로써 H5N1 바이러스 감염에 대한 항바이러스 활성을 가질 수 있습니다.

키워드: 항바이러스 효과; H5N1 인플루엔자 바이러스; 미세혈관 내피 세포; Shuanghuanglian.
저작권 © 2018 Elsevier Ltd. 모든 권리 보유.

Abstract

The current study was to identify a protective role of Shuanghuanglian (SHL) injection powder in vitro and in vivo after H5N1 viral infection. Immunofluorescent staining was used to determine the susceptibility of rat intestinal mucosa microvascular endothelial cells (RIM-MVECs) to the H5N1 virus. Viral replication of RIM-MVECs was measured by transmission electron microscopy (TEM) a hemagglutination assay and real-time quantitative PCR. H5N1 virally infected RIM-MVECs, and BALB/c mice were treated with SHL to investigate its therapeutic effect. Animal survival and the weight of H5N1 virally infected BALB/c mice after SHL treatment was noted, and histology and real-time PCR applied to mouse lungs were used to confirm‎ the anti-H5N1 viral effects of SHL. RIM-MVECs supported replication of the H5N1 virus in vitro. SHL treatment reduced viral titers in H5N1 virally infected RIM-MVECs and mouse lungs. SHL -treated mice survived compared to controls. Mild pathological changes, reduced inflammatory cell infiltration and fewer viral antigens were observed in the lungs of SHL-treated mice at days 3 and 6 post-infection. In conclusion, SHL may have the antiviral activity against the H5N1 virus infection by inhibiting viral replication and alleviating lung injury.

Keywords: Antiviral effect; H5N1 influenza virus; Microvascular endothelial cells; Shuanghuanglian.

Copyright © 2018 Elsevier Ltd. All rights reserved.

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