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Valorization of pineapple processing residues through acetification to produce specialty vinegars enriched with red-Jambo extract of Syzygium malaccense leaf

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• Published: 12 November 2022

Valorization of pineapple processing residues through acetification to produce specialty vinegars enriched with red-Jambo extract of Syzygium malaccense leaf

• Fernanda Aparecida Brocco Bertan, 
• Eduardo da Silva Pereira Ronning, 
• Marcelo Luis Kuhn Marchioro, 
• Tatiane Luiza Cadorin Oldoni, 
• Robert F. H. Dekker & 
• Mário Antônio Alves da Cunha 

Scientific Reports volume 12, Article number: 19384 (2022) Cite this article

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Abstract

The present study proposes the production of vinegars from pineapple processing residues as an eco-friendly strategy for adding value and economic strengthening of the production chain. Pineapple pulp and peel wines were produced and acetificated to vinegar by wild strains of acetic bacteria using Orlean’s method (traditional system) followed by enrichment with leaf extract of Red-Jambo, Syzygium malaccense. Appreciable phenolic contents and antioxidant potential were found in pulp and peel vinegars with the added leaf extract. Catechin, epicatechin and caffeic, p-coumaric, ferulic, and gallic acids were the main phenolic compounds found in peel vinegar. The enrichment of the vinegar with the extract promoted an increase in the content of polyphenols (443.6–337.3 mg GAE/L) and antioxidant activity. Peel wines presented higher luminosity (L*) and higher saturation index (C*), and their color tended more toward yellow than pulp wines. Acetification reduced the saturation index (C*) and led to the intensification of the hue angle in the peels vinegar. Each type of pineapple vinegar produced showed biocidal activity against different bacteria and yeast, and the addition of leaf extract potentiated the antimicrobial activity of peel vinegar, especially against Staphalococcus aureus. The vinegars developed could find an attractive market niche in the food sector.

초록

본 연구는 파인애플 가공 잔여물을 활용한 식초 생산을

친환경적 전략으로 제안하여

생산 가치 향상과 경제 강화에 기여하는 것을 목표로 합니다.

파인애플 펄프와 껍질로 와인을 제조한 후,

Orlean의 전통적 방법(Orlean’s method)을 통해

야생 아세트산균으로 식초로 발효시켰으며,

이후 Syzygium malaccense(Red-Jambo) 잎 추출물로 강화했습니다.

잎 추출물을 첨가한 펄프와 껍질 식초에서

유의미한 페놀 성분과 항산화 잠재력이 확인되었습니다.

껍질 식초에서 주요 페놀 성분으로

카테킨, 에피카테킨, 카페인산, p-쿠마릭산, 페룰릭산, 갈릭산이 검출되었습니다.

추출물로 식초를 강화하면 폴

리페놀 함량(443.6–337.3 mg GAE/L)과 항산화 활성이 증가했습니다.

껍질 와인은 펄 와인보다 높은 밝기(L*)와 포화도 지수(C*)를 보였으며,

색상은 펄 와인보다 노란색으로 더 기울어졌습니다.

아세트화 과정은

껍질 식초의 채도 지수(C*)를 감소시키고 색상 각도를 강화시켰습니다.

각 유형의 파인애플 식초는

다양한 세균과 효모에 대한 살균 활성을 나타냈으며,

잎 추출물의 추가 투여는 특히 Staphalococcus aureus에 대해 껍질 식초의 항균 활성을 강화시켰습니다.

개발된 식초들은

식품 산업에서 매력적인 시장 니치를 찾을 수 있을 것입니다.

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Introduction

Vinegar is an ancient and versatile product whose production has been known for at least 5000 years. Its origin is probably associated with the production of the first wines, which gave rise to the product by the natural oxidation of ethanol to acetic acid1. Vinegar is a condiment used not only as a seasoning but in preserving the color, odor, and quality of processed foods, such as canned vegetables, ketchup, mustard, and sauces among others. In addition to the food sector, vinegar is also used to clean environments, neutralize odors, eliminate mites, and remove encrustations from domestic objects, among other purposes2.

The production of vinegar takes place through two traditional biotechnological processes. The first corresponds to the alcoholic fermentation carried out by yeasts of the genus Saccharomyces, which converts the fermentable sugars of the must into ethanol. The second process involves the oxidation of ethanol to acetic acid by acetic acid bactéria. Apparently, species of the genera Acetobacter and Komagataeibacter (many of them relocated from the genus Gluconacetobacter) are dominant in the acetification process, due to their high tolerance to acetic acid and preference for ethanol3,4. Such genera are characterized by the ability to convert ethanol into acetic acid by oxidation, as shown in the scheme described in Fig. 1.

소개

식초는 최소 5,000년 전부터 생산이 알려진 고대적이고 다용도적인 제품입니다. 그 기원은 아마도 첫 번째 와인의 생산과 관련이 있으며, 에탄올의 자연적 산화 과정을 통해 아세트산으로 변환되어 이 제품이 탄생했습니다1. 식초는 조미료로 사용될 뿐만 아니라 가공 식품의 색상, 향기, 품질을 보존하는 데도 사용됩니다. 예를 들어 통조림 채소, 케첩, 머스타드, 소스 등이 있습니다. 식품 분야 외에도 식초는 환경 청소, 냄새 중화, 진드기 제거, 가정용품의 침전물 제거 등 다양한 목적으로 사용됩니다2.

식초의 생산은

두 가지 전통적인 생물공학 과정을 통해 이루어집니다.

첫 번째 과정은

Saccharomyces 속의 효모에 의해 수행되는

알코올 발효로, 포도주액의 발효 가능한 당분을 에탄올로 전환합니다.

두 번째 과정은

아세트산 박테리아에 의해 에탄올이

아세트산으로 산화되는 과정입니다.

Acetobacter 및 Komagataeibacter 속의 종(그 중 다수는 Gluconacetobacter 속으로 재분류됨)은

아세트산에 대한 높은 내성과 에탄올에 대한 선호도로 인해

아세트산화 과정에서 우세합니다3,4.

이러한 속은

에탄올을 산화하여 아세트산으로 전환하는 능력을 특징으로 하며,

이는 그림 1에 설명된 도식에서 보여집니다.

Figure 1

Steps involved in the bioprocess of vinegar production in submerged systems (Figure Created with BioRender.com).

Full size image

Vinegar can be obtained from different raw materials, such as fruits, cereals, honey, and ethanol, and the product can also be produced from a mixture of these. Solid state fermentation is traditionally used in Asia for commercial vinegar production. Cereals such as sorghum and rice are used as raw materials in obtaining traditional vinegar in China, such as Shanxi-aged vinegar, Zhenjiang-aromatic vinegar and aged-vinegar from Tianjin Duliu1,5. In the fermentation of Chinese cereal vinegars, substrate degradation and fermentation are allowed to occur simultaneously, with the microbiota changing continuously throughout the process, which comprises saccharification, alcoholic fermentation and acetification1,6. Submerged fermentation is most widespread in Europe7,8 and other western countries, which allows vinegar production on a larger scale.

The most consumed vinegar in Brazil is that obtained from ethanol, wine, and apple cider by submerged fermentation. However, fruit vinegar recently has attracted the attention of consumers due to the functional benefits arising from its antioxidant, antimicrobial, antiallergic, and hepatoprotective activity. Fruit vinegars possess several nutrients, including amino acids, sugars, vitamins, and minerals, which provide energy, regulate cellular metabolism, and immunomodulation, among others. In addition, fruit vinegar products contain bioactive components such as organic recognized antioxidant activity; they also regulate lipid metabolism and blood pressure, control glucose levels, and offer liver protection, in addition to having anti-fatigue and antitumor properties1. Acids, polyphenols, melanoidins, and tetramethylpyrazine with.

Another relevant aspect of fruit vinegar production is the possibility of using surplus fruit as well as fruit residues, such as peels and stalks9. Among fruits are pineapples (Ananas comosus [L.] Merr.) that belong to the Bromeliaceae family, and are a good example considering their worldwide production and consumption. The consumption of pineapples in the minimally-processed form, either as juice or sliced and canned has grown in recent years. Consequently, there is an increase in the generation of processing residues that are basically constituted of peels and pomace. Such residues still have a certain sugar content and are rich in bioactive compounds, and therefore, could be used to obtain new value-added products, including specialty vinegars10.

Pineapple peels make up 37% of the fruit, and only a tiny amount of this biomass residue is used commercially, e.g., as fertilizer, or as an animal feed. Pineapple peel waste has also been used as a raw material in extracting pectin, as a fermentation substrate for the production of solvents (ethanol, butanol), and biogases (hydrogen, methane), as well as sources of bromelain (protease enzymes), phenolic flavor compounds, antioxidants, and organic acids11. Peels offer a possible source for extracting bioactive compounds such as polyphenols, and antioxidants for use in intelligent packaging12. Moreover, pineapple peel extracts have recently been used as a low-cost electrolyte in the remediation process of chromium-contaminated soils13, and has also demonstrated anti-malarial, anti-nociceptive, and anti-inflammatory activities14.

식초는

과일, 곡물, 꿀, 에탄올 등 다양한 원료에서 얻을 수 있으며,

이러한 원료의 혼합물로부터도 생산될 수 있습니다.

아시아에서는

상업용 식초 생산에 전통적으로 고체 발효 방법이 사용됩니다.

중국에서는 수수, 쌀 등 곡물을 원료로 전통 식초를 생산하며,

대표적인 예로 산시(Shanxi) 숙성 식초, 진장(Zhenjiang) 향식 식초, 천진 두류(Tianjin Duliu) 숙성 식초 등이 있습니다.

중국 곡물 식초의 발효 과정에서는

기질 분해와 발효가 동시에 진행되며,

미생물 군집이 지속적으로 변화하는 과정을 포함합니다.

이 과정은

당화, 알코올 발효, 아세트산화 단계로 구성됩니다.

침지 발효는

유럽7,8 및 기타 서구 국가에서 가장 널리 사용되며, 대규모 식초 생산이 가능합니다.

브라질에서 가장 많이 소비되는 식초는

에탄올, 와인, 사과 주스를 침지 발효로 생산한 것입니다.

그러나

최근 과일 식초는

항산화, 항균, 항알레르기, 간 보호 활동에서 비롯된

기능적 이점으로 인해 소비자의 관심을 끌고 있습니다.

과일 식초는

아미노산, 당분, 비타민, 미네랄 등 다양한 영양소를 함유해

에너지 공급, 세포 대사 조절, 면역 조절 등에 기여합니다.

또한 과일 식초 제품은

유기농 항산화 활성을 인정받은 생물활성 성분을 포함하며,

지질 대사 조절, 혈압 조절, 혈당 조절, 간 보호 효과, 피로 회복 및 항종양 성질을 갖추고 있습니다1.

산, 폴리페놀, 멜라노이드, 테트라메틸피라진 등이 포함되어 있습니다.

과일 식초 생산의 또 다른 중요한 측면은 과일 잔여물(껍질, 줄기 등)을 활용할 수 있다는 점입니다.9

과일 중에서는 브로멜리아과에 속하는 파인애플(Ananas comosus [L.] Merr.)이

전 세계적인 생산량과 소비량을 고려할 때 좋은 예시입니다.

최소 가공 형태로 소비되는 파인애플(주스나 슬라이스 및 통조림 형태)의 소비량이 최근 몇 년간 증가했습니다.

이에 따라 가공 잔여물(주로 껍질과 펄프)의 발생량이 증가했습니다.

이러한 잔여물은 여전히 일정량의 당분을 함유하고

생물활성 화합물이 풍부해, 특수 식초 등 새로운 부가가치 제품 생산에 활용될 수 있습니다10.

파인애플 껍질은 과일의 37%를 차지하지만,

이 생물질 잔여물의 상업적 활용은 극히 제한적이며,

예를 들어 비료나 동물 사료로 사용됩니다.

파인애플 껍질 폐기물은

펙틴 추출의 원료, 용매(에탄올, 부탄올) 및 바이오가스(수소, 메탄) 생산을 위한 발효 기질,

브로멜라인(단백질 분해 효소),

페놀성 향료 화합물, 항산화제, 유기산 등의 원천으로 활용되었습니다11.

껍질은

폴리페놀과 항산화제와 같은 생체활성 화합물을 추출하는 잠재적 원료로,

지능형 포장재에 활용될 수 있습니다12.

또한 파인애플 껍질 추출물은 크롬 오염 토양의 정화 과정에서

저비용 전해질로 최근 활용되었으며13,

말라리아 억제, 통증 완화, 항염증 활성을 보여주었습니다14.

A strategy still little explored by the vinegar industry is the enrichment of the content of functional compounds in vinegars, which can easily be achieved by adding plant extracts rich in bioactive compounds. In this context, the red-Jambo extract of Syzygium malaccense (L.) Merr. & L.M. Perry) leaves, is rich in bioactive compounds and could be used as an additive to enhance the quality of unique vinegars. Red-jambo leaves are rich in flavonoids such as catechin, mearnsitrin, myricitrin, quercitin, and the anthocyanins: cyanidin-3,5-O-glucoside, cyanidin-3-O-glycoside and peonidin-3-O-glucoside15.

S. malaccensis (L.) Merr. & Perry belongs to the Myrtaceae family of Asian origin (India and Malaysia), but is also found in Australia, the Caribbean, and Brazil. The species grows rapidly reaching 12- to 15- meters in height, possessing a pyramidal or cylindrical crown, being used ornamentally for its beauty and being able to bloom 2 to 3 times a year in Brazil and the Caribbean varieties16.

There are almost no scientific reports and information about enriching the quality of fruit vinegars with extracts or tinctures from plants and herbs. Therefore, the objective of the present study was the elaboration and characterization of vinegars made from pineapple pulp and peels to which was added an extractive of red-Jambo leaves.

The focus of this work is on using the solid waste from processing pineapples to obtain value-added products (gourmet vinegars) with perspectives of sustainability, and strengthening of the circular bioeconomy. A new alternative value-added product is proposed for the management and direction of solid processing waste generated at both small and large pineapple processing companies, and companies producing fruit juices or those selling the fruit in the minimally-processed form. Adding value to this processing waste stream means strengthening the three basic pillars of sustainable development, which include attention to the economy, society and the environment.

We herein report on the vinegars produced from pineapple pulp and peel with added leaf-extract of S. malaccense (L.) which are rich in phenolic acids and flavonoids and possess antimicrobial activity. The vinegars obtained could potentially contribute to nutritional and health benefits for the consumer.

식초 산업에서 아직 충분히 탐구되지 않은 전략은

식초의 기능성 화합물 함량을 풍부하게 하는 것으로,

생물활성 화합물이 풍부한 식물 추출물을 추가함으로써 쉽게 달성할 수 있습니다.

이 맥락에서 Syzygium malaccense (L.) Merr. & L.M. Perry) 잎의 레드-자모 추출물은

생물활성 화합물이 풍부하며 독특한 식초의 품질을 향상시키기 위한 첨가제로 사용될 수 있습니다.

붉은 자모 잎은

카테킨, 메르니스틴, 미리시트린, 케르시틴과 같은

플라보노이드 및 안토시아닌인 시아니딘-3,5-O-글루코사이드, 시아니딘-3-O-글루코사이드, 페오니딘-3-O-글루코사이드를

풍부하게 함유하고 있습니다15.

S. malaccensis (L.) Merr. & Perry는

아시아 원산지(인도 및 말레이시아)의 Myrtaceae科에 속하지만,

호주, 카리브해, 브라질에서도 발견됩니다.

이 종은 빠르게 성장하여 12~15미터의 높이에 달하며,

피라미드형 또는 원통형의crown을 가지고 있으며,

아름다움으로 인해 장식용으로 사용되며,

브라질과 카리브해 품종에서는 연간 2~3회 꽃을 피웁니다16.

식물 및 허브의 추출물이나 팅크를 사용하여 과일 식초의 품질을 향상시키는 것에 대한 과학적 보고나 정보는 거의 없습니다.

따라서 본 연구의 목적은 파인애플 펄프와 껍질로 만든 식초에 빨간 자모 잎 추출물을 추가하여 제조 및 특성을 분석하는 것입니다.

이 연구의 초점은 파인애플 가공 과정에서 발생하는 고체 폐기물을 활용해 지속 가능성을 고려한 고급 식초(가르메 식초)와 같은 부가가치 제품을 생산하는 데 있습니다.

이는 소규모 및 대규모 파인애플 가공업체, 과일 주스 제조업체, 또는 최소 가공 형태로 과일을 판매하는 업체에서 발생하는 고체 가공 폐기물의 관리 및 활용 방안을 제시하는 새로운 대안적 부가가치 제품을 제안합니다. 이 가공 폐기물 흐름에 가치를 추가하는 것은 경제, 사회, 환경의 세 가지 기본 기둥을 강화하는 것을 의미합니다.

본 연구에서는 S. malaccense (L.) 잎 추출물을 추가한 파인애플 펄프와 껍질로 제조된 식초를 보고합니다. 이 식초는 페놀산과 플라보노이드가 풍부하며 항균 활성을 가지고 있습니다. 얻어진 식초는 소비자의 영양 및 건강에 기여할 잠재력을 가지고 있습니다.

Material and methods

Microorganisms, materials, and red-Jambo leaf extract

Saccharomyces cerevisiae f. r. bayanus (Fermol Perlage) used in this study was provided by the company AEB Biochemistry Latin American AS (Brazil). The wild culture of acetic acid bacteria used in the acetification of wines was isolated from colonial red grape (Vitis labrusca) vinegar produced in the southwestern region of Paraná, Brazil17. Analytical reagents and culture media were purchased from Merck S/A (Brazil) with adequate purity grades. Chromatographic standards (HPLC grade, with purity ≥ 99%) of phenolic acids and flavonoids were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Pineapple peels were obtained in the laboratory from healthy and ripe fruits (Smooth Cayenne variety) acquired in the municipality of Pato Branco (Paraná, Brazil). The fruits were sanitized in sodium hypochlorite solution (100 ppm, 15 min), rinsed in running water, and the peels were then separated from the pulp using stainless steel knives.

Red-Jambo (Syzygium malaccense) leaves were dehydrated at 35 °C, and the extract was obtained by subjecting the leaves to hydroalcoholic extraction (40% ethanol: 60% water) at 80 °C for 45 min as previously optimized by Savi et al.18. The extract was concentrated in a rotary evaporator to eliminate ethanol and lyophilized it for later use.

Inoculum preparation for alcoholic and acetic fermentation

The commercial yeast Saccharomyces cerevisiae r.f. bayanus was rehydrated and cultivated in malt-extract broth (20 g/L malt extract, 1 g/L peptone, and 20 g/L glucose) in 250-mL Erlenmeyer flasks (100 mL of culture medium) in an orbital incubator (shaker) at 28 °C, 150 rpm for 24 h. The cultured cells were recovered by centrifugation (1500 × g, 15 min), washed with sterile isotonic saline solution, transferred to flasks containing 90 mL of freshly prepared malt-extract broth, and cultured for 12 h (28 °C, 150 rpm). The pre-inoculum yeast cells were recovered by centrifugation (1500 × g, 15 min), washed, and resuspended in 300 mL of pineapple juice to obtain a concentration of 1 × 106 cells/mL, which was used as the inoculum in the alcoholic fermentation step17.

Acetic acid bacteria were isolated from unpasteurized vinegar (strong vinegar) acquired from a small rural property in Pato Branco, Paraná. A volume of 10 mL of strong vinegar was transferred to 100 mL Erlenmeyer flasks containing 90 mL of G-Y medium (100 g/L glucose, 10 g/L yeast extract) supplemented with 100 mg/L of natamycin to inhibit the growth of yeasts and fungi. The bacterial culture was kept in an orbital incubator (shaker) for 24 h under agitation (150 rpm), and at 28 °C. The cultured cells were next separated from the medium by centrifugation (1500 × g, 15 min), washed with sterile saline solution (0.9 g NaCl/100 mL), resuspended in 20 mL of distilled water, and then used as the inoculum in the acetification of pineapple pulp and peel wines17.

Obtaining the must and fermentation

Pineapple fruit peels were cut into strips and processed in a blender using a ratio of peels to drinking water of 1:3 (w/v). The fruit pulp was cut into cubes and crushed in a blender at maximum speed until complete liquefaction. The pH of the broths obtained from the peel or pulp was adjusted to 4.0 with 1 mol/L NaOH solution and submitted to enzymatic hydrolysis using the enzyme complex Pectinex Ultra SP-L® (3800 PGNU/mL; Novozymes, Bagsvaerd, Denmark) in the proportion of 15 mL per 100 g of peel or pulp, and incubated at 30 °C for 70 min under agitation (150 rpm).

After hydrolysis, both broths were heated at 90 °C for 5 min to terminate enzyme activity, and the hydrolyzate cooled in an ice-bath. The total soluble solids content of the broths was corrected (chaptalization) with sucrose to 18°Brix. The chaptalized broths were supplemented with Enovit (30 g/hL, a commercial yeast growth activator) and pasteurized at 65 °C for 30 min to obtain chaptalized musts used in alcoholic fermentation.

The alcoholic fermentations were carried out in a fermenting bucket with a coupled S-shaped airlock under static conditions at 28 °C. A volume of 2.7 L of must and 300 mL of inoculum (2 × 106 yeast cells/mL) was used in each fermentation run, and samples removed at 24-h intervals for analyzes (see below).

The wines produced were separated from the lees, peel, and pulp by centrifugation (1500 × g, 20 min), supplemented with the mineral complex Acetozyn® (1.5 g/L. Heinrich Frings GmbH & AMP; Co, USA) and transferred into a 2.5-L Grapia barrel for the acetic fermentation step. The total working volume was 1500 mL, and the inoculum volume was 150 mL, as previously described by Fonseca et al.17. The barrels used for acetification were kept at 25 °C during the acetic oxidation step. The process was accompanied by determining the pH, total acidity expressed as acetic acid (%), and acetic acid content (g/L).

The vinegars produced were subjected to centrifugation under refrigeration (1500 × g, 30 min at 4 °C) for clarification and then enriched with the lyophilized red-Jambo leaf extract of Syzygium malaccense (500 mg of extract/L). The final product was placed in 250 mL glass bottles and then subjected to slow pasteurization in a water bath at 62 °C for 30 min19.

Analytical determinations

The lyophilized red-Jambo leaf extract was characterized in terms of total phenolic content by the Folin–Ciocalteau spectrophotometric method20, and antioxidant potential by the radical scavenging methods: ABTS21, DPPH22 and ferric reducing antioxidant power (FRAP)23.

The alcoholic fermentation runs were sampled every 24 h, and determinations were conducted for total soluble solids (manual refractometer), pH (pH meter), titratable acidity (235/IV method)24 , and total reducing sugar content by the method DNS19, ethanol concentration (HPLC), and yeast cell counts were performed on plating the fermentation broth on Sabouraud-agar plates. The wines obtained were also characterized in terms of titratable acidity (235/IV method)24, and total phenolic content (Folin–Ciocalteau method), bioactive compounds (HPLC), and antioxidant activity (ABTS, DPPH and FRAP methods).

The vinegar samples were analyzed for pH (pH meter), total acidity (504/IV method), mineral residues (incineration at 550 °C), reduced dry extract (509/IV method), density (specific weight at 20 °C)24, total sulfur dioxide content by the Ripper method25 levels of acetic acid and ethanol (HPLC), total reducing sugars19 total phenolics20, bioactive compounds (HPLC), antioxidant activity (ABTS, DPPH and FRAP methods), color by the CIELab system (L * a * b* color space).

Antimicrobial activity was eval‎uated by the disc-diffusion method and broth microdilution to determine the minimal inhibitory concentration (MIC) against microbial strains of clinical importance18.

Phenolic acids and flavonoids were analyzed by HPLC–DAD using a 920 LC chromatographic system (Varian Inc., Walnut Creek, CA, USA), equipped with a C18 column. The column was kept at 30 °C during the analysis, and the injection volume of the samples (wine, vinegar, or properly diluted red-Jambo extract) was 10 μL. The mobile phase consisted of a gradient mixture of 2% (v/v) aqueous acetic acid solution (Solvent A), and 40% (v/v) acetonitrile acidified with 2% aqueous acetic acid solution (Solvent B) with a flow rate of 1 mL/min. The gradient commenced with 5% solvent B adjusted to 20% and run for 2 min, 25% B for 15 min; 85% B for 25 min and then held for 5 min; 20% B at 33 min; 5% B for 16 min with an 8 min conditioning step. Peak areas were determined at 280 nm for gallic acid, vanillic acid, and flavonols: catechin and epicatechin; 300 nm for coumaric acid and salicylic acid; 320 nm for caffeic and ferulic acids and 360 nm for flavonoids: rutin and quercetin.

The contents of ethanol, acetic acid, and other organic acids present in the wine and vinegar samples were determined by HPLC (920 LC chromatographic system equipped with a refractive index detector) and a column HPX-87-H (Bio-Rad, Hercules, CA) maintained at 45 °C, using sulfuric acid solution (0.005 mol/L) as the eluent, at a flow rate of 0.4 mL/min and injection volume of 20 µL per sample. The samples appropriately diluted were first passed through a 45 µm CHOMAFIX filter, and in SEP PACK C18 cartridges (Waters Corporation). Compounds were identified by comparing retention times with authentic standard samples, and quantified by integrating the areas of the respective peaks.

Color analysis

For color eval‎uation, the samples were filtered through 0.45 µm membrane filters, and the color was analyzed in a CR-410 digital colorimeter (Konica Minolta, Japan) using the CIELAB color space. The parameters: luminosity (L*), the chromaticity coordinates a* and b*, and the cylindrical coordinates: hº (hue angle, tonality), and C* (chroma) were measured. After filtering, the samples were placed in the instrument's cuvette, and the measurements were taken in sequence. The color difference between the samples was determined from the Equation bellow.

ΔE∗=(ΔL∗2+Δa∗2+Δb∗2)1/2

(1)

where ΔE corresponds to the total color difference between the samples; ΔL* is the difference in brightness, Δa* is the difference in red and green (+, redder; −,  greener), and Δb* = the difference in yellow and blue (+, more yellow; −, bluer).

Statistical analysis

The results were presented as mean ± standard deviation, and the Kolmogorov–Smirnov test confirmed the normal distribution of variables. The values ​​of the characterization parameters studied in the wines of pulp and peel were compared by the Student's t-test (p < 0.05) using the GraphPad Prism® 8 program (GraphPad Software, Inc.). The characterization parameters of the vinegars were submitted for analysis of variance, and the means were compared by the Tukey test at a significance level of 5% (p < 0.05). OriginPro 8.5 software (OriginLab Corporation, Northampton, MA, USA) was used to create the profile graphs of the fermentations.

Consent to participate

All authors have their consent to participate.

Results and discussion

Total phenolics and the antioxidant potential of Syzygium malaccense leaf extract

Table 1 presents the total phenolic content and antioxidant potential found in the red-Jambo leaf extract that was added to the vinegars produced in the present work.

Table 1 Total phenolics and antioxidant activity in red-Jambo leaf extract.

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Extracts of red-Jambo leaves are still little used. Recent evidence reported that its leaves are rich in phenolic compounds and flavonoids, in addition to having antioxidant activity18. A high content of total phenolics (385.40 mg Gallic Acid Equivalents—GAE/10 g) was found in the lyophilized red-Jambo leaf extract. However, values ​​somewhat higher (537.70 mg GAE/10 g) were reported by Batista et al.26 in the red-Jambo leaf extract obtained using methanol. Different parameters including genetic aspects of the plant, the time and period/season of harvesting, and agronomic conditions related to the crops of the plant, among others, can contribute to obtaining extracts with varying contents of total phenolics.

The antioxidant activity of polyphenols is attributed to their Redox properties, which allows them to act as reducing agents, hydrogen donors, singlet oxygen scavengers, and in metal-chelation27. In this context, appreciable scavenging potential of DPPH (11.82 µmol Trolox equivalent—TE/10 g) and ABTS (41.83 µmol TE/10 g) radicals were found in the present work. Similarly, the leaf extract showed a ferric ion reduction capacity of 54.15 µmol FeSO4/10 g. The values ​​found in the present study are much higher than those reported previously by Savi et al.18 in a similar extract (ABTS: 0.853 µmol TE/kg, DPPH: 0.666 µmol TE/kg, FRAP: 1.267 µmol TE/kg).

Fermentation profile of pineapple must vinification

The profiles of alcoholic fermentations of musts based on pineapple pulp and peel are shown in Fig. 2a,b. The fermentation time was set at 120 h when the release of CO2 ended, and consumption of at least 95% of the sugar content of the musts had occurred. The inoculum used in alcoholic fermentations exhibited high cellular activity concerning growth, substrate consumption, and fermentative activity.

Figure 2

Alcoholic fermentation of musts from (a) pineapple pulp and (b) pineapple peel showing the kinetic profile of reducing sugars (green filled square box), ethanol production (red filled uptriangle), and cell growth (blue filled circle) in the winemaking process.

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The hydration and cultivation of commercial yeast in malt-extract broth and subsequent suspension in pineapple juice resulted in obtaining a metabolically-active inoculum, which was verified by the absence of the lag phase of yeast growth. In this sense, a linear increase in the number of yeast cells was observed during the first 48 h of cultivation, when the maximum cell density (≅ Log 16 CFU/mL) was obtained both in the musts of pineapple pulp (Fig. 2a), and pineapple peel (Fig. 2b).

Cell growth was accompanied by effective substrate consumption and ethanol production, and especially in the pineapple-pulp cultivated cultures. In 72 h of the pulp fermentation, 94.73% of the substrate was consumed, and produced an ethanol concentration of 56.04 g/L. On the other hand, lower values ​​of substrate assimilation (61.60%) and ethanol production (48.61 g/L) were observed in pineapple peel-based musts (72 h). At the end of the alcoholic fermentations, similar values ​​of consumption of total reducing sugars were verified in musts obtained from the pulp (97.60%) and peel (96.70%). The yeast used in the present work also showed similar substrate assimilation values (YC: 96.50%) in must formulated with blackberry and honey as previously described by Fonseca et al.17.

Regarding ethanol accumulation in the fermented broth, higher amounts were observed in musts formulated with the pulp (66.20 g/L) than with the peel (57.40 g/L). The substrate assimilation profile by the yeast observed in 72 h of fermentation suggests that the hydrolyzed pineapple peels present in the must hindered the assimilation of sugars. Such behavior may be related to the complexity of the chemical composition of the pineapple peels. In fact, in addition to the fermentable sugars (glucose, fructose, sucrose) present in the peels, were high contents of structural polysaccharides (cellulose, hemicellulose, pectin) and lignin28, as well as, phenolic compounds, alkaloids, flavonoids, tannins and saponins, and other secondary compounds, which reportedly can have antimicrobial potential29,30.

The fermentative parameters of yeast cultivation in medium based on pineapple pulp and peel are described in Table 2. Determining such parameters is essential to eval‎uate the efficiency and yield of alcoholic fermentation, allowing a better understanding and comparison of the process.

Table 2 Fermentation parameters of pineapple pulp and peel must vinification.

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As observed with the assimilation of fermentable sugars, the ethanol content accumulated at the end of the fermentation run was 15.30% higher in the broth fermented with pineapple pulp compared to that of the peel. Similarly, Alvarenga et al.31 reported that the addition of pineapple peels in musts formulated with the pulp contributed to a reduction in ethanol production. Roda et al.9, and Chalchisa and Dereje32 also reported lower ethanol production values in wines produced from musts formulated with pineapple peels (47.34 g/L and 47.02 g/L, respectively). It is important to note that although greater ethanol production was obtained with the pulp wines, the ethanol yields (substrate to product conversion factor) were similar in both the pulp (YP/S: 0.28 g/g) and peel (YP/S: 0.27 g/g) wines. The alcoholic fermentation efficiency (η) parameter shows a conversion efficiency of 54.80% (1 g of sugar generated 0.28 g of ethanol) of the assimilated sugars into ethanol in the vinification of pineapple pulp must. Similarly, a conversion efficiency of 52.80% (1 g of sugar generated 0.27 g of ethanol) of the assimilated sugars to ethanol was found in the fermentation of must based on pineapple peels. The overall percentage of substrate consumption (YC) was also similar under both fermentation conditions (97.50% and 96.70%). Likewise, little difference was found concerning the process efficiency parameter values (η: 54.8% and 52.8%). Regarding the overall substrate consumption rate (QS), fermentation with pulp showed values 10.9% higher than those found in fermentation with peel. Corroborating the substrate assimilation profile in the exponential growth phase, the maximum specific rate of yeast growth was slightly higher in fermentation with the pulp must (µmax: 0.37 h−1), suggesting that during this phase, a higher percentage of the substrate was directed to yeast cell growth in fermentation of the pulp compared to the peel (µmax: 0.34 h−1).

The fermentation results show that pineapple peels have potential as a raw material for formulating musts intended for alcoholic fermentation. Although the musts formulated with pure pulp stood out in fermentation, the peels showed good fermentative capacity.

Table 3 describes the physical–chemical and bioactivity parameters of wines made with pineapple pulp and peel.

Table 3 Physicochemical and bioactive parameters of pineapple pulp and peel wines.

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In Table 3 (GAE: gallic acid equivalent, TE: trolox equivalents (Trolox-Equivalent Antioxidant Capacity), ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH: 2,2-Diphenyl-1-picrylhydrazyl, FRAP: ferric reducing antioxidant power, nd: not detected, < DL: values below detection limit, L*: luminosity, a*[(−) green to (+) red] and b* [(−) blue to + yellow] chromaticity coordinates, ho, Hue angle and C*, Chromaticity, ΔE: Total color difference. Different letters on the same line differ statistically at the 95% confidence interval (p < 0.05).

Pulp and peel wines had pH values ​​of 3.94 (pulp) and 3.69 (peel) and titratable acidity expressed in acetic acid of 0.76 g/100 mL (pulp) and 0 0.46 g/100 mL (peel). Final pH values ​​between 3.5 and 4.0 were reported by Cunha et al.33 in blackberry wines and Fonseca et al.17 in mixed blackberry and honey wines produced in a vinification process where the same yeast used in the present study was also used. The acidity of a wine is basically due to the presence of organic acids from the fruit itself, such as malic acid, tartaric acid, citric acid, and the production of acids during fermentation, such as acetic acid34. Both pineapple wines obtained showed prominent acidity, especially the pulp wine, possibly because it contained higher amounts of organic acids derived from the fruit itself. Qi et al.34 obtained pineapple wines with lower acidity (0.229 g/100 mL) than was found in this work. This can be attributed both to the characteristics of the fruit and the metabolic properties of the yeast used in the winemaking process. Low pH values ​​during alcoholic fermentation can prevent the growth of undesirable microbiota; an advantage that enhances the quality of the final products.

The alcohol contents present in the pulp and peel wines were 8.39% (v/v) and 7.28% (v/v), respectively. Such values ​​are higher than those reported by Alvarenga et al.31 in wine from the musts formulated with pineapple pulp (6.8% v/v) and pulp plus peel (100 g/kg) (5.9%, v/v). Lower ethanol values ​​were also reported by Roda et al.9 in wines of pineapple peel (6.0%, v/v). It is important to point out that the ethanol content present in wines destined for acetification is a parameter of importance that must be analyzed and adjusted where necessary.

Very high ethanol content in wines can lead to vinegars with a high acetic acid content, resulting in a very acidic product that does not meet the legislation standard. Associated with excess acidity, vinegars with ethanol contents outside the legislation standard can also be produced. Chalchisa and Dereje32 reported the concentration of ethanol in wine should be less than 7.5% to obtain a good quality vinegar. However, it is important to consider the acetification process used, the acetic acid bacteria used in the ethanol-to-acetic acid bioconversion, as well as ethanol losses incurred by evaporation during the acetification process.

Pineapple pulp and peel wines showed density values ​​of 987 and 969 kg/m3, respectively, that agreed with those reported by Akanni Ahoussi et al.35 in pineapple wine (995.0 kg/m3). Similar values ​​(998.2 kg/m3) were also found by Queiroz et al.36 in alcoholic-fermented pineapple juice. The density of the wine varied according to the amount of sugars and ethanol present in the product.

Brazilian legislation37 establishes a maximum limit to the addition of 300 mg/L of sodium metabisulfite in musts that are destined for commercial alcoholic fermentation. No sulfurous anhydride (SO2) residues were detected in the pineapple wines produced in the present study, which was to be expected as the musts were not sanitized with metabisulfite prior to the fermentation stage. Slow pasteurization was the method that we chose to sanitize the musts before alcoholic fermentation.

As shown in Table 3, appreciable total phenolic content was found in both the pulp wine (188.97 mg GAE/L) and peel wine (110.53 mg GAE/L). Different contents of total phenolics have been reported in the scientific literature. Pino and Queris38, when eval‎uating the content of total phenolic compounds in pineapple wines, found lower values ​​(108.0 mg GAE/L) than those found in the present work. On the other hand, Zhang et al.39 found higher values in pineapple peel wine (675.43 mg GAE/L). The different phenolic contents may be associated with the origin of the fruit, the degree of maturation, and the vinification process used39.

The use of high-performance liquid chromatography with diode array detection allowed the identification (Fig. 3) and quantitation of phenolic acids: caffeic acid, coumaric acid, ferulic acid, and the flavonoid catechin in pineapple pulp and peel wines, as outlined in Table 3.

Figure 3

HPLC–DAD chromatograms for samples of (a) pulp wine, (b) peel wine, (c) pulp vinegar, (d) pulp vinegar + extract, (e) peel vinegar, (f) peel vinegar + extract. Gallic acid (1), catechin (2), caffeic acid (3), epicatechin (4), coumaric acid (5), ferulic acid (6).

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Among the phenolic compounds, the flavonoid catechin, was found in higher concentrations in both the pulp (31.63 mg/L) and peel (27.88 mg/L) wines. p-Coumaric acid was also found in relatively considerable amounts (0.35 mg/L and 4.05 mg/L) in both wines. On the other hand, caffeic acid (1.73 mg/L) and ferulic acid (1.48 mg/L) were identified only in the peel wine samples. Catechin (107.39 µmol/L) and ferulic acid (139.70 µmol/L) were the main compounds found in pineapple peel extracts by Li et al.40. Ferulic acid has been reported in pineapple wine by Roda et al.9 at concentrations lower (0.138 µg/L) than those obtained in the present work (1.48 mg/L), and no detectable levels were found in the pulp wine.

The pulp and peel wines, in addition to being rich in total phenolic content, showed high antioxidant potential estimated by the DPPH, ABTS, and FRAP techniques. The pulp wine had an ABTS scavenging capacity of 274.0 µmol TE/100 mL, a slightly higher value than the peel wine (211.0 µmol TE/100 mL). Similarly, appreciable DPPH scavenging potential was also observed in both pineapple wines (129.0 µmol TE/100 mL and 139 µmol TE/100 mL, respectively). Regarding the ferric ion reducing potential, pulp wine (562.6 µmol FeSO4/100 mL) stood out from peel wines (258.1 µmol TE/100 mL). The higher antioxidant capacity found in the pineapple pulp wines can be explained by the higher concentration of phenolic compounds and organic acids commonly found in the fruit pulp, which have antioxidant activity41,42. There is a lack of reports regarding the antioxidant activity of pineapple wines in the scientific literature. Higher values ​​were reported by Fonseca et al.17 in blueberry wine and honey for all of the antioxidant methods eval‎uated. Similarly, Cunha et al.33 also found higher values ​​in blackberry wine for the DPPH (1395.2 µmol TE/100 mL) and ABTS (2124.0 µmol TE/100 mL) methods assessing antioxidant activity.

Organic acids such as ascorbic (vitamin C), citric, malic, oxalic, and succinic acids were found in pineapple pulp and peel used in alcoholic fermentation. Citric (6.20 g/L) and oxalic (7.80 g/L) acids were the predominant organic acids in pulp wine. Oxalic acid (4.91 g/L) and ascorbic acid (1.90 g/L) predominated in pineapple peel wine. Such results show that the pulp and the peels are rich in organic acids.

It is essential to highlight that the composition and the amounts of organic acids present in pineapple fruit can vary greatly depending on the variety, as well as the stage of fruit maturation, considering, for example, that the content of organic acids in the initial stages of fruit development is directly related to the supply of substrates for respiratory processes43.

Regarding the color of the samples, it is important to mention that in the CIELAB space, the luminosity coordinate (L*) varies from black (0) to white (100); the a* coordinate varies between green (-a) and red (+ a), and the b* coordinate varies from blue (-b) to yellow (+ b). The hue angle (hº) starts on the + a* axis (red) and is expressed in degrees: 0° corresponds to + a (red), 90° corresponds to + b (yellow), 180° corresponds to − a (green), and 270° corresponds to − b (blue). C* chroma is 0 at the center of the color axis and increases with distance from it44. Although this method does not provide a precise definition of color, it can effectively show differences in the color of the pulp and peel wine. Instrumental color characterization showed that samples of wine from pulp and peel showed statistically significant differences (p < 0.05) between the color parameters L*, a*, b*, and chroma (C*). The L* values ​​of both pulp and peel wines indicate a tendency towards a grayer color than white. The wine made from peel presented a higher luminosity (52.75) than the wine produced from the fruit’s pulp (42.73). Considering that luminosity is understood as the effectiveness of light in generating the sensation of brightness or clarity when perceived by the human eye, it can be mentioned that the wine sample from pineapple peel tends to be lighter than the pulp sample. In fact, visually, the peel wine sample was visually clearer than the pulp wine.

Values of the a* coordinate (negative values) indicate a green direction, while the b* coordinate values show a yellow trend (positive values) in both samples. The color of the peel wine, in particular, tended more towards yellow (b*: 13.2) than the pulp wine (b*: 2.61), as indicated by the values ​​of the green-yellow color coordinate. Another color aspect that differentiated the peel wine from the wine obtained from the pulp was its saturation (C*: 13.2), which was more noticeable than the pulp wine (C*: 2.24) that presented a more neutral color. Regarding tonality (hº), which is a qualitative attribute of color, there was a significant difference (p < 0.05) between the two samples. Corroborating the results of the parameters L*a*b*, C*, and hº, a statistically significant total difference in color was verified between the pineapple pulp and peel wine samples.

It is worth noting that there was a total difference in color between the pineapple-derived wine samples, with an ΔE of 14.58 being observed. Such behavior could be justified by the fact that both samples presented soft green-reddish tones, but with a tendency to yellow as observed by a* and b* coordinates, in addition to the peel wine presenting saturation values ​​5.5 times higher than the pulp.

Acetic fermentation and pineapple pulp and peel vinegars: physicochemical and bioactive properties

The ethanol-to-acetic acid bioconversion profile of the pineapple pulp (Fig. 4a) and peel (Fig. 4b) wines reveal a good acetification efficiency of the acetic acid bacteria isolated from colonial vinegar and used as inoculum.

Figure 4

Ethanol to acetic acid bioconversion kinetic profile in the acetification of pineapple (a) pulp and (b) peel wines. Ethanol (red filled uptriangle) and acetic acid (blue filled diamond) concentrations.

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The acetification of the pineapple pulp wine occurred in 144 h, when a content of 7.06 g/100 mL of acetic acid, consumption of 88.30% of ethanol, 92.60% efficiency, and 0.49 g/L.h volumetric productivity in acetic acid had occurred. On the other hand, although ethanol assimilation by the acetic acid bacteria was similar in the two acetification processes (88.30% and 86.60%), a longer acetification time was observed in the fermentation of wine formulated with pineapple peels. After 264 h of acetification, the acetic acid content was 5.66 g/100 mL, corresponding to an acetic fermentation efficiency of 87.30% and volumetric productivity of 0.21 g/L h. Tanamool, Chantarangsee and Soemphol45 reported a maximum acetic acid content in vinegar produced from pineapple peels in processes using co-inoculation of yeasts and thermotolerant-acetic acid bacteria of 7.2% (v/v) in 16 days of cultivation.

The higher performance observed in the acetification of the pulp wine compared to the peel wine could be explained in part by the greater nutritional richness of the fruit pulp than the peel. Another aspect that can be considered is the phenolic acid composition of the peel wine. In fact, extracts containing phenolic acids commonly have antimicrobial activity, and such activity can vary considerably depending on the amounts and types of phenolic acids present46. Caffeic acid can interfere with the synthesis of bacterial cell wall macromolecules. Ferulic acid and catechins can modify the charge and hydrophobicity of the cell surface of gram-positive and gram-negative bacteria, leading to cell death by extravasation of the cytoplasmic material. p-Coumaric acid acts as an antimicrobial by disrupting the bacterial cell membrane and binding to bacterial DNA, inhibiting cellular functions46. Table 4 shows the physicochemical parameters of both vinegars.

Table 4 Physicochemical and bioactive parameters of pineapple pulp and peel vinegar.

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In Table 4 (GAE: gallic acid equivalent, TE: trolox equivalents (Trolox-Equivalent Antioxidant Capacity), ABTS: 2,2′-azino-bi(3-ethylbenzothiazoline-6-sulfonic acid), DPPH: 2,2-Diphenyl-1-picrylhydrazyl, FRAP: ferric reducing antioxidant power, nd: not detected, < DL: values below detection limit, L*, luminosity; a*[(−) green to (+) red] and b* [(−) blue to + yellow] chromaticity coordinates, ho, Hue angle and C*, Chromaticity. ΔE: Total color difference. Different letters on the same line differ statistically at the 95% confidence interval (p < 0.05).

The pH ranged from 3.45 to 3.65, which is similar to that reported by Roda et al.9 and Chalchisa and Dereje32 in pineapple peel vinegar; pH values of 3.0 and 3.5, respectively.

Peel vinegar had lower acidity (4.5%) than the pulp vinegar (5.5%), as judged by the lower content of acetic acid present in these vinegars (Fig. 2a,b). Roda et al.9 and Raji et al.47 observed similar acidity values ​​(5.0% and 4.77%, respectively) in the vinegars of pineapple peel.

Brazilian legislation establishes that the minimum volatile acidity of commercially-produced vinegars must be equivalent to 4.0 g of acetic acid in 100 mL of the product. Acidity in acetic acid is a parameter of importance as it reflects the quality of vinegar, since it influences the flavor and acceptability of the product. Vinegar with acidity higher than 5.5% is commonly not acceptable by consumers. On the other hand, vinegar with low acidity produced by the traditional fermentation method is susceptible to contamination by the nematode, Anguillula aceti (vinegar eels)33.

The content of residual ethanol present in pineapple pulp vinegar (0.97%, v/v), pulp vinegar enriched with red-Jambo extract (0.97%, v/v), peel vinegar (0.61%, v/v), and peel vinegar enriched with this extract (0.97%, v/v) are in accordance with Brazilian legislation, which establishes maximum values ​​of 1% ethanol (v/v). The values ​​found were similar to those reported by Roda et al.9 in pineapple peel vinegar (0.50% v/v). It should be noted that small amounts of residual ethanol are necessary since acetic bacteria can promote the degradation of acetic acid in the absence of ethanol48.

Fixed mineral residue (ash) values ​​in fruit vinegar are also established by Brazilian legislation, which must be at least 1 g/L, and in this sense, all commercial vinegar produced presents adequate values. The total dry extract parameter refers to the content of minerals and organic matter that persist after evaporating water and volatile substances from the vinegar33. The values ​​found in pulp vinegar are close to those described in the literature for fruit vinegars. The values ​​of the total dry extract were lower in the vinegar samples obtained from the peels than compared to the pulp vinegar samples. This was probably due to the dilution of the peels in obtaining the must for vinification and wine production. Different contents of total dry extract in fruit peel vinegar have been reported in the literature. Prisacaru et al.49 reported values ​​between 2.11 g/100 mL and 26.43 g/100 mL, while values between 6.90 g/L and 10.59 g/L were mentioned by50. The Brazilian legislation determines a minimum amount of 6 g/L of reduced dry extract in fruit vinegars, the values ​​found in the present work within the current legislation limit.

Regarding density, the vinegar of pulp (1.024 g/mL), peels (1.026 g/mL), pulp with plant extract (1.066 g/mL), and peels with plant extract (1.011 g/mL) are in agreement with what was reported by Raji et al.47 in pineapple peel vinegar (1.08 g/mL). High amounts of phenolic compounds (Pulp Vinegar: 364.45 mg GAE/L, Peel Vinegar: 222.94 mg GAE/L, Pulp + Extract- Vinegar: 443.60 mg GAE/L and Peel + Extract- Vinegar: 337.63 mg GAE/L) were observed in all of the pineapple vinegar samples. It should be noted that pineapple peel vinegar, however, had lower total phenolic contents than those found in the pulp vinegar. In fact, the pulp wine used in acetic fermentation already had a higher content of phenolic substances from the pineapple fruit itself. Another interesting aspect observed was that the wine acetification process increased the phenolic content. This phenomenon occurred because acetic fermentation was conducted by the traditional vinegar system produced in wooden vats. Phenolic compounds migrate from the wooden walls of the acetification barrel and into the vinegar. The compounds supplied by the wood will depend on the type of wood and the roasting of the barrel, the relationship between the contact surface and the volume of liquid, and the contact time51.

The addition of red-Jambo extract in pineapple pulp and peel vinegars promoted the enrichment of the total phenolic content. Among the phenolic substances identified in the samples, the highest epicatechin concentrations were found in the pulp vinegar plus the extract (34.36 mg/L). Caffeic acid (14.89 mg/L) was peel vinegar's most prominent phenolic compound. Gallic (862.61 µg/mL) and caffeic (218.91 µg/mL) acids were reported by Mohamad et al.52 as the major phenolic compounds in pineapple pulp vinegar.

In addition to having high contents of total phenolics, the vinegar produced also had an appreciable ability to scavenge DPPH and ABTS radicals and ferric reducing antioxidant power (FRAP). Regarding the capture capacity of the ABTS radical, values ​​of 410.5 µmol TE/100 mL (Pulp Vinegar), 266.6 µmol TE/100 mL (Peel Vinegar), 547.1 µmol TE/100 mL (Pulp + Extract Vinegar) 337.5 µmol TE /100 mL (Peel + Extract Vinegar) were obtained. The ABTS radical scavenging potential of the pulp vinegar was higher for the peel vinegar. Similarly, pulp vinegar showed greater ferric ion reducing potential (Pulp Vinegar: 402.8 µmol TE/100 mL and Pulp + Extract Vinegar: 675.8 µmol TE/100 mL) compared to peel vinegars (Peel Vinegar: 277.8 µmol TE/100 mL and Peel + Extract Vinegar: 542.3 µmol TE/100 mL).On the other hand, peel vinegar was more efficient in capturing the DPPH radical (peel vinegar: 227.8 µmol TE/100 mL and peel + extract vinegar: 277.5 µmol TE/100 mL) than pulp vinegar (Pulp Vinegar: 216.4 µmol TE/100 mL and Pulp + Extract Vinegar: 249.8 µmol TE/100 mL). The chemical nature of the bioactive compounds present in the vinegars, including chemical structure, polarity and hydrophobicity, strongly influence their free-radical scavenging capacity or reducing antioxidant potential. In this sense, more than one method is commonly used to eval‎uate the antioxidant potential of the same sample since the antioxidant eval‎uation methods are correlated with the mechanisms of antioxidant action53. Several studies of antioxidant activity in vinegar samples have been described in the scientific literature. However, there is some difficulty in comparing results due to the diversity of methods and expression‎ of results. Fonseca et al.17 reported similar values ​​for the scavenging of ABTS (368.39–402.15 μmol TE/100 mL) and DPPH (186.73–211.39 μmol TE/100 mL) radicals in blueberry and honey vinegar. Regarding the FRAP potential, these authors found much higher values ​​(1881.45–1884.5 μmol FeSO4/100 mL) in relation to the vinegar obtained in the present study.

The same organic acids present in the pineapple pulp and peel wines were found in the vinegar samples. Like what was observed in pulp wines, citric (pulp vinegar: 7.41 g/L and pulp + extract vinegar: 7.46 g/L) and oxalic (pulp vinegar: 9.04 g/L and pulp + extract vinegar: 9.09 g/L) acids were the predominant organic acids in pulp vinegar. Oxalic acid (peel vinegar: 4.75 g/L and peel + extract vinegar: 4.78) and citric acid (peel vinegar: 1.46 g/L and peel + extract vinegar: 1.48 g/L) predominated in pineapple peel vinegar. The enrichment of vinegar with red-Jambo leaf extract did not promote statistically significant changes in the composition of organic acids.

Pineapple pulp vinegar showed luminosity (L*: 45.39) close to the spectrum observed in wine (L*: 42.73). On the other hand, the peel vinegar (with and without the addition of red-Jambo leaf extract) showed slightly lower luminosity values ​​(peel vinegar: 42.29 and peel + extract vinegar: 43.12) than those found in the peel wine samples (L*: 52.75). The reduction in luminosity values ​​indicates that the acetic fermentation process carried out in wooden barrels contributes to a certain decrease in the perception of brightness and clarity of the peel vinegar samples. This phenomenon may be associated with the probable extraction of compounds from the wood of the acetification barrel, associated with the chemical composition of these vinegars, which present differences in the concentrations of phenolic compounds compared to pulp vinegar. The color properties of different types of vinegar can change depending upon the color of the raw material and the technology used in the production54.

Regarding the a* coordinate (red index), the acetic fermentation contributed to a green tendency, which was more pronounced in the peel vinegar samples. Similarly, the acetification of wines also reduced the values ​​of the b* coordinate (yellow index: tendency from yellow to blue), especially in the vinegar of peels. The acetification of the wines led to an intensification of the hº coordinate (hue angle), increasing the tendency to green (from yellow to green) in the peel vinegar samples (peel vinegar:135.01 and peel + extract vinegar: 122.64). A more pronounced increase in the values ​​of the hº coordinate was observed in the pulp vinegar samples in relation to the pulp wines, with a tendency to intensify the blue hue (peel vinegar: 206.54, peel + extract vinegar: 205.63). The eval‎uation of the chromaticity index (C*) of the samples indicates that the acetification led to the reduction of the saturation index of the vinegar samples concerning the wines, this phenomenon being more pronounced in the vinegar of pineapple peels. The enrichment of pulp vinegar with red-Jambo leaf extract did not promote statistically significant changes in the color parameters of the pulp vinegar samples (L* a* b*, hº, and C*). On the other hand, adding the leaf extract to the peel vinegar led to changes in the instrumentally-detectable color parameters (L* b*, hº, and C*) (peel vinegar versus peel + extract vinegar).

It is essential to highlight that the eval‎uation of the total color difference (ΔE) indicates that adding the red-Jambo leaf extract to the samples did not promote visually-detectable changes when comparing samples with or without added extract. Some reports in the scientific literature and based on the study described by Stokes et al.55 mention that color differences less than 2.15 are not perceptible to the human eye44. The results of ΔE found in the present work are close to this threshold, especially in relation to vinegar obtained from pineapple peels (Table 4).

Antimicrobial potential

Vinegar has been recognized as an antimicrobial substance for a long time, and several studies have shown vinegars of different origin can act as antimicrobial agents against different pathogens54. Table 5 shows the results of the susceptibility of different bacterial strains (gram-negative and gram-positive) and yeasts to the pulp and peel vinegar produced in the present work. Diffusion disk tests and eval‎uation of the minimum inhibitory concentration (MIC) showed that all vinegars, with or without added red-Jumbo leaf extract in the present study, presented inhibition against the microorganisms studied.

Table 5 Antimicrobial and antifungal potential of pineapple vinegar samples.

Full size table

In Table 5 (AS: standard antimicrobial, Tetracycline for bacteria and Fluconazole for fungi), ID: Diameter of the inhibition zones (inhibition halo), MIC: minimum inhibitory concentration, MBC: minimum bactericidal concentration, MFC: Minimum fungicide concentration, *: no eval‎uated, #: no inhibition, &: commercial alcohol vinegar).

Pineapple pulp vinegar promoted greater inhibition halos against both bacteria and yeasts when compared to peel vinegar. Diffusion disk tests also showed that the enrichment of vinegar with red-Jambo leaf extract potentiated the antimicrobial activity. Another aspect observed is that the pulp and peel vinegars promoted inhibition diameters similar to those observed using commercial alcohol vinegar (4.0 g acetic acid/100 mL).

The minimum concentrations necessary to inhibit the microorganisms studied ranged from 5 µL/mL to 16 µL/mL. This range was wider regarding the bactericidal concentration, ranging from 15.5 µL/mL to 151.5 µL/mL. Similarly, the concentrations required for yeast inhibition ranged from 16 µL/mL to 151.5 µL/mL. These values ​​indicate that the sensitivity of the microorganisms studied against the pineapple vinegar samples was relatively variable. In agreement with the results obtained, Ozturk et al.56 reported a high variability in a study with twenty samples of traditional vinegar produced in Turkey (homemade vinegar) in relation to the sensitivity of the bacteria studied.

Bacillus subtilis (G+) was the microorganism most sensitive to pure pulp vinegar and enriched with the red-Jambo leaf extract, with inhibition at a 5.0 µL/mL concentration and cell death at a concentration of 16.0 µL/mL. Escherichia coli (G−) was the most resistant strain, being inhibited at a concentration of 5.0 µL/mL in pulp vinegar (with or without the added red-Jambo leaf extract) and 16.0 µL/mL in peel vinegar (with or without leaf extract), but showed resistance to the biocidal activity of the vinegar samples. Similarly, Ousaaid et al.57 reported E. coli as the microbial strain most resistant to antimicrobial activity (MIC: 3.125 μL/mL; MBC: 6.25 μL/mL) in apple cider vinegar.

In eval‎uating the antimicrobial capacity against the yeasts, we observed that the pineapple pulp vinegar showed more significant antimicrobial potential against the Candida albicans strain than the peel vinegar. Pulp vinegar was able to inhibit this yeast at a 16.0 µL/mL concentration and promote its death at a 50.0 µL/mL concentration. However, there was no observable antimicrobial potentiation of the red-Jumbo leaf extract against the two Candida tropicalis yeast strains eval‎uated, thus maintaining its minimum inhibitory concentration and fungicidal concentration.

The enrichment of peel vinegar with red-Jambo leaf extract potentiated the antimicrobial activity against the Gram-positive bacterium S. aureus, with MBC values ranging from 50.5 μL/mL (peel vinegar) to 16 μL/mL (peel + extract vinegar). On the other hand, adding the red-Jumbo leaf extract to the vinegar did not potentiate the antimicrobial activity against the other microorganisms. The antimicrobial potential of vinegar is associated with the presence of organic acids, which have antimicrobial activity, especially acetic acid, which can cross the bacterial membrane and promote a reduction in intracellular pH, consequently causing the death of the microorganism57. Weak organic acids cross the cell membrane in the undissociated form and dissociate according to intracellular pH, releasing a proton into the cytoplasm56.

Conclusion

The production of wines and vinegars from pineapple pulp and peels can be a strategic tool for using the entire fruit within a circular economy context. Red-Jambo (Syzygium malaccense) leaf extract studied was rich in phenolic compounds and flavonoids and showed high antioxidant potential in vitro. The yeast strain Saccharomyces cerevisiae r. f. bayanus showed good efficiency in the alcoholic fermentation of pineapple pulp- and peel- based musts. The pulp and peel wines presented appreciable contents of total phenolic compounds, phenolic acids, flavonoids, and organic acids, as well as high antioxidant potential. Higher amounts of phenolic compounds and higher antioxidant capacity were found in pineapple pulp and peel vinegars than in wines indicating that the acetification process contributed to the potentiation of bioactive properties. Adding red-Jambo leaf extract to vinegar contributed to the enrichment of total phenolic compounds and antioxidant activity, and potentiated the antimicrobial activity observed. The pineapple-derived vinegars showed antimicrobial activity with biocidal action against the bacteria Staphylococcus aureus, Bacillus subtilis, Salmonella enterica Typhimurium, and the yeasts Candida tropicalis and Candida albicans. The vinegars produced can be considered specialy vinegars, and their production can contribute to the use of vegetable biomass that is commonly discarded or underused during processing, adding value to the production chain. As a future perspective that could attract consumers and expand the vinegar market, our study highlights using red-Jambo leaf extracts rich in bioactive compounds, including compounds associated with digestive properties.

Compliance

The procedures for collecting and obtaining the studied plant extract followed the relevant institutional, national, and international guidelines and legislation. The voucher specimen was prepared and identified by botanist Dr. Giovana Faneco Pereira, the copy remaining deposited in the Herbarium of the Universidade Tecnológica Federal do Paraná, Campus Pato Branco, under the collection number HPB 1173.

Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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