Re: 인체 장내 유익균은 크랜베리를 발효하여 어떤 성분으로 변화, 어떤 효과?... 2017

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Appl Environ Microbiol

. 2017 Aug 17;83(17):e01097-17. doi: 10.1128/AEM.01097-17

A Human Gut Commensal Ferments Cranberry Carbohydrates To Produce Formate

Ezgi Özcan a, Jiadong Sun b, David C Rowley b, David A Sela a,c,✉

Editor: Christopher A Elkinsd

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PMCID: PMC5561279  PMID: 28667113

ABSTRACT

Commensal bifidobacteria colonize the human gastrointestinal tract and catabolize glycans that are impervious to host digestion. Accordingly, Bifidobacterium longum typically secretes acetate and lactate as fermentative end products. This study tested the hypothesis that B. longum utilizes cranberry-derived xyloglucans in a strain-dependent manner. Interestingly, the B. longum strain that efficiently utilizes cranberry xyloglucans secretes 2.0 to 2.5 mol of acetate-lactate. The 1.5 acetate:lactate ratio theoretical yield obtained in hexose fermentations shifts during xyloglucan metabolism. Accordingly, this metabolic shift is characterized by increased acetate and formate production at the expense of lactate. α-l-Arabinofuranosidase, an arabinan endo-1,5-α-l-arabinosidase, and a β-xylosidase with a carbohydrate substrate-binding protein and carbohydrate ABC transporter membrane proteins are upregulated (>2-fold change), which suggests carbon flux through this catabolic pathway. Finally, syntrophic interactions occurred with strains that utilize carbohydrate products derived from initial degradation from heterologous bacteria.

초록

공생성 비피도박테리아는 인간 소화관에 정착하여 호스트의 소화 작용에 저항하는 글리칸을 분해합니다. 이에 따라 Bifidobacterium longum은 일반적으로 발효 최종 산물로 아세테이트와 락테이트를 분비합니다. 본 연구는 B. longum이 크랜베리 유래 xyloglucan을 균주 특이적으로 이용한다는 가설을 검증했습니다. 흥미롭게도, 크랜베리 xyloglucan을 효율적으로 이용하는 B. longum 균주는 2.0~2.5 몰의 아세테이트-젖산을 분비합니다. 육당 발효에서 얻은 1.5의 아세테이트:젖산 비율 이론적 수율은 xyloglucan 대사 과정에서 변화합니다. 이에 따라 이 대사 변화는 젖산 대신 아세테이트와 포르메이트 생산이 증가하는 특징을 보입니다. α-l-아라비노푸라노시다아제(아라비난 엔도-1,5-α-l-아라비노시다아제)와 탄수화물 기질 결합 단백질 및 탄수화물 ABC 운반체 막 단백질을 가진 β-xylosidase가 2배 이상 증가했습니다. 이는 이 분해 경로를 통해 탄소 유동이 발생함을 시사합니다. 마지막으로, 이질적 세균의 초기 분해에서 유래한 탄수화물 제품을 이용하는 균주와의 공생적 상호작용이 발생했습니다.

IMPORTANCE This was a study of bacterial metabolism of complex cranberry carbohydrates termed xyloglucans that are likely not digested prior to reaching the colon. This is significant, as bifidobacteria interact with this dietary compound to potentially impact human host health through energy and metabolite production by utilizing these substrates. Specific bacterial strains utilize cranberry xyloglucans as a nutritive source, indicating unknown mechanisms that are not universal in bifidobacteria. In addition, xyloglucan metabolism proceeds by using an alternative pathway that could lead to further research to investigate mechanisms underlying this interaction. Finally, we observed cross-feeding between bacteria in which one strain degrades the cranberry xyloglucan to make it available to a second strain. Similar nutritive strategies are known to occur within the gut. In aggregate, this study may lead to novel foods or supplements used to impact human health through rational manipulation of the human microbiome.

중요성 이 연구는 대장 도달 전에 소화되지 않을 가능성이 높은 복잡한 크랜베리 탄수화물인 xyloglucans의 세균 대사 과정을 조사했습니다. 이는 bifidobacteria가 이 식이 성분과 상호작용하여 이러한 기질을 활용해 에너지 및 대사물 생산을 통해 인간 호스트 건강에 영향을 미칠 수 있기 때문에 중요합니다. 특정 세균 균주는 크랜베리 xyloglucans를 영양원으로 활용하며, 이는 bifidobacteria에서 보편적이지 않은 미지의 메커니즘을 시사합니다. 또한, xyloglucan 대사는 대체 경로를 통해 진행되며, 이는 이 상호작용의 기전을 규명하기 위한 추가 연구로 이어질 수 있습니다. 마지막으로, 한 균주가 크랜베리 xyloglucan을 분해하여 두 번째 균주에 공급하는 교차 영양 공급을 관찰했습니다. 유사한 영양 전략은 장내에서도 알려져 있습니다. 종합적으로, 이 연구는 인간 미생물군집을 합리적으로 조작하여 인간 건강에 영향을 미치는 새로운 식품이나 보충제를 개발하는 데 기여할 수 있습니다.

KEYWORDS: bifidobacteria, food microbiology, prebiotics

INTRODUCTION

Microbial commensals colonize the mammalian gut and interact with their host through various interwoven metabolic networks. A well-characterized operation performed by microbiota is energy liberation from dietary polysaccharides. These complex carbohydrates are impervious to host digestion and are thus available for microbial populations to utilize. In turn, gut microorganisms produce metabolites sequestered by the host, including short-chain fatty acids (SCFAs) (1–4). Briefly, gut microorganisms compete for dietary molecules with metabolites secreted by one member often utilized by a secondary microbial population (5). This syntrophic metabolism is extended toward interactions with their host. For instance, microbial SCFAs (i.e., acetate, propionate, butyrate, and valerate) are substrates for specific host tissues. This includes butyrate, which provides a primary energy source for enterocytes (1, 2, 6).

Bifidobacterium longum is often dominant in the infant gut and colonizes adults at lower concentrations (7). Whereas B. longum subsp. infantis colonizes the infant gut, B. longum subsp. longum tends to populate adult microbiomes. As with all bifidobacteria, B. longum catabolizes carbohydrates via the fructose-6-phosphate phosphoketolase pathway, which is characteristic of the genus and thus termed the bifid shunt. This ATP-generating pathway results in acetate and lactate secretion to recycle cofactors required in substrate level phosphorylation (8, 9). The theoretical yield is an acetate:lactate ratio of 3:2 mol produced for every 2 mol of hexose that enters the bifid shunt. Bifidobacteria encode an assortment of glycosyl hydrolases (GHs) in order to utilize dietary glycans as fermentative substrates (10–12). Oligosaccharide utilization phenotypes are often consistent with the ecological niche that the bifidobacterial strain occupies (e.g., the adult versus the infant gut) (13). The B. longum subsp. longum genome, for example, encodes a large number of GHs dedicated to arabinose and xylose utilization (14). In contrast, the chromosome of the phylogenetic near neighbor and infant-colonizing bacterium B. longum subsp. infantis contains genes that enable human milk oligosaccharide utilization within the nursing infant gut (15–18). In general, B. longum subsp. infantis varies in its ability to utilize plant-derived carbohydrates (13).

Xyloglucans are cross-linking oligosaccharides found in type 1 plant cell walls (19) that exhibit a β(1→4)-glucan primary backbone with α(1→6)-linked xylosyl residues as substituents. Depending on the plant species and tissue of origin, xyloglucan branches may be extended by galactose, fucose, or arabinose residues (20, 21). The predominant cranberry xyloglucan structure was previously characterized as SSGG [S, β-d-glucose with α-l-Ara-(1, 2)-α-d-Xyl at the O-6 position; G, β(1→4)-glucan main chain] (22).

Oligosaccharides isolated from the cranberry (Vaccinium macrocarpon) cell wall prevent the adhesion of uropathogenic Escherichia coli and may limit biofilm production (23, 24). With regard to xyloglucan metabolism, certain gut microorganisms produce enzymes that degrade tamarind seed xyloglucans in their extracellular environment (25). However, the structure-function relationship between xyloglucans and specific populations of commensal bacteria remains unresolved. Thus, we eval‎uated B. longum strains for the ability to utilize cranberry-derived xyloglucans as a sole carbon source. To further understand in vitro metabolism, organic acids produced during fermentation were profiled. In addition, Lactobacillus strains that are recognized as probiotics (26) and have potential for use as synbiotics were subjected to growth on xyloglucans as their sole carbohydrate source.

KEYWORDS: bifidobacteria, 식품 미생물학, 프리바이오틱스

INTRODUCTION

미생물 공생체는 포유류 장에 정착하여 호스트와 다양한 상호 연결된 대사 네트워크를 통해 상호작용합니다. 미생물이 수행하는 잘 알려진 기능 중 하나는 식이 다당류로부터 에너지를 방출하는 것입니다. 이러한 복잡한 탄수화물은 호스트의 소화 작용에 저항하며, 따라서 미생물 군집이 이용할 수 있습니다. 반대로 장 미생물은 호스트에 의해 흡수되는 대사물을 생성하며, 이 중에는 단쇄 지방산(SCFAs)이 포함됩니다(1–4). 간단히 말해, 장 미생물은 식이 분자를 경쟁적으로 획득하며, 한 미생물이 분비한 대사산물이 다른 미생물 집단에 의해 활용되는 현상이 발생합니다(5). 이 공생적 대사 과정은 호스트와의 상호작용으로 확장됩니다. 예를 들어, 미생물 SCFAs(아세테이트, 프로피오네이트, 부티레이트, 발레레이트)는 특정 호스트 조직의 기질로 작용합니다. 이 중 부티레이트는 장 상피세포의 주요 에너지 공급원입니다(1, 2, 6).

Bifidobacterium longum은 영아 장내에서 우점종으로 존재하며 성인에서는 낮은 농도로 정착합니다(7).

B. longum subsp. infantis는 영아 장내에 정착하는

반면, B. longum subsp. longum은 성인 미생물군집에 주로 분포합니다.

모든 bifidobacteria와 마찬가지로 B. longum은 과당-6-인산 포스포케토라제 경로를 통해 탄수화물을 분해합니다. 이 경로는 속의 특징으로, bifid shunt라고 불립니다. 이 ATP 생성 경로는 기질 수준 인산화 과정에서 필요한 보조 인자를 재활용하기 위해 아세테이트와 락테이트를 분비합니다(8, 9). 이론적 수율은 비피드 션트에 들어가는 2몰의 헥소스마다 3:2 몰의 아세테이트:젖산 비율로 생성됩니다. 비피도박테리아는 식이 당을 발효 기질로 활용하기 위해 다양한 당수소화 효소(GH)를 암호화합니다 (10–12). 올리고당 이용 표현형은 종종 비피도박테리아 균주가 차지하는 생태적 틈새 (예: 성인 대 유아 장)와 일치합니다 (13). 예를 들어, B. longum subsp. longum 게놈은 아라비노스와 자일로스 이용에 특화된 다수의 GH를 암호화합니다 (14). 반면, 계통학적으로 가까운 이웃 종이자 영아 장내에 정착하는 B. longum subsp. infantis의 염색체에는 수유기 영아 장내에서 인간 모유 올리고당을 이용하는 데 필요한 유전자가 포함되어 있습니다(15–18). 일반적으로 B. longum subsp. infantis는 식물 유래 탄수화물 이용 능력에서 차이가 있습니다(13).

xyloglucan은 유형 1 식물 세포벽에 존재하는 교차 결합 올리고당으로(19), β(1→4)-glucan 주요 골격에 α(1→6)-xylosyl 잔기가 치환체로 결합되어 있습니다. 식물 종과 조직에 따라 xyloglucan 분지는 galactose, fucose, 또는 arabinose 잔기로 확장될 수 있습니다(20, 21). 크랜베리에서 주로 발견되는 xyloglucan 구조는 이전에 SSGG [S, β-d-glucose에 O-6 위치에 α-l-Ara-(1, 2)-α-d-Xyl이 결합된; G, β(1→4)-glucan 주요 사슬]로 특성화되었습니다(22).

크랜베리(Vaccinium macrocarpon) 세포벽에서 분리된 올리고사카라이드는 요로감염성 대장균의 부착을 방지하고 생물막 형성을 제한할 수 있습니다(23, 24). xyloglucan 대사 측면에서, 특정 장내 미생물은 세포 외 환경에서 타마린드 종자 xyloglucan을 분해하는 효소를 생성합니다 (25). 그러나 xyloglucan과 공생 세균의 특정 군집 간의 구조-기능 관계는 아직 명확히 규명되지 않았습니다. 따라서 우리는 B. longum 균주가 크랜베리 유래 xyloglucan을 유일한 탄소원으로 활용하는 능력을 평가했습니다. 체외 대사 과정을 더욱 이해하기 위해 발효 과정에서 생성된 유기산을 분석했습니다. 또한 프로바이오틱스로 인정받은 Lactobacillus 균주(26) 중 시너지틱스로 활용 가능성이 있는 균주를 xyloglucan을 유일한 탄수화물원으로 배양했습니다.

RESULTS

The cranberry cell wall contains xyloglucans.

Oligosaccharides were purified from a cranberry derivative through reverse-phase C18 and size exclusion chromatography (see Fig. S1 in the supplemental material). The chemical properties of the purified oligosaccharides were subsequently assessed by matrix-assisted laser desorption ionization (MALDI-TOF) mass spectrometry (MS) (Fig. 1) and 1H nuclear magnetic resonance (NMR) spectroscopy (Fig. S2). Consistent with previous studies (22, 24), the purified oligosaccharides were identified as xyloglucans with degrees of polymerization (DPs) ranging from 6 to 9. MALDI-TOF MS analysis identified sodium adduct ions at 923, 953, 1,055, 1,085, 1,217, 1,247, and 1,379, which were interpreted as xyloglucan compositions of hexose3-pentose3 (H3P3), H4P2, H3P4, H4P3, H4P4, H5P3, and H5P4, respectively. Putative cranberry xyloglucan oligosaccharide structures were provisionally assigned as reported in Table S1.

FIG 1.

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Purified cranberry xyloglucan putative structures. Shown is the positive reflectron mode MALDI-TOF MS spectrum of cranberry xyloglucan obtained from a HiPrep Sephacryl S-100 HR size exclusion column. The voltage was set at 80 kV, and 500 profiles were collected. Major peaks at m/z 923, 953, 1,055, 1,085, 1,217, 1,247, and 1,379 represent sodium adducts ([M + Na]+) of xyloglucan with DPs ranging from 6 to 9 and sugar compositions as follows: [H3P3+ Na]+, m/z 923; [H4P2+ Na]+, m/z 953; [H3P4+ Na]+, m/z 1,055; [H4P3+ Na]+, m/z 1,085; [H4P4+ Na]+, m/z 1,217; [H5P3+ Na]+, m/z 1,247; [H5P4+ Na]+, m/z 1,379.

B. longum xyloglucan utilization is strain dependent.

Among the bifidobacterial strains tested (Table 1), B. longum subsp. longum UCD401 exhibits the most growth on the purified xyloglucans, achieving a final optical density at 600 nm (OD600) of 0.15 ± 0.02 (Fig. 2). The growth rate of B. longum subsp. longum UCD401 while utilizing xyloglucans does not significantly differ from that during glucose utilization (P > 0.05) (Table 2). This indicates that B. longum subsp. longum UCD401 does not have a metabolic preference for glucose relative to xyloglucans. As expected, while fermenting xyloglucans, B. longum subsp. longum UCD401 achieves a modest biomass relative to that achieved on glucose (P < 0.05) and no growth was observed on the negative control. This indicates that UCD401 does not utilize these carbohydrates with similar efficiency. Bifidobacteria that utilize both plant and milk oligosaccharides have exhibited similar growth profiles (27, 28). Despite similar growth rates on glucose and xyloglucans, B. longum subsp. longum UCD401 ferments xyloglucans more slowly than glucose to achieve maximal growth (tc) (P < 0.05) (Table 2). In contrast to UCD401, B. longum subsp. infantis ATCC 15697 and JCM1260 did not metabolize cranberry xyloglucans.

TABLE 1.

Strains used in this study

StrainaSpecies or subspeciesOrigin

ATCC 15697	B. longum subsp. infantis	Human infant feces
JCM 1260	B. longum subsp. infantis	Human infant feces
JCM 1272	B. longum subsp. infantis	Human infant feces
JCM 7007	B. longum subsp. infantis	Human infant feces
JCM 11347	B. longum subsp. longum	Human feces
ATCC 15708	B. longum subsp. longum	Human feces
UCD401	B. longum subsp. longum	Human feces
ATCC BAA-793	L. plantarum	Human saliva
ATCC 33200	L. johnsonii	Human blood

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a

UCD, University of California Davis Culture Collection; ATCC, American Type Culture Collection; JCM, Japanese Collection of Microorganisms.

FIG 2.

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Bacterial growth while utilizing cranberry xyloglucans. Growth curves of B. longum subsp. longum UCD401, L. plantarum ATCC BAA-793, L. johnsonii ATCC 33200, and B. longum subsp. infantis ATCC 15697 and JCM 1260 grown on mMRS medium containing 2% (wt/vol) xyloglucans. The curves are drawn from an average of three independent experiments.

TABLE 2.

Analysis of bacterial growth kinetics calculated with Wolfram Mathematica 10.3

Strain2% xyloglucans2% glucosek (h−1)ΔODasymtc (h)k (h−1)ΔODasymtc (h)

B. infantis JCM 1260	NDa	ND	ND	ND	ND	ND
B. infantis ATCC 15697	ND	ND	ND	ND	ND	ND
B. longum UCD401	0.555 ± 0.055	0.15 ± 0.02b	19.7 ± 2.5d	0.614 ± 0.048	1.25 ± 0.09	13.7 ± 0.6
L. plantarum ATCC BAA-793	0.386 ± 0.049f	0.29 ± 0.03c	2.3 ± 0.60e	0.569 ± 0.005	1.48 ± 0.02	5.3 ± 0.60
L. johnsonii ATCC 33200	ND	ND	ND	ND	ND	ND

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a

ND, not determined.

b

Significant difference in the asymptotic OD value of B. longum UCD401 compared to L. plantarum and to the positive control, glucose (P < 0.05).

c

Significant difference in the asymptotic OD value of L. plantarum compared to B. longum UCD401 and to the positive control, glucose (P < 0.05).

d

Significant difference in the inflection point (tc) of B. longum UCD401 compared to L. plantarum and to the positive control (P < 0.05).

e

Significant difference in the inflection point (tc) of L. plantarum compared to B. longum UCD401 (P < 0.05).

f

Significant difference in the growth rate (k) of L. plantarum on xyloglucans compared to that of other strains on xyloglucans and glucose (P < 0.05).

Lactobacillus strains were eval‎uated to test if other fermentative bacteria could harness cranberry xyloglucan carbon. Lactobacillus plantarum ATCC BAA-793 and Lactobacillus johnsonii ATCC 33200 were subjected to growth on xyloglucans as a sole carbohydrate source as depicted in Fig. 2 with growth kinetics displayed in Table 2. L. johnsonii ATCC 33200 did not utilize cranberry-derived oligosaccharides. In contrast, L. plantarum ATCC BAA-793 achieved a final OD600 of 0.29 ± 0.03, which is higher than that of all of the B. longum strains tested (P < 0.05) (Fig. 2). This Lactobacillus strain does prefer glucose, as it has a significantly lower growth rate while consuming xyloglucans (P < 0.05, Table 2). Its maximal growth (tc) did not vary between xyloglucan and glucose utilization (P > 0.05), despite the lower growth rate during xyloglucan utilization. Interestingly, L. plantarum has a lower growth rate than B. longum subsp. longum UCD401 while fermenting purified xyloglucans despite achieving a higher biomass (P < 0.05). The time required to achieve maximum growth (tc) while utilizing xyloglucan is significantly shorter for L. plantarum than for B. longum subsp. longum UCD401 (P < 0.05). This suggests that L. plantarum does not prefer these complex carbohydrates, despite having a higher utilization efficiency than the B. longum strain.

B. longum consumes xyloglucans from the growth medium.

The extent to which UCD401 utilizes xyloglucans was determined by profiling spent fermentation medium from growth conducted in microcentrifuge tubes. Interestingly, degraded glycans were not detected in spent medium compared with the control (i.e., preinoculated medium) (Fig. S3 and S4). The extracted ion counts for each xyloglucan were quantitated as percentages of the baseline control (t = 0) (Fig. S5 and S6). Of interest, detectable xyloglucans with DPs of 6 to 9 exhibited >120% of the extracted ion counts of the baseline (t = 0). Thus, there was an extensive accumulation of xyloglucans with all DPs following fermentation by B. longum subsp. longum UCD401. Significantly, xyloglucans with DPs of <7 did not accumulate to the same extent. This is indicative of an ability to utilize the short-chain oligosaccharides. Accordingly, we did not detect monosaccharide accumulation in the spent fermentation medium. A similar observation has been reported previously with bifidobacterial metabolism of human milk oligosaccharides (29, 30). As previously postulated, B. longum is able to transport only lower-molecular-weight oligosaccharides across its envelope. This suggests that a dynamic equilibrium has been achieved between the import of lower-DP molecules and the extracellular hydrolysis of higher-DP molecules. This is consistent with higher-DP oligosaccharide accumulation in the growth medium. A similar glycan distribution was observed in L. plantarum qualitatively, along with corresponding degradation phenotypes (Fig. S3). It is important to note that glycoprofiling was performed with bacteria grown in microcentrifuge tubes.

Bifidobacteria metabolize xyloglucans via the bifid shunt.

Metabolite end products secreted while utilizing xyloglucans were quantified by high-performance liquid chromatography (HPLC) of cell-free supernatants. The absolute concentrations of acetate, lactate, and formate secreted by B. longum subsp. longum UCD401 in the early stationary phase of xyloglucan or glucose utilization are depicted in Fig. 3. B. longum subsp. longum UCD401 secretes significantly lower lactate and acetate concentrations while utilizing xyloglucans (4.08 ± 0.16 and 8.78 ± 0.10 mM, respectively) than when subsisting on glucose (37.60 ± 9.41 and 54.11 ± 13.40 mM, respectively). This is expected and consistent with the lower biomass achieved (P < 0.05). In addition, less metabolic investment is required to catabolize glucose in the bifid shunt. The absolute concentrations were normalized with respect to the OD600 reached in microcentrifuge tubes. UCD401 exhibited higher acetate concentrations in xyloglucan metabolism (172.54 ± 10.52 mM) than in glucose metabolism (124.56 ± 22.70 mM), whereas the lactate concentrations were similar (80.31 ± 6.76 and 86.50 ± 15.98 mM). Interestingly, and despite a lower biomass, B. longum subsp. longum UCD401 secretes high concentrations of formate while metabolizing cranberry xyloglucans (10.01 ± 0.86 mM). This is significantly higher than during glucose utilization (1.62 ± 1.30 mM) (P < 0.05), indicating a shift in metabolism to harvest energy more efficiently from this substrate.

FIG 3.

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Bacterial fermentative end products of cranberry xyloglucan utilization. Shown are lactate (a), acetate (b), and formate (c) production; acetate/lactate ratios (d); and formate/lactate ratios (e). Bl, B. longum subsp. longum UCD401; Lp, Lactobacillus plantarum ATCC BAA-793. Averages of independent biological triplicates are shown, and bars represent the standard deviation of the mean. Organic acid production is expressed in millimolar absolute concentrations. Asterisks represent significant differences determined by two-way ANOVA and Sidak's multiple-comparison test (P < 0.05).

The bifid shunt catabolizes hexose sugars to yield a theoretical acetate:lactate ratio of 1.5. As expected, UCD401 achieved this while utilizing glucose (1.44 ± 0.01); however, xyloglucan fermentation shifted the ratio significantly toward acetate at the expense of lactate (2.15 ± 0.08) (P < 0.05) (Fig. 3d). When normalizing the absolute concentrations with respect to biomass, more acetate production from xyloglucan metabolism than from glucose metabolism occurred. This has been observed in a previous study, as more ATP is produced by flux through acetate-producing pathways (31). Accordingly, formate secretion was significantly increased with a formate:lactate ratio of 2.50 ± 0.28 while fermenting xyloglucans, in contrast to 0.05 ± 0.01 during glucose metabolism (Fig. 3e).

Organic acids produced while lactobacilli ferment xyloglucans were subsequently profiled (Fig. 3). In addition to comparative physiology, this approach may inform future mixed-culture probiotics (i.e., lactobacilli and bifidobacteria), as well as synbiotic strategies that incorporate xyloglucans with one or more probiotic strain. Lactate was produced at significantly lower concentrations (5.27 ± 0.19 mM, P < 0.05) by L. plantarum ATCC BAA-793 than during glucose consumption. In addition, acetate secretion was observed with an acetate:lactate ratio of 1:1 when L. plantarum ATCC BAA-793 utilized cranberry xyloglucans (Fig. 3d). While fermenting glucose, L. plantarum exhibited an acetate:lactate ratio of 0.05 ± 0.01 (P < 0.05) (Fig. 3d). We expected L. plantarum, as a heterofermentative species, to secrete acetate. Interestingly, formate production was detected during xyloglucan consumption, although it was not detected following glucose fermentation. The secretion of acetate and formate has been previously observed when inefficient substrate utilization yielded less biomass (32, 33).

Bacterial commensals exhibit differential growth phenotypes while consuming oligosaccharide extracts containing secondary plant material.

In addition to purified xyloglucans, crude cranberry cell wall extracts were eval‎uated as a growth substrate. These fractions are more crudely enriched for oligosaccharides and thus more closely mimic what would be encountered by the microbiota following the ingestion of cranberries. Moreover, prebiotic formulations would likely use a crude cranberry extract for functional or processing reasons.

We tested two crude fractions, termed A2, which retains a pink color and is extracted from whole cranberries, and A6, which is derived from the primary A2 fraction. Both of these preparations have been previously reported (22). However, the precise distribution of glycans and noncarbohydrate molecules in A2 is not fully characterized. Accordingly, B. longum strains achieved more pronounced growth phenotypes while consuming A2 and A6. Cellular growth (Fig. 4) and kinetics varied by the particular strain tested (Table 3). A2 was most vigorously utilized by B. longum subsp. infantis JCM7007 (OD600 of 0.58 ± 0.34), B. longum subsp. infantis JCM1272 (OD600 of 0.38 ± 0.10), and B. longum subsp. longum UCD401 (0.37 ± 0.07) (Fig. 4a; Table 3). With the exception of UCD401, these strains did not grow on purified xyloglucans. Interestingly, there are two B. longum subsp. infantis strains that utilize A2 as a substrate. This is significant, as B. longum subsp. infantis is predicted not to utilize xyloglucans; thus, other components of the A2 fraction were fermented or cofermented. In addition, B. longum subsp. infantis JCM1272 achieved moderate growth on A2 (OD600 of 0.17 ± 0.08), whereas growth on purified xyloglucans was not observed.

FIG 4.

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Bacterial growth on additional fractions of cranberry xyloglucans. Growth curves represent B. longum subsp. infantis ATCC 15697, JCM 1260, JCM 1272, and JCM 7007; B. longum subsp. longum JCM 11347, ATCC 15708, and UCD401; Lactobacillus plantarum ATCC BAA-793; and Lactobacillus johnsonii ATCC 33200 grown on mMRS medium containing 2% (wt/vol) xyloglucan fraction A2 (a) or 2% (wt/vol) xyloglucan fraction A6 (b). The curves are drawn from the average of at least three independent experiments, with the exception of strains JCM 1260, JCM 7007, JCM 11347, and JCM 15708 which are based on duplicates in panel a.

TABLE 3.

Kinetic analysis of bacterial growth on A2 and A6 fraction xyloglucans calculated with Wolfram Mathematica 10.3

StrainXyloglucans from fraction:A2A6k (h−1)ΔODasymtc (h)k (h−1)ΔODasymatc (h)

B. infantis JCM 1260	0.464 ± 0.280	0.17 ± 0.08	6.4 ± 3.3	1.188 ± 0.79	0.08 ± 0.02a	3.3 ± 1.8
B. infantis JCM 1272	0.427 ± 0.039	0.38 ± 0.10	12.4 ± 0.6	NDc	ND	ND
B. infantis JCM 7007	0.215 ± 0.137	0.58 ± 0.34	17.6 ± 6.1	ND	ND	ND
B. infantis ATCC 15697	NAd	NA	NA	ND	ND	ND
B. longum JCM 11347	ND	ND	ND	ND	ND	ND
B. longum ATCC 15708	ND	ND	ND	ND	ND	ND
B. longum UCD401	0.438 ± 0.152	0.37 ± 0.08	11.3 ± 3.0	0.270 ± 0.084	0.27 ± 0.03b	16.3 ± 0.6
L. plantarum ATCC BAA-793	NA	NA	NA	0.513 ± 0.061	0.25 ± 0.02b	2.9 ± 0.5
L. johnsonii ATCC 33200	NA	NA	NA	0.944 ± 0.166	0.12 ± 0.01a	1.4 ± 0.3

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a

Significant difference in the asymptotic OD values for JCM 1260 and L. johnsonii compared to UCD401 and L. plantarum in A6 fraction treatment (P < 0.05).

b

Significant difference in the asymptotic OD values for UCD401 and L. plantarum compared to JCM 1260 and L. johnsonii in A6 fraction treatment (P < 0.05).

c

ND, not determined.

d

NA, not available.

In contrast, the more purified A6 fraction was utilized only by B. longum subsp. longum UCD401 and B. longum subsp. infantis JCM 1260, with final OD600s of 0.27 ± 0.03 and 0.08 ± 0.02, respectively (Fig. 4b). Clearly, the A6 fraction is not as efficiently utilized as the cruder and likely polyphenol-containing A2 fraction (Table 3; Fig. S7). UCD401 exhibited a gradual decline in growth efficiency while utilizing the crudest extract to the most purified form (Fig. S7b) (P < 0.05). This mirrors the general trend that the majority of bifidobacterial strains tested do not metabolize highly purified xyloglucans. This includes all B. longum subsp. infantis strains (Fig. S7c).

B. longum subsp. longum UCD401 utilizes A6, leading to an increase in the acetate:lactate ratio (1.90 ± 0.16) and absolute formate production (8.87 ± 2.63 mM) relative to those achieved with glucose, despite the lower biomass (P < 0.05) (Fig. 5). Formate production by UCD401 is greater than that by B. longum subsp. infantis JCM1260 when catabolizing A6 (P < 0.05). This is consistent with formate secretion while utilizing highly purified xyloglucans and suggests that UCD401 is specifically consuming this substrate in the mixed-purity preparation. Interestingly, B. longum subsp. infantis JCM1260 exhibits an acetate:lactate ratio of 2.44 ± 0.32 and 6.39 ± 0.57 mM formate secreted (Fig. 5c). Glucose flux through the bifid shunt was metabolized, as expected, to approximate the theoretical yield of 1.5 (1.82 ± 0.15 and 1.43 ± 0.01) (P < 0.05) (Fig. 5d). Again, this is consistent with utilization of the carbohydrate constituents of the A6 fraction.

FIG 5.

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Bifidobacterial fermentative end products of cranberry fraction A6 utilization. (a to c) Lactate (a), acetate (b), and formate (c) production while B. longum subsp. infantis JCM 1260 and B. longum subsp. longum UCD401 utilize xyloglucan fraction A6. (d) Acetate:lactate ratio after fermentation. Averages of independent biological triplicates are shown, and bars represent the standard deviation of the mean. Asterisks represent significant differences eval‎uated by two-way ANOVA and Sidak's multiple-comparison test (P < 0.05).

Expression‎ of arabinose utilization genes while utilizing the A6 cranberry xyloglucan fraction.

The expression‎ of four GH family genes in the UCD401 chromosome while metabolizing A6 was eval‎uated. This includes an α-l-arabinofuranosidase gene (BL_0405), two arabinan endo-1,5-α-l-arabinosidase genes (BL_0404, BL_0403), and a β-xylosidase gene (BL_0402). We observed that BL_0405, BL_0404, and BL_0402 were significantly upregulated, with a minimum of a 2-fold increase during exponential phase (Fig. 6a, P < 0.05). This indicates that the expression‎ of these genes and their products is modulated during the utilization of the A6 xyloglucan fraction. Interestingly, BL_0403 expression‎ did not significantly change relative to that of the glucose control (P > 0.05). This indicates that the adjacent BL_0404 gene is responsible for xyloglucan hydrolysis. Moreover, differential regulation of these paralogs potentially reflects divergent enzymatic functions.

FIG 6.

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Gene expression‎ of B. longum subsp. longum UCD401 while utilizing cranberry fraction A6 as a sole carbon source. Genes in the arabinose cluster of B. longum subsp. longum UCD401 predicted to participate in xyloglucan metabolism are depicted on the x axis. Expression‎ of four GH family genes (those for α-l-arabinofuranosidase, C-terminal [BL_0405], arabinan endo-1,5-α-l-arabinosidase [BL_0404, BL_0403], β-xylosidase [BL_0402]) (a) and those for a carbohydrate ABC transporter substrate-binding protein (BL_0398) and carbohydrate ABC transporter membrane proteins (BL_0397, BL_0396) (b) is shown as fold change relative to the control (glucose). Averages of independent biological triplicates are shown, and bars represent the standard deviation of the mean. Asterisks (* and **) represent significant differences compared to the control (glucose), eval‎uated by paired t test (P values of <0.05 and <0.005, respectively).

Fermentation of the A6 fraction induces UCD401 to express transport-related proteins that may be involved in xyloglucan transport across the membrane. Upregulated transport genes, including those for a carbohydrate ABC transporter substrate-binding protein (BL_0398) and carbohydrate ABC transporter membrane proteins (BL_0397, BL_0396) exhibit >2-fold induction relative to the control (Fig. 6b, P < 0.05). This suggests recognition and a potential uptake of arabinose and xylose backbone motifs across the cell envelope.

Modeled bidirectional syntrophic interactions.

Conditioned spent medium from xyloglucan fermentations was used to assess in vitro bidirectional syntrophic interactions in microplate growth. This occurs when fermentation by a primary strain provides hydrolysis products to be used by a heterologous secondary strain that is incapable of utilizing the initial substrate. Since L. johnsonii does not grow on xyloglucans on a microplate, we included this strain as a negative control. Interestingly, B. longum subsp. infantis ATCC 15697 does not utilize purified xyloglucans as a sole carbon source; however, conditioned supernatants from L. plantarum and L. johnsonii enabled growth to final OD600s of 0.066 ± 0.008 and 0.093 ± 0.008 with growth rates of 0.940 ± 0.106 h−1 and 0.801 ± 0.077 h−1, respectively (Table 4). We interpret this as moderate growth because of the characteristic sigmoidal response curve produced during fermentation. In contrast, supernatant harvested from B. longum subsp. longum UCD401 did not enable B. longum subsp. infantis ATCC 15697 to grow (Fig. 7). This is likely due to the inability of B. longum subsp. infantis ATCC 15697 to utilize arabinosyl-reducing ends, as predicted by comparative genomics (15). UCD401 may potentially sequester and metabolize molecules that would otherwise be utilized by other bifidobacterial strains. Furthermore, it is possible that the lactobacilli secreted other products to enhance B. longum subsp. infantis growth (e.g., exopolysaccharides) (34).

TABLE 4.

Growth kinetics of strains during syntrophic interaction

StrainSupernatant from:B. longum UCD401L. plantarum ATCC BAA-793L. johnsonii ATCC 33200k (h−1)ΔODasymtc (h)k (h−1)ΔODasymtc (h)k (h−1)ΔODasymtc (h)

B. infantis ATCC 15697	NDe	ND	ND	0.940 ± 0.106a	0.066 ± 0.008	10.3 ± 0.6	0.801 ± 0.077	0.093 ± 0.008	10.3 ± 0.6
B. longum UCD401	NAf	NA	NA	0.632 ± 0.120b	0.096 ± 0.005d	12.8 ± 0.6	0.468 ± 0.070d	0.162 ± 0.007c	11.0 ± 0.0
L. plantarum ATCC BAA-793	0.890 ± 0.158d	0.115 ± 0.009d	2.1 ± 0.9	NA	NA	NA	NA	NA	NA

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a

Significant difference between the growth rates of B. infantis ATCC 15697 in different conditioned media determined by paired t test (P < 0.05).

b

Significant difference between the growth rates of B. longum UCD401 in different conditioned media determined by paired t test (P < 0.05).

c

Significant difference between the asymptotic OD values of B. longum UCD401 in different conditioned media determined by paired t test (P < 0.05).

d

Significant difference in the growth kinetics of each strain in different conditioned media and its growth kinetics in xyloglucans alone (shown in Table 2) determined by paired t test (P < 0.05).

e

ND, not determined.

f

NA, not available.

FIG 7.

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Bacterial syntrophic interactions modeled with cranberry xyloglucans. Growth curves of B. longum subsp. infantis ATCC 15697 on mMRS medium containing xyloglucans (green) and supernatants from B. longum subsp. longum UCD401 (orange), L. plantarum ATCC BAA-793 (blue), and L. johnsonii ATCC 33200 (yellow) after xyloglucan fermentation. The curves are drawn from an average of three independent experiments.

B. longum subsp. infantis ATCC 15697 utilization of carbohydrates liberated from lactobacilli and UCD401 primary fermentations were analyzed by liquid chromatography (LC)-MS (Fig. S8). Of interest, ATCC 15697 grew on the spent medium from lactobacillus primary fermentations, albeit with a stronger peak intensity observed by LC-MS. This may be due to hydrolysis of higher-molecular-weight xyloglucans or the production of exopolysaccharides, as hypothesized with primary fermentations. In addition, we determined that ATCC 15697 is incapable of utilizing the spent medium from UCD401 in a secondary fermentation.

When B. longum subsp. longum UCD401 was grown on the L. plantarum supernatant, it achieved a lower final OD600 (0.096 ± 0.005) than when it was grown on purified xyloglucans in a primary fermentation (P < 0.05), although the growth rates are similar regardless of whether UCD401 is conducting a primary or a secondary fermentation (Table 4). Conversely, the growth rate of L. plantarum on conditioned medium from B. longum subsp. longum UCD401 was significantly higher whereas its biomass production (final OD600 of 0.115 ± 0.009) was significantly lower than that of purified xyloglucans (final OD600 of 0.29 ± 0.03) (P < 0.05) (Table 4). This may indicate the production of inhibitory compounds or a preference for intact oligosaccharides.

DISCUSSION

It has been established that genotypic and phenotypic variations contribute to bifidobacterial preferences for carbon sources (12). For the former, the B. longum subsp. infantis genome has evolved to enable milk oligosaccharide utilization at the expense of plant carbohydrates. Bifidobacteria deploy transporters to capture intact oligosaccharides or their derivatives from the extracellular milieu (13, 35). Thus, the inability of B. longum subsp. infantis strains to utilize xyloglucans may be due to the absence of specific transporters. This is consistent with B. longum subsp. infantis ATCC 15697 utilization of lactobacillus hydrolysis products. These lactobacilli perform extracellular digestion to provide ATCC 15967 with fermentable substrates. In contrast, UCD401 internalizes intact oligosaccharides to withhold potential growth substrates from ATCC 15697.

It is likely that B. longum subsp. longum UCD401 utilizes xyloglucans from the terminal arabinose positioned at the reducing end. This is consistent with the expression‎ of arabinose utilization genes (Fig. S9). This arabinose utilization cluster is conserved in B. longum subsp. longum strains with an araC transcriptional family operon upstream of α-l-arabinofuranosidase and β-xylosidase genes adjacent to an ABC transporter cassette. Importantly, B. longum subsp. longum ATCC 15707 does not grow on xylose but metabolizes arabinose as a sole carbohydrate source (36). Accordingly, B. longum subsp. longum UCD401 utilizes arabinose more efficiently than do the other B. longum subsp. longum strains tested (Fig. S10). It is noteworthy that this B. longum strain was isolated from an infant stool sample and the stronger utilization phenotype may reflect recent isolation and limited passages.

Bifidobacterial xyloglucan utilization requires the transport of arabinose following extracellular hydrolysis of higher-DP oligosaccharides. β-d-Glucopyranosyl and α-d-xylopyranosyl residues are not metabolized extracellularly. This likely causes the accumulation of lower-DP xyloglucans. Arabinose metabolism has been linked to genes encoding extracellularly secreted α-arabinofuranosidases, which are located within the UCD401 genome (Fig. S4) (37). As we predicted, B. longum subsp. longum UCD401 utilizes xyloglucan within the crude A6 fraction likely by using an extracellular arabinofuranosidase (BL_0405). Arabinose liberated from xyloglucans was completely sequestered, as it was not detected in spent medium. It has been posited that bifidobacterial strains cannot use xylose backbones longer than xylotetraose (DP of 4) (37). The B. longum subsp. longum arabinose cluster encodes predicted intracellular xylosidases, which supports the hypothesis that transport is essential for the catabolism of higher (DP of >4) xylooligosaccharides (Fig. S4). The upregulation of solute-binding transport proteins (BL_0398) and permeases (BL_0397 and BL_0396) suggests that uptake of arabinose and lower-DP xyloglucans, including xylose and glycosyl residues, occurs after the release of arabinose moieties.

In bifidobacterial carbohydrate metabolism, pyruvate is converted to lactate via lactate dehydrogenase, which does not produce additional ATP but recycles NAD+ (38). Pyruvate formate-lyase (pfl; EC 2.3.1.54) catalyzes formate and acetyl-coenzyme A (acetyl-CoA) production from pyruvate. Acetyl-CoA is subsequently metabolized to acetate or ethanol and secreted (38). This results in an increased acetate/lactate ratio and correspondingly higher ATP production (31, 38). We predicted increased acetate secretion during UCD401 xyloglucan fermentation, although the lower biomass on this substrate hinders comparisons of absolute concentrations. However, formate and ethanol secretion was previously determined to impact the acetate/lactate ratio in bifidobacteria (38). Thus, the shift toward formate production coincides with an increased need for ATP while metabolizing xyloglucans. Bifidobacterial secretion of formate while utilizing certain oligosaccharides has been previously observed (39–41). Under certain conditions, bifidobacterial oligosaccharide metabolism is initially characterized by high levels of lactate production, with a shift toward formate production occurring later in the fermentation (40, 42, 43). This suggests that formate production benefits the cell by generating additional ATP when oligosaccharides are catabolized slowly. In general, bifidobacteria secrete secondary products such as formate, succinate, and ethanol when achieving lower biomass concentrations (OD600 of <0.5) and when the carbohydrate consumption rate is diminished (31, 39, 40, 42). Our results are consistent in that formate production increases while xyloglucans are utilized slowly, ostensibly to bolster ATP production under these conditions.

Crude oligosaccharide extracts were tested, as they is of interest for the preparation of cranberry xyloglucans for prebiotic applications. The cranberry cell wall does not contain appreciable quantities of monosaccharides (44) and is not expected to influence growth (22). As the A2 fraction retains a pink color, there are likely small quantities of phenolic compounds that may contribute to the observed growth differential between the A2 fraction and the highly purified xyloglucans. Furthermore, the most abundant oligosaccharides within the A6 fraction are identical to the xyloglucans purified in this study (22). However, XSGGG-Ac and XSGGG-Ac2 structures (i.e., acetylated xyloglucan oligosaccharides) have been identified in the A6 fractions and did not remain in the highly purified xyloglucans. Thus, these oligosaccharides may be responsible for promoting L. johnsonii and B. longum subsp. infantis JCM1260 growth. B. longum typically consumes short-chain oligosaccharides, likely subsequent to intracellular transport (13, 45). Thus, it is possible that differential phenotypes are due to variation in the xyloglucan DP.

Lactobacilli are natural inhabitants of the human gastrointestinal tract and may be used as probiotics and in concert with prebiotics in synbiotic applications (26, 46). Lactobacillus strains may utilize carbohydrates as well as bifidobacteria (47). It is likely that L. plantarum uses the phosphoketolase pathway while consuming xyloglucans to secrete formate and acetate, with lactate being reduced (48, 49). This was previously observed in L. plantarum VTT E-79098 fermentation of arabino-xylooligosaccharide (33).

Our experiments suggest that syntrophic interactions following xyloglucan degradation may occur between other members of the gut consortium. The interactions among lactobacilli, bifidobacteria, and oligosaccharides are of interest in the engineering of synbiotic interventions. Moreover, lactobacillus-mediated cleavage of xyloglucans within the small intestine would impact substrates available to bifidobacterial commensals of the lower gastrointestinal tract. Syntrophic interactions during bifidobacterial in vitro cofermentations that include members of the genus Bifidobacterium on various carbohydrate sources have been previously described (12, 45, 50). Cooperative or cross-feeding between strains that colonize the gastrointestinal tract may contribute to the competitive exclusion of sensitive taxa and maintain diversity through specialist adaptation to colonize unique niches (51). Such syntrophic interactions under in vivo conditions might prompt a shift in the gut microbiome toward the metabolism of plant-derived carbohydrates with a metabolome characteristic of saccharolytic activities (52). We did not detect monosaccharides in spent medium by HPLC, leading to the hypothesis that liberated monosaccharides would be sequestered, thus rendering them undetectable. Furthermore, it is possible that the transfer of conditioned medium from the primary fermentation introduced end products into the secondary fermentation although at very low concentrations with minimal impact on growth.

Conclusions.

Among the bifidobacterial strains tested, B. longum subsp. longum UCD401 utilizes cranberry xyloglucans as a sole energy and carbon source. This is potentially significant, as it was recently isolated from an infant and subjected to limited passages. This may be due to conserved phenotypic function, as genomic or regulatory features likely remained intact in the limited generations since its isolation (53). Furthermore, it hints at the potential for strain variation within infants and adults in the capacity to utilize cranberry xyloglucans. B. longum subsp. longum colonizes both infants and postweaning individuals. The phenotypic versatility underlying the broad host range necessary to subsist on a substrate encountered in the adult diet suggests a postweaning strategy. It is notable that xyloglucan catabolism prompts a shift in the central fermentative pathway to obtain more ATP. The significance of the altered metabolic profile for microbiome function or host health remains an outstanding question.

MATERIALS AND METHODSIsolation of xyloglucans from the cranberry cell wall.

Cranberry hulls were degraded with pectinase (Klerzyme 150; DSM Food Specialties, South Bend, IN, USA) and fractionated as previously described (24), with modifications. Briefly, 2 g of cranberry pectinase-treated powder was dissolved in 20 ml of distilled water and loaded onto a RediSep GOLD C18 reverse-phase column (Teledyne ISCO, Inc., Lincoln, NE, USA) connected to a CombiFlash Rf purification system (Teledyne ISCO, Inc.) The column was eluted sequentially with 500 ml of deionized water, 500 ml of 15% methanol-water, and 500 ml of methanol. Fractions from all of the gradients were individually pooled and lyophilized to obtain three major fractions, Cranf1W (761 mg, 38.1%) eluted with 100% deionized water, Cranf1b (476 mg, 23.8%) eluted with 15% methanol-water, and Cranf1M (562 mg, 28.1%) eluted with 100% methanol. A 100-mg sample of Cranf1b was then dissolved in 2 ml of distilled water and further purified with a size exclusion column HiPrep Sephacryl S-100 HR 16/60 (GE Healthcare Life Sciences, Pittsburgh, PA, USA). The column was isocratically eluted with deionized water at 0.5 ml/min. Eluates were collected from every 5-ml volume and eval‎uated for their total carbohydrate content by phenol sulfuric acid assay (54). The total carbohydrate content was assessed with a 96-well microtiter plate as previously reported (54). Briefly, in each well of a 96-well microtiter plate, 30 μl of each fraction was mixed with 100 μl of concentrated sulfuric acid and 20 μl of 5% phenol solution. The microtiter plate was than incubated at 90°C for 5 min, and the absorbance at 490 nm was recorded with a SpectraMax M2 microplate reader (Molecular Devices, Sunnyvale, CA, USA).

Xyloglucan structural analysis.

Oligosaccharide fractions were pooled and freeze-dried to obtain 59.5 mg of cranberry xyloglucans, which was then chemically verified by a combination of MALDI-TOF MS and 1H NMR spectroscopy. Briefly, 1 μl of xyloglucan (1 mg/ml in H2O) was mixed with 1 μl of 2,3-dihydrobenzoic acid matrix solution. A 2-μl volume of this mixture was analyzed by MALDI-TOF MS (Axima Performance, Shimadzu, Kyoto, Japan) in positive reflectron mode. Five hundred profiles were collected for each experiment. Furthermore, the cranberry xyloglucans were dissolved in D2O (99.96%; Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA). The 1H NMR spectrum was obtained on a 500-MHz NMR spectrometer (Varian VNMR; Agilent Technologies, Santa Clara, CA, USA) at 25°C.

Bacterial strains and propagation.

The bacterial strains used in this study are summarized in Table 1. Bifidobacterial strains were propagated in bifidobacterial selective medium or De Man-Rogosa-Sharpe (MRS; Oxoid, Hampshire, England) medium supplemented with 0.05% (wt/vol) l-cysteine (Sigma-Aldrich, St. Louis, MO) (55) at 37°C under anaerobic conditions (Coy Laboratory Products, Grass Lake, MI). Lactobacillus cultures were propagated in MRS medium supplemented with 0.05% (wt/vol) l-cysteine as a reducing agent at 37°C under anaerobic conditions. Bacterial strains were routinely verified with the bifidobacterium-specific phosphoketolase assay (56) and through microscopy. The strains used in this study have been previously confirmed by multilocus sequence typing and urease assay to distinguish B. infantis and B. longum (urease+ and urease−) (16). In addition, a PCR-based B. longum/B. infantis ratio analysis was performed to differentiate B. longum subspecies as previously described (57).

Microplate growth assay.

To eval‎uate growth phenotypes in a 96-well format, overnight cultures were centrifuged and washed with phosphate buffer solution that was inoculated (1% [vol/vol]) into modified MRS (mMRS) medium (without acetate and carbohydrate substrate). The sole carbon source was defined as purified xyloglucans at a final concentration of 2% (wt/vol). The growth assay was conducted anaerobically at 37°C for 72 h by assessing OD600 with an automated PowerWave HT microplate spectrophotometer (BioTek Instruments, Inc., Winooski, VT). Each strain was eval‎uated in biological triplicate with three technical replicates. Negative and positive controls consisted of inoculated medium in the absence of substrate and the presence of glucose (2% [wt/vol]), respectively. Bacterial growth kinetics were calculated with Wolfram Mathematica 10.3 Student Edition as described by Dai et al. (58) in accordance with the equation ΔOD(t) = ΔODasym{[1/1 + exp(ktc − t)] − [1/1 + exp(ktc)]}, where ΔODasym is the growth level at stationary phase with k representing the growth rate and tc is the inflection point indicating the time required to reach the highest growth rate.

Modeled syntrophic interactions.

To determine if degradative or secreted metabolites are utilized by a heterologous strain, supernatants from microplate-grown bacteria were analyzed. Accordingly, 800 μl of the conditioned supernatant was dissolved in 1,200 μl of mMRS medium. Bacterial strains were washed once with phosphate-buffered saline to remove the residual carbohydrates from the initial propagation, inoculated at 1% (vol/vol), and eval‎uated for growth in a 96-well format as already described. Bacterial growth kinetics were calculated, and the supernatant obtained after the secondary growth was analyzed by HPLC.

Xyloglucan profiling following bacterial fermentation.

Following in vitro fermentation, filtered bacterial culture supernatants were derivatized with 2-aminobenzamide (2-AB) for oligosaccharide purification. Briefly, 50 μl of cell-free supernatant was diluted with 2 ml of deionized water and loaded onto a porous graphitized carbon cartridge (1 g; Thermo Scientific, Waltham, MA, USA). The cartridge was prewashed with 5 ml of 50% (vol/vol) acetonitrile-H2O and equilibrated with 3 × 5 ml of deionized water. First, the cartridges were eluted with 5 ml of deionized water three times with oligosaccharide fractions eluted with 30% (vol/vol) acetonitrile-H2O with 0.1% trifluoroacetic acid (TFA) three times. The resulting fractions were spiked with 0.036 mg of glucose as an internal standard and freeze-dried. 2-AB at 7 mg/ml and 2-picoline borane at 3.2 mg/ml were prepared in 10% (vol/vol) acetic acid-H2O, and 200 μl was added to each mixture. The solutions were held at 40°C for 4 h, centrifuged, and dried in vacuo. The derivatized oligosaccharide mixtures were redissolved in 400 μl of deionized water for fluorescence detection (FL)-HPLC and LC-MS analyses.

FL-HPLC and LC-MS analyses.

The 2-AB-labeled xyloglucans were analyzed by FL-HPLC and LC-MS. FL-HPLC analysis was performed with a Hitachi Elite LaChrom HPLC system (Hitachi, Tokyo, Japan) connected to a fluorescence detector (L-2485; Hitachi). The samples were analyzed on a Kinetex reverse-phase C18 column (150 by 3 mm, 2.6 μm; Phenomenex, Torrance, CA, USA) at 40°C. The column was first eluted with isocratic 10% (vol/vol) methanol in H2O (with 0.1% TFA) for 10 min at 0.2 ml/min, followed by a linear gradient of 10 to 20% methanol in H2O for 90 min and then a linear gradient of 20 to 100% methanol in H2O for 15 min, and kept at 100% methanol for 30 min. Elution was monitored with a fluorescence detector with an excitation wavelength of 330 nm and an emission wavelength at 420 nm. LC-MS analysis was performed with a Shimadzu Prominence UFLC system (Shimadzu, Kyoto, Japan) coupled to an AB Sciex QTrap 4500 mass spectrometer (AB Sciex, Framingham, MA, USA) with an electrospray ionization source. The LC-MS analysis was performed with the same column and the same HPLC program as aforementioned for FL-HPLC. The mass spectrometer was operated in positive mode, and ions at m/z 200 to 2,000 were scanned. Quantification of extracted ions was normalized by 2-AB-derivatized glucose.

Characterization of bacterial organic acid production.

End products of bacterial fermentation were quantitated by HPLC. Bacterial strains were initially propagated as described above. Cell-free supernatants from microcentrifuge tubes were obtained at early stationary phase, filtered through a 0.22-μm filter following centrifugation, and stored at −80°C until analysis. Organic acids were quantified with an Agilent 1260 Infinity HPLC system (Agilent Technologies, Santa Clara, CA) equipped with a Wyatt Optilab T-rEX refractive-index detector (Wyatt Technology Corp., Santa Barbara, CA). Separation was carried out with an Aminex HPX-87H column (7.8 mm [inside diameter] by 300 mm; Bio-Rad Laboratories, Hercules, CA) at 50°C in a mobile phase of 5 mM H2SO4 at a flow rate of 0.6 ml/min with a 50-μl injection volume. Organic acids (i.e., acetic acid, lactic acid, and formic acid) were acquired from Sigma-Aldrich Co. (St. Louis, MO). Metabolite concentrations were calculated from standard curves derived from external standards for six different concentrations (0.05, 0.1, 0.5, 1, 5, and 10 mg/ml) and converted to millimolar values. Metabolite profiling was carried out in triplicate, and each measurement was performed in duplicate.

Gene expression‎ by qRT-PCR.

Relative gene expression‎ was performed by quantitative real-time PCR (qRT-PCR). Four-milliliter samples were collected at mid-exponential phase, pelleted at 12,000 × g for 2 min, and stored in 1 ml of Ambion RNAlater (Life Technologies, Carlsbad, CA). Total RNA was extracted with the Ambion RNAqueous minikit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer's instructions. The cells were placed in lysis buffer, transferred to MP Bio Matrix E tubes, and subjected to bead beating with a FastPrep 24 instrument (MP Biomedicals, Santa Ana, CA) (2 × 5.5 m/s for 30 s). Total RNA was eluted in 50 μl of EB solution and immediately subjected to DNase treatment with the Ambion Turbo DNA-free kit (Life Technologies, Carlsbad, CA) with 1 μl of DNase I for 0.5 h. Total RNA was converted to cDNA with the High Capacity cDNA reverse transcription kit (Applied Biosystems, Carlsbad, CA) in accordance with the manufacturer's instructions. cDNA concentrations were measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Agawam, MA). qRT-PCR analysis was performed with a 7500 Fast real-time PCR system (Applied Biosystems, Singapore) with PowerUP SYBR green master mix (Applied Biosystems, Foster City, CA) and the parameters suggested by the manufacturer. Primers were designed with Primer3 (Table 5; http://frodo.wi.mit.edu). The Blon_0393 gene was used as an endogenous control as previously validated (59). Gene expression‎ levels during growth on glucose (2%, wt/vol) were used as a reference. Results were expressed as fold changes relative to the control. Bacterial growth was performed in triplicate with triplicate measurements by qRT-PCR.

TABLE 5.

Primers used in this study

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Statistical analysis.

Bacterial growth kinetics were subjected to two-way analysis of variance (ANOVA) and Tukey's honestly significant difference test for multiple comparisons of strains within a treatment compared with the positive control. Metabolite concentrations were subjected to two-way ANOVA, and Sidak's correction was used to account for multiple comparisons. Significant differences in modeled syntrophic interactions were determined by paired t test for bacterial growth kinetics. The fold change in gene expression‎ compared to the control was analyzed by paired t test.

Supplementary Material

Supplemental material

supp_83_17_e01097-17__index.html (2.4KB, html)

ACKNOWLEDGMENTS

We thank Micha Peleg and Mark D. Normand for helpful discussions on microbial growth curve kinetics and Asha Rani for critical review of the manuscript. We thank the Viticulture and Enology Culture Collection for providing B. longum UCD401.

Ocean Spray Cranberries, Inc., and the University of Massachusetts Graduate School are acknowledged for funding. In addition, the University of Massachusetts Innovation Institute is acknowledged for facilitating industrial sponsorship. Instruments used for chemical analysis of oligosaccharides were supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health (2 P20 GM103430). NMR spectroscopy data were acquired at a research facility supported in part by National Science Foundation EPSCoR Cooperative Agreement EPS-1004057. The sponsors did not have any role in study design, data collection and interpretation, or the decision to submit the manuscript for publication.

Footnotes

Supplemental material for this article may be found at https://doi.org/10.1128/AEM.01097-17.

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