Heat Shock Protein의 조직재생에 관련된 논문들

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열충격 단백질의 효과를 극대화하는

가열식 화침의 효과는 정말 놀랍다. 

panic bird...

Heat Shock Proteins Modulate Keloid Formation

Serhat Totan, MD, Anthony Echo, MD, and Eser Yuksel, MD

Baylor College of Medicine, Michael E. Debakey Department of Surgery, Division of Plastic Surgery,

Houston, TX

Correspondence: aecho@bcm.edu

Published April 29, 2011

참고) keloid

Heat Shock Proteins Modulate Keloid Formation.pdf

Objective: Heat shock proteins (HSPs) modulate the intensity of the inflammatory and synthetic response to stress in wound healing. Induction of HSPs at the site of wounds improves healing by acting as a molecular chaperone. However, the role of HSPs may augment the inflammatory response, leading to an uncontrolled synthetic process. Propensity for keloid development involves genetic predisposition, physical factors, and an aggressive inflammatory response. The aim of this study is to demonstrate the differential expressions of HSPs in keloid and normal tissues. 

- 열충격단백질은 염증의 정도와 상처치유에서 스트레스 반응의 조합을 조절함. 상처위치에서 열충격단백질의 유도는 치유를 촉진함. 하지만 열충격단백질의 역할은 염증반응에서 논란이 있고, 조절되지 못한 합성반응을 야기할 수 있음. 켈로이드 생성의 경향성은 유전적 특성, 물리적요소, 과도한 염증반응때문임. 이 논문의 목적은 켈로이드와 정상조직에서 열충격단백질의 발현을 탐구함. 

Methods: Twenty-five keloid and adjacent normal tissue samples were removed from 24 patients who were between 16 and 45 years of age. Western blot, enzyme-linked immunosorbent assay, and immunofluorescence studies were performed to examine hsp27, hsp47, hsp60, hsp70, and hsp90 levels in keloid and normal tissue. 

- 25개 켈로이드와 주변 정상조직 샘플을 24명 환자(16세에서 45세)로부터 추출.

- western blot등 분자생물학적 검사를 시행함. 

Results: Our results demonstrated a significant overexpression‎ of hsp27, hsp47, and hsp70 in keloid tissue compared to that of normal tissue. Statistical analysis using the Student t test revealed a significant difference between these 2 groups (P < .01), while the expression‎ of hsp60 and hsp90 were not significantly different between the keloid and normal tissue samples. 

- 연구결과 켈로이드 조직에서 열충격단백질 27, 47, 70의 과발현이 나타남. 

Conclusion: The overexpression‎ of HSPs indicates that both a proliferative (hsp70) and a matrix synthesis (hsp47, hsp27) component are present in keloid tissue. From this point of view, it is probable that HSPs play a pivotal role in keloid formation. Unveiling  HSP-keloid interactions may allow us to manipulate the inflammatory and proliferative phases of wound healing with the potential to control keloid formation.

- 열충격단백질의 과발현은 증식(열충격단백질 70)과 기질합성(열충격단백질 47, 27)이 켈로이드 조직에서 나타남. 

- 이러한 관점에서 열충격단백질은 켈로이드 형성에 중요한 역할을 함. 

Heat Shock Protein and Apoptosis in Supraspinatus Tendinopathy

Neal L. Millar MBChB, MRCS (Glasg),

Ai Q. Wei MBBS, Timothy J. Molloy PhD,

Fiona Bonar MBChB, MRCPI, FRCPath(Lond),

George A. C. Murrell MD, DPhil

Abstract 

Heat shock proteins (HSPs) are often upregulated following oxidative and other forms of stress. Based on reports of excessive apoptosis in torn supraspinatus tendon and mechanically loaded tendon cells, we hypothesized heat shock proteins may be present in rodent and human models of tendinopathy due to their central role in caspase dependent apoptotic cell signaling. We used a running rat supraspinatus tendinopathy overuse model with custom microarrays to investigate the process at a genetic level. Additionally torn supraspinatus tendon and matched intact subscapularis tendon samples were collected from patients undergoing arthroscopic shoulder surgery. 

Control samples of subscapularis tendon were collected from 10 patients undergoing arthroscopic stabilization surgery and eval‎uated using semiquantative RT-PCR and immunohistochemistry. We identified substantial upregulation of heat shock proteins and apoptotic genes in the rodent model. We further confirmed increased levels of heat shock protein and apoptotic regulatory genes in human supraspinatus and subscapularis tendon. 

This finding suggests heat shock proteins play a role in the cascade of stress-activated programmed cell death and degeneration in tendinopathy and may provide a novel target in preventing tendinopathies.

Heat Shock Protein and Apoptosis in Supraspinatus Tendinopa.pdf

침과 관련된 열충격단백질 리뷰논문.

A review of the potential effect of EA and moxi on cell rep.pdf

HEAT SHOCK PROTEINS IN DIABETES AND WOUND HEALING

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