스크랩 Ohio State University // Medical School.-임상,논문발표중...2007년

[rejoice]

마이톨의 피부암과 알러지에 대한 치료 효과입니다.

[PC- Ecklonia Cava Extract (= PC ECE //SEANOL)]

7. CANCER
PC- ECE anti-tumor effects currently tested only for dermatologic cancers in mice. No
clinical studies of the anti-cancer potential of PC- ECE have been conducted to date,
although a single murine dermatological cancer study performed on PC- ECE is
summarized below:
Chemoprevention of UVB-induced Skin Carcinogenesis by PC- ECE on SKH-1 Mice
1. Purpose of the Study: Chronic exposure of solar ultraviolet B (UVB) radiation to skin
induces oxidative stress, which plays a crucial role in the induction of non-melanoma
skin cancer. In this study, the research investigated the effect of oral feeding and
topical application of PC- ECE on UVB radiation-induced skin carcinogenesis on
SKH-1 mice.
2. Protocol: SKH-1 hairless mice (N=48) were treated orally or topically with PC- ECE
and irradiated with UVB three times (160mJ/cm2) per week for 26 weeks.
3. Summary of Results - In both dietary and topical treatment of PC- ECE
? Significantly reduced tumor multiplicity by ~50%
? Significantly decreased PGE2 levels by 50~80%
? Significant down-regulation of inflammatory genes (COX2 and iNOS)
was also observed through RT-PCR, Western Blot, etc
? Regulation of other genes are in question
32 - 32 -32
COX-2/iNOS activity COX-2/iNOS Gene Expression‎!
Chemoprevention and Support Program, Division of Hematology and Oncology, Dept. of
Internal Medicine, College of Medicine and Public Health, Ohio State University
The results of this study provide evidence that PC- ECE possesses excellent anti-photo
carcinogenesis effect, which may be associated with the prevention of UVB-mediated
oxidative stress, an inflammation pathway in the skin. The study also indicates potential
for chemoprevention of other types of cancer.8
Another outcome of the study was the finding that PC- ECE affords strong protection
against UVB-induced iNOS suppression and inflammatory damage in the skin. Chronic
exposure to UVB induced substantial increase in iNOS expression‎! together with severe
damage in the skin. However PC- ECE significantly inhibited the production of iNOS by
48-78% depending upon dosage level and delivery method (dietary or topical). 9

8. ALLERGIES
8 H. Hwang, T. Chen, R. Nines, H-C Shin, G. Stoner. Photochemoprevention of UVB ?
induced skin carcinogenesis in SKH-1 mice by brown algae polyphenols. International
Journal of Cancer: 119, 2742-2749 (2006).
9 H. Hwang, T. Chen, G. Stoner, K-B Lee, Y-C Yoo, H-C Shin. Suppression of iNOS
Expression‎! by Phlorotannins in Chronic Exposure of Skin to UVB Radiation. Laboratory
Animal Research 2005; 21(4), 385-389.
33 - 33 -33
PC- ECE has been shown to dramatically relieve allergic reactions without any
drowsiness, dizziness and other side effect of anti-histamine drugs. Its effectiveness has
been demonstrated by allergen-induced murine asthma model by Dr. Chi, Dept. of
Histopathology, University of Washington.
Effect of PC- ECE on Allergen-induced murine asthma model
Airway infiltration and remodeling in chronic asthma is characterized by eosinophils,
mucus cell hyperplasia with mucus hypersecretion accumulation of mononuclear cells in
airway interstitium and sub-epithelial fibrosis of the airway wall. PC- ECE (“KLS” in
test results) was tested in a mouse model of allergen induced chronic lung inflammation
and fibrosis. BALB/c mice, after I.P. OVA sensitization on day 0 and day 14, gave
intranasal (i.n.) inhalation of OVA weekly about day 14-60. The OVA-treated and
challenged mice developed an extensive eosinophil and mononuclear cell inflammatory
response, mucus cell hyperplasia and mucus occlusion of the airway striking feature of
this inflammatory response was the widespread deposition of collagen beneath the airway
epithelial cell layer and also in the lung interstitium in the sites of leukocytic infiltration
that was not observed in the saline-treated control mice.
PC- ECE was tested and found effective in reducing allergic reaction in inflammation.
By feeding at a concentration of 5.4 mg/ml in the drinking water for 12 days, PC- ECE
reduced the airway mucus plugging, and sub-epithelial fibrosis in the OVA-sensitized/
challenged mice. The reduced BAL fluid eosinophil indicated that PC- ECE is effective
in improving the asthmatic lung structures. 12 days of feeding, PC- ECE demonstrated
no pathological alterations in the liver, kidney, spleen, or small intestine.
Summary of results:
- Eosinophil migration in the lung reduced by 75%
- Cellular infiltration (CD4+4 T Cells, resultant cytokines Il-4, 5, 13) reduced by
50%
- Mucus plug in airways reduced by 75%
- Airway epithelial hyperplasia reduced by 75%
- Collagen in lung interstitium (fibrosis, airway remodeling) and smooth muscle
cell thickness reduced by 20% and 32%, respectively