2010 12 장미속 유전자원의 유전적 관계와 국내 해당화의 유전적 다양성 15_10-096(한태호)원과지.pdf
Kor. J. Hort. Sci. Technol. 28(6):1003-1013, 2010
장미속 유전자원의 유전적 관계와 국내 해당화의 유전적 다양성
정연화1ㆍ김성태2ㆍ김기준3ㆍ이자현1,4ㆍ기광연5ㆍ한태호1,4*
1전남대학교 식물생명공학과, 2국립원예특작과학원 화훼과, 3아시아종묘 육종연구소,
4전남대학교 농업과학기술연구소, 5전라남도농업기술원 원예연구소
Genetic Relationship of Genus Rosa Germplasm and Genetic Diversity of Rosa rugosa in Korea
Youn-Hwa Joung1, Seung-Tae Kim2, Gi-Jun Kim3, Ja-Hyun Lee1,4, Gwang-Yeon Gi5, and Tae-Ho Han1,4*
1Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, Korea
2Floriculture Research Division, National Institute of Horticultural & Herbal Science, Suwon 440-706, Korea
3Breeding Institution, Asia Seed Co. Ltd., Gwangju 464-863, Korea
4Institution of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea
5Horticultural Research Division, Jeollam-do Agricultural Research & Extension Services, Naju 520-715, Korea
Abstract. Fifty-nine accessions of 23 species in genus Rosa were collected, and 15 accessions of Rosa rugosa
were collected throughout 10 regions of Korea. Their genetic relationship was investigated by using morphological
analysis and RAPD marker. The morphological analysis was measured for 7 quantitative and quantified for
4 qualitative traits. RAPD analysis obtained a total of 959 polymorphic bands by using twenty primers.
Morphological analysis classified most according to the rose section system except for several species. The
cluster analysis of genus Rosa based on RAPD data could identify the subgenus Platyrhodon and Eurosa. The
subgenus Eurosa separated five sections; Gallicanae, Cinnamomeae, Pimpinellifolia, Synstylae, and Caninae.
Correlation analysis between morphological and RAPD analysis showed low significance (r = 0.35). The accessions
of R. rugosa belonged to the section Cinnamomeae clustered into three groups at genetic distance ranging
from 0.28 on the base of RAPD analysis. In conclusion, the genetic relationship of the genus Rosa was consistent
to the previously reported rose section system, and domestic collections of R. rugosa were separated from
3 groups on the base of RAPD marker.
Additional key words: mantel test, morphological analysis, random amplified polymorphic DNA (RAPD),
rose, section, subgenus
*Corresponding author: hanth@chonnam.ac.kr
※ Received 13 May 2010; Accepted 13 October 2010. 본 연구는 농촌진흥청 공동연구사업(과제번호:PJ006816)과 농업유전자원관리기관사업의
지원에 의해 이루어진 것임.
서 언
장미는 국내・외 화훼류 중 선호도와 시장점유율이 높은
절화 작물이다. 국내 재배 면적은 2006년까지 꾸준히 증가
하여 864ha였으나 유류비와 로열티 문제로 인해 2007년 이
후 579ha로 줄어드는 경향을 보인다(MIFAFF, 2008). 특히,
외국 품종에 의존하고 있는 국내 재배 농가의 로열티 지불
액은 2007년 기준으로 약 120억 원으로 추정되고 있어 국내
품종 육성과 보급이 시급하다(Kim 등, 2008). 국내 품종 육
성은 1990년 시작되어 육종의 역사가 짧고 재배 품종간의
단교잡이 이