Systematically exploring the performance of a self-developed.. ('23)

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Systematically exploring the performance of a self-developed Multi-
InDel system in forensic identification, ancestry inference and genetic
structure analysis of Chinese Manchu and Mongolian groups
Qiong Lan a,b, Meiming Cai a, Fanzhang Lei a, Chunmei Shen c,⁎, Bofeng Zhu

a b s t r a c t
The insertion/deletion (InDel) polymorphism has promising applications in forensic DNA analysis. However,
the insufficient forensic efficiencies of the present InDel-based systems restrict their applications in par-
entage testing, due to the lower genetic polymorphism of the biallelic InDel locus and the limited number of
InDel loci in a multiplex amplification system. Here, we introduced an in-house developed system which
contained 41 polymorphic Multi-InDel markers (equivalent to 82 InDels in total), to serve as an efficient and
reliable tool for different forensic applications in the Manchu and Mongolian groups. We demonstrated that
the new system exhibited potential efficiencies for personal identification, parentage testing, two-person
DNA mixture interpretation and ancestry inference of intercontinental populations. Meanwhile, we ex-
plored the genetic backgrounds of the Manchu and Mongolian groups by conducting a series of population
genetic analyses. We showed that the Manchu and Mongolian groups shared closer genetic relationships
with East Asian populations, especially Han Chinese populations in northern China. Moreover, more similar
genetic compositions were detected between the Manchu group and the northern Han populations in this
study, suggesting that the Manchu group had higher genetic affinities with northern Han populations than
the Mongolian group. Overall. this study provided the necessary evidence that these Multi-InDel genetic
markers could play an important role in forensic applications.

https://www.sciencedirect.com/science/article/pii/S0379073823000877

Systematically exploring the performance of a self-developed Multi-InDel system in forensic identification, ancestry inference a

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