Production of Indole-3-Acetic Acid in the Plant-Beneficial Strain

<a href="javascript:checkVirus('grpid%3D19iP6%26fldid%3DDqpD%26dataid%3D42%26fileid%3D1%26regdt%3D20160404195921&url=http%3A%2F%2Fcfile300.uf.daum.net%2Fattach%2F2612234D570248EB359D28')"><img src="https://t1.daumcdn.net/daumtop_deco/icon/icon.hanmail.net/editor/p_pdf_s.gif" border="0" alt="첨부파일" class="vam"/> 2006 김영철 IAA-CM06.pdf</a> CURRENT MICROBIOLOGY Vol. 52 (2006), pp. 473–476 DOI: 10.1007/s00284-005-0427-xProduction of Indole-3-Acetic Acid in the Plant-Beneficial Strain Pseudomonas chlororaphis O6 Is Negatively Regulated by the Global Sensor Kinase GacS BeomRyong Kang,1 Kwang Yeol Yang,1 Baik Ho Cho,1 Tae Ho Han,1 In Seon Kim,1 Myung Chul Lee,2 Anne J. Anderson,3 Young Cheol Kim1 1Agricultural Plant Stress Research Center and Biotechnology Research Institute, College of Agriculture and Life Sciences, ChonnamNational University, Gwangju 500-757, Korea 2National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Korea 3Department of Biology, Utah State University, Logan, UT 84322-5305, United States Received: 9 November 2005 / Accepted: 19 January 2006 Abstract. Certain plant growth–promoting bacteria, such as Pseudomonas fluorescens 89B61 and Bacillus p umilus SE34, secreted high levels of indole-3-acetic acid (IAA) in tryptophan-amended medium in stationary phase as determined by chromogenic analysis and high-performance liquid chromatography. Two other growth-promoting strains, P. chlororaphis O6 and Serratia marcescens 90- 166, did not produce these high levels of IAA. However, when the gacS mutant of P. chlororaphis O6 was grown in tryptophan-supplemented medium, IAA was detected in culture filtrates. IAA production by the gacS mutant in P. chlororaphis O6 was repressed in the tryptophan medium by complementation with the wild-type gacS gene. Thus, the global regulatory Gac systemin P. chlororaphis O6 acts as a negative regulator of IAA production fromtrypophan .