exercise-Induced Oxidative Stress: Cellular Mechanisms and Impact on Muscle Force Production

[문형철]

운동과 항노화는 약간의 역설이 있구나.

운동을 하면서 분비되는 유해활성산소, 그리고 그것을 제거하는 항산화제(sod, catalase, glutachione)

요분야에 대해서 이해를 해야겠다. ㅎㅎ

http://physrev.physiology.org/content/88/4/1243.full

Exercise-Induced Oxidative Stress.pdf

Superoxide Dismutase and Catalase in.pdf

panic bird .....

exercise-Induced Oxidative Stress: Cellular Mechanisms and

Impact on Muscle Force Production

Scott K. Powers and Malcolm J. Jackson

Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, Florida; and Division of Metabolic and Cellular Medicine, University of Liverpool, Liverpool, United Kingdom

ABSTRACT

The first suggestion that physical exercise results in free radical-mediated damage to tissues appeared in 1978, and the past three decades have resulted in a large growth of knowledge regarding exercise and oxidative stress. Although the sources of oxidant production during exercise continue to be debated, it is now well established that both resting and contracting skeletal muscles produce reactive oxygen species and reactive nitrogen species. Importantly, intense and prolonged exercise can result in oxidative damage to both proteins and lipids in the contracting myocytes. Furthermore, oxidants can modulate a number of cell signaling pathways and regulate the expression‎ of multiple genes in eukaryotic cells. This oxidant-mediated change in gene expression‎ involves changes at transcriptional, mRNA stability, and signal transduction levels. Furthermore, numerous products associated with oxidant-modulated genes have been identified and include antioxidant enzymes, stress proteins, DNA repair proteins, and mitochondrial electron transport proteins. Interestingly, low and physiological levels of reactive oxygen species are required for normal force production in skeletal muscle, but high levels of reactive oxygen species promote contractile dysfunction resulting in muscle weakness and fatigue. Ongoing research continues to probe the mechanisms by which oxidants influence skeletal muscle contractile properties and to explore interventions capable of protecting muscle from oxidant-mediated dysfunction.

I. INTRODUCTION

Regular physical exercise has many health benefits including a lowered threat of all-cause mortality along with a reduced risk of cardiovascular disease, cancer, and diabetes (45, 77, 283). Paradoxically, it is also clear that contracting skeletal muscles generate free radicals and that prolonged and intense exercise can result in oxidative damage to cellular constituents (10, 86, 98, 107, 165, 321, 325). During the past three decades, our knowledge about the biological implications of exercise-induced oxidative stress has expanded rapidly. Indeed, it is now appreciated that while high levels of free radicals can damage cellular components, low-to-moderate levels of oxidants play multiple regulatory roles in cells such as the control of gene expression‎, regulation of cell signaling pathways, and modulation of skeletal muscle force production (102, 317, 318, 368).

Because of recent advances in the field of exercise and oxidative stress, this is an appropriate time to summarize some of the major principles of exercise-induced oxidative stress and its impact on skeletal muscle function. Our approach will be to provide a critical synopsis of major concepts rather than a detailed analysis of data. We will begin with an overview of oxidant species, antioxidant systems, and the concept of oxidative stress. This will be followed with a historical synopsis of research in the field of exercise-induced oxidative stress and a discussion of cellular sources of oxidants during exercise. We will also discuss the redox modulation of muscle force production/fatigue and address redox-sensitive targets within skeletal muscle. Finally, we will suggest future directions for research in this field. Although this review will focus on a broad range of issues related to exercise-induced oxidative stress, it is impossible for a single report to address all aspects of this expansive field of study. For topics not covered in this report, the interested reader is referred to several reviews on specific aspects of exercise and oxidative stress (8, 27, 75, 90, 92, 106, 112, 164, 168, 179, 181, 216, 252, 302–304,314, 350, 358, 394).

II. MAJOR REACTIVE SPECIES IN CELLS

Although it is a relatively recent discovery, the occurrence of free radicals in biological processes is now widely accepted (71). The nature and properties of the common free radicals, reactive oxygen species, and reactive nitrogen species found in cells will be discussed in this section of the review.

If an atom/molecule contains one or more unpaired electrons and is capable of independent existence, it is referred to as a "free radical"(136). Atoms possess electrons that are usually associated in pairs. Each pair moves in a defined space around the nucleus referred to as the atomic/molecular orbital. One electron of the pair has spin quantum number +1/2 and the other –1/2. When the electrons are in opposite spins, the electronic state is singlet and referred to as "ground state." Electrons with the same spin are "triplet state," but if singlet molecules absorb energy without changing spin, the molecule is in an "excited singlet state" (209). Free radicals can be generated as products of homolytic, heterolytic, or redox reactions, producing either charged or uncharged radical species. Note that reactive oxygen species (ROS) is a general term that refers to not only oxygen-centered radicals but also includes nonradical but reactive derivatives of oxygen (e.g., hydrogen peroxide) (136). Similarly, the term reactive nitrogen species (RNS) refers to both nitrogen radicals along with other reactive molecules where the reactive center is nitrogen. The term reactive oxygen and nitrogen species (RONS) is also used as a collective term for both ROS and RNS and includes both free radical and non-free radical species.

The primary free radicals generated in cells are superoxide (O₂^–) and nitric oxide (NO). Superoxide is generated through either incomplete reduction of oxygen in electron transport systems or as a specific product of enzymatic systems, while NO is generated by a series of specific enzymes (the nitric oxide synthases). Both superoxide and NO are reactive and can readily react to form a series of other ROS and RNS. Thus superoxide dismutates rapidly to form hydrogen peroxide and subsequently hydroxyl radicals in the presence of catalytic transition metals (292) while superoxide and NO rapidly react to form peroxynitrite and subsequently other RNS (69, 292). An overview of major and secondary ROS and RNS follows.

A. Superoxide

Superoxide is primarily formed as an intermediate in biochemical reactions (134). This anion is negatively charged and is relatively membrane impermeable. Nonetheless, compared with other free radicals, superoxide has a relatively long half-life that enables diffusion within the cell and, hence, increasing the number of the potential targets. Inflammatory cells produce relatively large amounts of superoxide as part of the process by which they resist invading organisms (116). Although superoxide is generally considered relatively unreactive compared with other radical species, it can react rapidly with some radicals such as NO and with some iron-sulfur clusters in proteins (136). Interest has surrounded the possibility that protonation of superoxide to produce the hydroperoxyl radical (HO₂·) may occur to at physiological pH, facilitating the transfer of superoxide across membranes (344). Nonetheless, the probability and circumstances required for the protonation of superoxide in cells remains a topic of debate. As a redox active species, superoxide can reduce some biological materials (e.g., cytochrome c) and oxidize others such as ascorbate. Dismutation of superoxide, both spontaneous and catalyzed by the superoxide dismutases, provides a major source of hydrogen peroxide in cells (reaction 1) 

(reaction 1)

B. Hydrogen Peroxide

Hydrogen peroxide (H₂O₂) is a reactive compound that can readily generate free radicals such as the hydroxyl radical in specific circumstances. Hydrogen peroxide is stable, permeable to membranes, and has a relatively long half-life within the cell. Hydrogen peroxide is cytotoxic but is considered a relatively weak oxidizing agent. In addition to formation from dismutation of O₂^–, a number of enzyme systems also generate H₂O₂ including urate and amino acid oxidases. Hydrogen peroxide is unable to oxidize DNA or lipids directly but can inactivate some enzymes (136). The cytotoxicity of hydrogen peroxide primarily occurs through its ability to generate hydroxyl radical through metal-catalyzed reactions, such as the Fenton reaction (reaction 2) 

(reaction 2)

In biology it seems likely that this reaction is particularly important as part of the Haber-Weiss reaction, where iron (or copper) is maintained in a reduced form by superoxide and hence capable of catalyzing the formation of the hydroxyl radical from hydrogen peroxide (134). The net reaction is 

(reaction 2a)

In practice the reactions involved are 

(reaction 2b)

(reaction 2)

or 

(reaction 2c)

(reaction 2d)

C. Hydroxyl Radicals

Hydroxyl radicals (·OH) are highly reactive with a strong oxidizing potential. Hydroxyl radicals damage molecules close to their site of their generation, and due to their high reactivity, they are not membrane permeable. Hydroxyl radicals appear to combine with cell components at a rate constant of 10⁹–10¹⁰M^–1·s^–1 (134). They are potentially the most damaging ROS present in biological materials, and their reactivity is such that it is virtually impossible to confirm‎ that they exist in living organisms other than through demonstration of the presence of specific products of their reactions.

D. Singlet Oxygen

Another reactive form of oxygen, singlet oxygen, can also be generated in some biological materials. Singlet oxygen has a very short half-life but is capable of diffusion and is permeable to membranes. Singlet oxygen is an electronically excited form of oxygen and is not a radical since no electrons are unpaired. Singlet oxygen exists in one of two states, the first excited state (¹ΔgO₂) or the more reactive second excited state (²∑gO₂) (134). Singlet oxygen has no spin restriction, and hence, the oxidizing ability is greatly increased (136). Dismutation of the superoxide anion in water can lead to the formation of singlet oxygen in biological systems. At present, it is unclear if singlet oxygen is produced in contracting skeletal muscles during exercise.

E. Nitric Oxide

Nitric oxide (NO·) is synthesized from the amino acid L-arginine by many cell types. Synthesis occurs through nitric oxide synthases (NOS) of three main types: neuronal NOS (NOS1), which was originally found in neural tissue but is also present in most cell types; endothelial NOS (NOS3), originally described in endothelial cells; and inducible NOS (NOS2) that is predominantly found in inflammatory conditions, but now recognized to be more widespread. The nitric oxide synthases convert L-arginine into NO and L-citrulline utilizing NADPH.

NO readily binds to some transition metals, and its major actions in cells relate to its ability to bind to the ferrous ion in guanyl cyclase activating this enzyme and resulting in the formation of cGMP. This binding of NO to iron not only comprises a major mechanism of action, but also appears to play a major role in its inactivation and removal through binding to the iron in hemoglobin. Nitric oxide is a weak reducing agent, reacts with oxygen to form nitric dioxide, and reacts very rapidly with superoxide to produce peroxynitrite (133).

F. Peroxynitrite

The reaction of superoxide with NO to produce peroxynitrite (reaction 3) occurs approximately three times faster than the dismutation of superoxide to produce hydrogen peroxide and even faster than the reaction of NO with heme proteins; hence, this is the primary reaction when both are present 

(reaction 3)

Peroxynitrite (or its protonated form ONOOH) is a strong oxidizing agent and can lead to depletion of thiol groups, damage to DNA, and nitration of proteins. A further effect of the formation of peroxynitrite is a reduced bioavailability of superoxide and NO. Peroxynitrite is classified as RNS that also includes NO and N₂O₃ and some nitrogen-centered radicals, but much less is known about the occurrence and roles of these latter species in biology.

G. Hyperchlorite

Hyperchlorite is formed by the action of myeloperoxidase utilizing hydrogen peroxide (reaction 4). Hyperchlorite is predominantly formed by neutrophils and can damage various biomolecules by oxidizing thiols, lipids, ascorbate, and NADPH with the generation of various secondary products (136). Moreover, in the acid form (i.e., hypochlorous acid), this oxidant can cross cell membranes and can cause fragmentation and aggregation of proteins by multiple reactions (136) 

(reaction 4)

H. Secondary Radical Species

Since free radicals can initiate radical chain reactions in some biological molecules, a number of other free radical species occur in cells as part of these processes. A primary example of these secondary radical species include the intermediary radicals formed during lipid peroxidation (i.e., oxidative degradation of polyunsaturated lipids). The lipid bilayer is the basic structure of all biological membranes and is composed of lipids and proteins. Polyunsaturated fatty acids (PUFAs) are a large constituent of the membrane around cells and their organelles (136). Lipid peroxidation of PUFAs such as arachidonic acid occurs via several stages. The hydroxyl radical typically initiates peroxidation by abstracting a hydrogen atom from a methylene carbon side chain, initially forming a carbon-centered, lipid radical and water (135). For example, arachidonic acid can undergo hydrogen abstraction at carbons 13, 10, and 7 (136). Monounsaturated and saturated fatty acids have an increased resistance to oxidation compared with PUFAs, since the double bonds in the PUFAs are more susceptible to oxidation (100, 135).

The carbon-centered lipid radical has an unpaired electron, and the molecule stabilizes by molecular rearrangement (393); that is, two lipid radicals can cross-link and form a conjugated diene or usually, the lipid radical donates an electron to oxygen, forming the peroxyl radical (designated LOO·, where L = polyunsaturated fatty acid) and propagating the chain reaction. Generation of a lipid radical is terminated by the formation of a cyclic peroxide or cyclic endoperoxide and other termination products (Fig. 1). Lipid peroxidation can theoretically result in degeneration of membrane structure and loss of membrane protein function (121). Structural derangement of the lipid bilayer alters the fluidity and increases the rigidity of the membrane. This enables proteins within the membrane to be more directly attacked, impairing essential membrane functions such as the activity of intrinsic enzymes and transporters and/or decreasing the rate of carrier coupled flow of ATP and ADP.

View larger version (14K):
In this window
In a new window

 

FIG. 1. Arachidonic acid undergoing initiation and propagation stages of lipid peroxidation. MDA, malondialdehyde. [Adapted from Halliwell and Gutteridge (136).]

III. ANTIOXIDANT DEFENSE SYSTEMS: OVERVIEW AND RESPONSE TO CHRONIC EXERCISE

Later sections of this review will discuss the fact that contracting skeletal muscle fibers can produce ROS via a variety of different pathways (86, 165, 321,325) and outline how ROS are implicated in the etiology of exercise-induced skeletal muscle fatigue (22, 34, 105, 183, 184, 232, 321, 399, 400). Hence, given the importance of maintaining redox homeostasis in muscle fibers, it is not surprising that myocytes contain a network of antioxidant defense mechanisms to reduce the risk of oxidative damage during periods of increased ROS production. The term antioxidant has been defined in many ways, but in the context of our discussion, antioxidants will be broadly defined as any substance that delays or prevents the oxidation of a substrate (i.e., all molecules found in vivo) (136).

To protect against oxidative stress, a well-organized system of antioxidants works in a coordinated fashion to resist redox disturbances in the cell. In this section, we provide an overview of cellular antioxidants and summarize how antioxidants function to protect muscle fibers against oxidative injury. Moreover, we will sum up the literature related to chronic exercise-induced changes in both enzymatic and nonenzymatic antioxidants in skeletal muscle.

A. Cellular Strategies to Regulate Reactive Species

Muscle fibers contain both enzymatic and nonenzymatic antioxidants that work as a complex unit to regulate ROS. Within the fiber, these antioxidants are strategically compartmentalized throughout the cytoplasm and within various organelles (e.g., mitochondria). Moreover, enzymatic and nonenzymatic antioxidants exist in both the extracellular and vascular space. Collectively, these antioxidants protect muscle fibers from oxidative injury during periods of increased oxidant production (e.g., intense or prolonged exercise).

Numerous antioxidant strategies exist and can be used to protect against ROS toxicity. For example, some agents (i.e., catalase) convert ROS into less active molecules and prevent the transformation of these less active species to a more deleterious form. Another antioxidant strategy is to minimize the availability of pro-oxidants such as iron and copper ions via metal binding proteins. Moreover, numerous low-molecular-weight agents are capable of scavenging ROS species. Examples of this antioxidant strategy include endogenously synthesized molecules such as glutathione, uric acid, and bilirubin along with agents found in the diet such as ascorbic acid and vitamin E.

B. Antioxidant Enzymes

Principal antioxidant enzymes include superoxide dismutase, glutathione peroxidase, and catalase (Fig. 2). Additional antioxidant enzymes such as peroxiredoxin, glutaredoxin, and thioredoxin reductase also contribute to cellular protection against oxidation.

View larger version (96K):
In this window
In a new window

 

FIG. 2. Locations of primary cellular enzymatic and nonenzymatic antioxidants. GPX, glutathione peroxidase; SOD1, superoxide dismutase 1; SOD2, superoxide dismutase 2.

1. Superoxide dismutase

Superoxide dismutase (SOD) was discovered in 1969 by McCord and Fridovich (256) and forms the first line of defense against superoxide radicals as SOD dismutates superoxide radicals to form hydrogen peroxide (H₂O₂) and oxygen (O₂). In mammals, three isoforms of SOD (SOD1, SOD2, SOD3) exist, and all require a redox active transition metal in the active site to accomplish the catalytic breakdown of the superoxide anion (82, 385). Two of the SOD isoforms are located within cells, whereas the third SOD isoform is found in the extracellular space (385). SOD1 requires copper-zinc as a cofactor and is primarily located in the cytosol and the mitochondrial intermembrane space. SOD2 uses manganese as a cofactor and is located in the mitochondrial matrix. Finally, SOD3 requires copper-zinc as a cofactor and is located in the extracellular space. A summary of the properties of human SOD isoenzymes is contained inTable 1.

View this table:
In this window
In a new window

 

TABLE 1. Properties of human SOD isoenzymes

Although superoxide radicals are not highly toxic, they can extract electrons from biological membranes or other cellular components, resulting in a chain of radical reactions. Superoxide radicals are also toxic through their ability to participate in the formation of hydroxyl radicals (see sect. IIB) and react with NO to form peroxynitrite (see sect. IIF). Therefore, it is essential for cells to keep superoxide radicals in check. Direct evidence to support this notion is illustrated by the fact that the mutagenesis of SOD1 in humans promotes apoptosis of spinal neurons resulting in amyotrophic lateral sclerosis (115).

The relative allocation of the SOD1 and SOD2 isoenzymes varies across tissues. In skeletal muscle, 15–35% of the total SOD activity is in the mitochondria, and the remaining 65–85% is in the cytosol (182, 299). In rat skeletal muscle, SOD activity is highest in oxidative muscles that contain a high percentage of type I and type IIa fibers compared with muscles with low mitochondrial volumes (i.e., type IIx or IIb fibers) (78, 299).

SOD activity in skeletal muscle is not constant and can be modified by activity patterns. Although some studies suggest that chronic (weeks to months) endurance exercise training does not increase SOD activity in muscle (9, 145, 224, 227), most investigators conclude that endurance exercise training promotes 20–112% increases in the activities of both SOD1 and SOD2 in the exercised muscles (78, 155, 229, 234, 235, 284, 299, 300, 311, 399, 400). Analysis of these individual reports does not provide definitive explanation for the variance between studies. Nonetheless, methodological differences in the assay of SOD activity, variations in the exercise training paradigm, and differences in fiber types between the muscles studied could be contributory factors to the differences reported in the literature. In reference to assay techniques, a 10-fold difference exists in the relative sensitivity between common methods used to assay SOD activity (287). Hence, SOD assay techniques with low sensitivity could fail to detect small-to-moderate group differences in SOD activity, and therefore, this shortcoming could explain the failure to observe exercise-induced increases in SOD activity in some reports. Furthermore, the magnitude of exercise-mediated changes in skeletal muscle SOD activity increases as a function of the intensity and duration of exercise, and therefore, studies exercising animals at higher intensities and/or longer daily durations of exercise typically report a larger percent increase in SOD activity (299,300). Finally, the magnitude of the exercise-induced increase in SOD activity in muscle fibers is greatest in skeletal muscles composed of highly oxidative fibers (e.g., type I and type IIa) (78, 299). The explanation for this blueprint of adaptation could be due to the recruitment patterns of muscle fibers during submaximal endurance exercise whereby highly oxidative fibers are more actively recruited compared with less oxidative fibers (i.e., type IIx and IIB) (342).

2. Glutathione peroxidase

Analysis of the selenoproteome has identified five glutathione peroxidases in mammals (GPX1-GPX5; Table 2) (54, 101). All of these GPX enzymes catalyze the reduction of H₂O₂ or organic hydroperoxide (ROOH) to water (H₂O) and alcohol (ROH), respectively, using reduced glutathione (GSH) or in some cases thioredoxin or glutaredoxin as the electron donor (43, 44, 161). When GSH is the electron donor, it donates a pair of hydrogen ions and GSH is oxidized to glutathione disulfide (GSSG) as follows 

View this table:
In this window
In a new window

 

TABLE 2. Physical characteristics and tissue locations of the multiple GPX proteins in humans

Although the reaction catalyzed by all GPXs appears to be the same, individual GPXs differ in substrate specificity (i.e., varying range of hydroperoxides) and cellular localization (i.e., cytosol vs. mitochondria) (55). The varying substrate specificity and cellular location across GPX isoforms appears to optimize GPX's function as a cellular antioxidant enzyme. Indeed, the fact that many GPX isoenzymes will reduce a wide range of hydroperoxides ranging from H₂O₂to complex organic hydroperoxides makes GPX an important intracellular antioxidant to protect against ROS-mediated damage to membrane lipids, proteins, and nuclei acids (184). Moreover, although all GPX isoenzymes are efficient peroxidases, various GPX isoforms may also exert a variety of specific roles in metabolic regulation such as silencing lipoxygenases (55).

To function, most GPX isoforms require a supply of GSH to provide electrons. Since GSH is oxidized by GPX to form GSSG, cells must possess a pathway capable of regenerating GSH. The reduction of GSSG back to GSH is accomplished by glutathione reductase, a flavin containing enzyme whereby NADPH provides the reducing power (263). Many tissues produce NADPH by glucose-6-phosphate dehydrogenase via the pentose pathway, but skeletal muscles produce NADPH primarily via isocitrate dehydrogenase (228, 230, 262).

Identical to SOD, the relative amount of GPX present in skeletal muscle fibers differs across fiber types; that is, highly oxidative fibers (i.e., type I) contain the highest GPX activity, whereas rodent muscle fibers with low oxidative capacity (i.e., type IIB) possess the lowest levels of GPX (231, 299). Identical to SOD, GPX is inducible in skeletal muscles and GPX is reported to increase in skeletal muscle fibers that are actively recruited during regular exercise. Indeed, numerous studies confirm‎ that endurance exercise training promotes an increase (20–177%) in GPX activity in skeletal muscles (78, 145, 182,197, 224, 229, 234, 235, 299, 300, 307, 352, 397, 398). Moreover, endurance exercise increases both cytosolic and mitochondrial GPX activity (182). Similar to SOD, the extent of the exercise-induced increase in GPX in skeletal muscle is a function of both the exercise intensity and exercise duration. Specifically, compared with low-intensity exercise, both moderate- and high-intensity exercise produce a larger increase in muscle GPX activity (299). Moreover, long-duration exercise training sessions (e.g., ≥60 min/day) are superior to short-duration (≤30 min/day) exercise bouts in increasing muscle GPX activity (299). Finally, exercise training-induced increases in muscle GPX activity are typically limited to highly oxidative muscles containing primarily type I and IIa fibers (78, 299). Similar to the aforementioned scenario with exercise-induced induction of SOD, the explanation for this adaptive pattern of GPX is likely due to the fact that, compared with fast type IIX or IIb fibers, highly oxidative muscle fibers (i.e., type I) are more actively recruited during submaximal endurance exercise (342).

3. Catalase

Catalase (CAT) serves several biochemical functions, but the principal purpose of CAT is to catalyze the breakdown of H₂O₂ into H₂O and O₂ 

Catalase is a homotetramer with a molecular mass of 240 kDa and is widely distributed within the cell (206). Iron is a required cofactor attached to the active site of the enzyme (64, 206, 416). Although CAT and GPX share common substrates, CAT has a much lower affinity for H₂O₂ at low concentrations compared with GPX (i.e., GPX K_m = 1 μM vs. CAT K_m = 1 mM) (363).

CAT protein levels are highest in highly oxidative muscle fibers and lowest in fibers with low oxidative capacity (227, 234, 293, 299). Whether or not CAT expression‎ in skeletal muscle increases in response to chronic exercise is controversial with studies reporting an increase (311, 400), decrease (227, 234,299), or no change (299) in muscle CAT activity following training. The ambiguity of these findings may be due to a variety of factors including issues associated with assaying CAT activity. For example, CAT activity assays typically involve the addition of its substrate (i.e., H₂O₂), and the degradation of H₂O₂ is followed spectrophotometrically. However, interestingly, the V_max of catalase increases as a function of the concentration of H₂O₂ present in the assay medium (184). Therefore, using this technique, the assessed CAT activity is dependent on not only the amount of active CAT protein in the assay medium, but also the concentration of H₂O₂ used as substrate (178). Hence, failure to carefully standardize the conditions of the assay may contribute to the wide variation of CAT activities reported within and between studies.

4. Accessory antioxidant enzymes

In addition to the aforementioned primary antioxidant enzymes, cells contain several other enzymes that can contribute directly or indirectly to the maintenance of redox balance. Among these, the thioredoxin, glutaredoxin, and peroxiredoxin systems are important contributors. The thioredoxin antioxidant system is composed of thioredoxin (TRX) and thioredoxin reductase (25, 41, 161, 413). The mammalian TRX is a highly conserved 12-kDa protein and cells contain two TRX systems, the cytosolic (TRX1) and the mitochondrial (TRX2) (41). Functionally, TRX is the major ubiquitous disulfide reductase responsible for maintaining proteins in their reduced state (25). Oxidized TRX is then reduced by electrons from NADPH via thioredoxin reductase (159). Along with the prevention of protein oxidation, numerous other physiological functions of TRX have been described including reduction of transcription factors, protection against oxidative stress, and control of apoptosis (25). Moreover, thioredoxin reductase also contributes as an antioxidant enzyme by reducing hydroperoxides and functioning as a NADPH-dependent dehydroascorbate reductase to recycle vitamin C (25).

Similar to TRX, glutaredoxin (GRX) is a thiodisulfide oxidoreductase that is involved in the protection and repair of protein and non-protein thiols during periods of oxidative stress (41, 158). GRX protects thiols by the transfer of electrons from NADPH to disulfide substrates, and this catalytic cycle is coupled with glutathione and glutathione reductase (41). Human cells contain three different GRXs; GRX1 is located in cytosol, whereas both GRX2 and GRX5 are located in the mitochondria (122, 240, 407).

Although TRX and GRX both control the redox state of thiol groups of cysteinyl side chains, their simultaneous presence in cells suggests different functions for each protein (248). Indeed, while TRX and GRX have some overlapping functions, GRXs are uniquely reactive with glutathione-mixed disulfides (160). Hence, it appears that GRX and TRX function in a cooperative manner to maintain the reduced redox state of both protein and non-protein thiols (374).

Peroxiredoxin (PRX) was discovered in 1988 and is a novel peroxidase capable of reducing both hydroperoxides and peroxynitrate with the use of electrons provided by a physiological thiol like TRX (204, 205, 327). Mammalian cells express six isoforms of PRX (PRX I–VI) that are distributed differentially within the cell: PRX I, II, and VI are found in the cytosol; PRX III is located in the mitochondrion; PRX IV is located in the extracellular space; and PRX V is located in both mitochondria and peroxisomes (327). Unfortunately, a kinetic analysis of PRX reaction rates at physiological concentrations of substrates has not been reported for any of the PRX isoforms. Nonetheless, the molar efficiencies of PRXs are generally smaller than GPX or CAT by several orders of magnitude (113). Therefore, although PRXs may defend against cellular oxidative stress, the importance of their antioxidant role in mammalian cells remains unclear (113). Moreover, growing evidence suggests that in addition to antioxidant properties, these peroxidases may also play a role in the regulation of H₂O₂ as a second messenger in receptor-mediated signaling (192, 328, 329).

Finally, the effects of regular exercise on the TRX, GRX, and PRX systems in skeletal muscles remain unknown. Nonetheless, it is conceivable that exercise-induced changes in one or all of these antioxidant systems could contribute to the redox adaptation to exercise. This topic remains an interesting area for future work.

C. Nonenzymatic Antioxidants

Numerous nonenzymatic antioxidants exist in cells (e.g., GSH, uric acid, bilirubin, etc.), and a detailed discussion of this topic is beyond the scope of this review. Nonetheless, in this segment, we will provide a brief overview of selected nonenzymatic antioxidants located within skeletal muscle fibers. For a detailed discussion of nonenzymatic antioxidants, the reader is referred to numerous published reviews (97, 123, 177, 178, 184, 298, 303, 408).

Clearly, one of the most important nonenzymatic antioxidants in muscle fibers is GSH. Glutathione is a tripeptide and is the most abundant nonprotein thiol in cells (263). This antioxidant is primarily synthesized in the liver and transported to tissues via the circulation. Glutathione concentration in cells is in the millimolar range for most tissues, and GSH content varies across organs depending on their function (263). For example, tissues with high exposure to oxidants (e.g., lens of the eye and liver) contain high levels of GSH. Similarly, the concentration of GSH found in skeletal muscle fibers varies across fiber types with type I fibers in rats containing 400–600% higher GSH content (i.e., 2–3 mM) compared with type IIb fibers (i.e., ∼0.5 mM) (235).

As an antioxidant, GSH serves multiple roles in cells. First, GSH can directly react with a variety of radicals by donating a hydrogen atom (414). Second, as mentioned earlier, one of the key antioxidant actions of GSH is to serve as a substrate for GPX to eliminate H₂O₂ and organic hydroperoxides (263). Furthermore, GSH is also involved in reducing other antioxidants in the cell including vitamins E and C. In this regard, GSH has been shown to reduce vitamin E radicals that are formed in chain-breaking reactions with alkoxyl or lipid peroxyl radicals. Similarly, GSH can reduce the semidehydroascorbate radical (vitamin C radical) derived from the recycling of vitamin E. Collectively, these GSH-mediated reactions assist in maintaining the limited resources of vitamin E and C in the reduced state at the expense of GSH (184).

Studies indicate that skeletal muscle fibers adapt to regular bouts of high-intensity endurance exercise by increasing the levels of GSH (234, 235, 246,285, 352). It has been suggested that the exercise-induced increase in GSH within muscle fibers is due to increased activity of a key enzyme involved in GSH synthesis (178). Specifically, the activity of the rate-limiting enzyme for GSH biosynthesis (i.e., γ-glutamylcysteine synthase) is increased in exercise-trained muscles and probably plays an important role in exercise-induced increases in GSH synthesis in skeletal muscle (221, 246, 352).

During the past 15 years, α-lipoic acid has received much attention as a cellular antioxidant. α-Lipoic acid is a naturally occurring compound and can be obtained in the diet from a variety of foods (189, 289, 291, 335). Functionally, α-lipoic acid serves as a cofactor for α-dehydrogenase complexes and participates in S-O transfer reactions (189, 289). Normally, α-lipoic acid is present in very small quantities in animal tissues and is generally bound to an enzyme complex which limits its function as an antioxidant (291). However, unbound and reduced α-lipoic acid (dihydrolipoic acid) and several of its metabolites are effective antioxidants (189, 291). Furthermore, numerous studies support the notion that α-lipoic acid can provide beneficial antioxidant effects in a physiological context and can play a role in recycling vitamin C (24, 46, 62, 72, 117, 189, 291, 412, 423). Although an acute bout of exercise appears to increase α-lipoic acid levels in skeletal muscle, chronic exercise training does not alter muscle levels of α-lipoic acid (200).

Uric acid is a by-product of purine metabolism in humans and other primates and is potentially an important low-molecular-mass antioxidant in human biological fluids (14, 148). At physiological pH, almost all uric acid is converted to urate (365). The antioxidant role of uric acid/urate was first reported in 1960, and additional evidence for its antioxidant properties grew in the 1980s by experiments demonstrating that uric acid was a powerful scavenger of peroxyl radicals, hydroxyl radicals, and singlet oxygen (14, 87, 162, 356). As an antioxidant, urate is able to protect against oxidative damage by acting as an electron donor (136). Urate is also able to chelate metal ions such as iron and copper and prevent them from catalyzing hydroxyl radicals via the Fenton reaction (136). The impact of chronic exercise on muscle urate levels remains unknown, but it appears that urate could be an antioxidant scavenger in skeletal muscle during exercise (147, 148).

Bilirubin is the final product of hemoprotein catabolism as heme oxygenase cleaves the heme ring to form biliverdin; biliverdin is then reduced by biliverdin reductase to form bilirubin (376). Although both biliverdin and bilirubin are reducing species, bilirubin is considered to be the best physiological antioxidant (33). Indeed, bilirubin possesses strong antioxidant potential against peroxyl radicals and has been shown to protect cells from toxic levels of hydrogen peroxide (33, 377, 378). It has been suggested that the powerful physiological antioxidant actions of bilirubin are a result of an amplification cycle whereby bilirubin acting as an antioxidant, is itself oxidized back to biliverdin and then recycled back to bilirubin via biliverdin reductase (33). Although intense exercise increases blood levels of bilirubin (111, 258), the impact of chronic exercise on bilirubin levels in skeletal muscle remains unknown.

Another nonenzymatic antioxidant worthy of discussion is coenzyme Q₁₀. Conenzyme Q₁₀ (ubiquinone) is synthesized in cells and is essential in mitochondrial electron transport and is also located in cell membranes (136). In vitro, coenzyme Q₁₀ can function as a nonenzymatic antioxidant by scavenging RO· radicals and inhibiting lipid peroxidation. Nonetheless, the contribution of coenzyme Q₁₀ to antioxidant defense in vivo remains uncertain. Although several studies have investigated the effects of coenzyme Q₁₀ supplementation on skeletal muscle function during exercise (146, 190, 264, 334,359, 386), the impact of endurance exercise training on coenzyme Q₁₀ levels in muscle remains relatively unknown (84).

D. Dietary Antioxidants

Numerous dietary antioxidants may also contribute to cellular protection against radicals and other ROS. Important dietary antioxidants include vitamin E, vitamin C, and carotenoids. Vitamin E is one of the most widely distributed antioxidants in nature, and it is the primary chain-breaking antioxidant in cell membranes (171, 290). The generic term vitamin E refers to at least eight structural isomers of tocopherols or tocotrienols (171, 347). Among these, α-tocopherol is the best known and possesses the most antioxidant activity (171). In addition to its direct antioxidant properties, growing evidence suggests that some of the beneficial effects of vitamin E in cells resides in its ability to regulate gene expression‎ of proteins (30, 31, 139, 349).

Several studies have investigated the effects of acute and chronic exercise on vitamin E levels in skeletal muscles of rodents. Unfortunately, the results are not consistent with some studies reporting an exercise-induced decrease in muscle vitamin E concentration (49, 125), whereas others conclude that both acute and chronic muscular activity does not alter muscle vitamin E levels (74, 343, 375). Finally, studies investigating the impact of chronic exercise on vitamin E levels in human skeletal muscle suggest that exercise does not alter vitamin E levels (389, 390).

Similar to vitamin E, carotenoids (e.g., β-carotene) are lipid-soluble antioxidants located primarily in the membranes of tissues. The antioxidant properties of carotenoids come from their structural arrangement consisting of long chains of conjugated double bonds; this arrangement permits the scavenging of several different ROS species including superoxide and peroxyl radicals (108, 222, 372). Because of their cellular location and their radical scavenging capacity, carotenoids are efficient biological antioxidants against lipid peroxidation (222). To date, the effects of chronic exercise on muscle levels of carotenoids have not been investigated.

In contrast to both vitamin E and the carotenoids, vitamin C (ascorbic acid) is hydrophilic and functions better in an aqueous environment. Because the pK_aof ascorbic acid is 4.25, the ascorbate anion is the predominant form existing at physiological pH (414). Ascorbate is widely distributed in mammalian tissues, and its role as an antioxidant is twofold. First, vitamin C can directly scavenge superoxide, hydroxyl, and lipid hydroperoxide radicals (63). Second, vitamin C plays an important role in the recycling of vitamin E, a process that results in the formation of a vitamin C (semiascorbyl) radical (288). Nonetheless, this semiascorbyl radical can be reduced back to vitamin C by NADH semiascorbyl reductase, or cellular thiols such as glutathione or dihydrolipoic acid (290).

IV. OXIDATIVE STRESS

The term oxidative stress was first defined in 1985 as "a disturbance in the pro-oxidant-antioxidant balance in favor of the former" (363, 364). Although this definition has been widely used for over two decades, the definition of oxidative stress will likely evolve and undergo modification in the future. Indeed, because of the complexity associated with the assessment of cellular redox balance, it has been argued that the term oxidative stress defies a simple pro-oxidant versus antioxidant definition and that the description of an "oxidant stress" is only useful if the molecular details of the imbalance are known (29,186). In an effort to refine the meaning of oxidative stress, Dean Jones has proposed that this term should be redefined as "a disruption of redox signaling and control" (186). Regardless of whether this new definition gains widespread acceptance, it can be anticipated that the description of oxidative stress will undergo future modifications as the field of redox biology advances.

Regardless of how oxidative stress is defined, a persistent pro-oxidant environment in cells can modify redox-sensitive molecules. A common approach to assess oxidative stress in biological systems involves the measurement of the increase or decrease in a redox-sensitive molecule that responds to oxidative stress. In general, reliable markers of oxidative stress possess the following qualities: 1) chemically unique and detectable, 2) increased or decreased during periods of oxidative stress, 3) possess relatively long half-lives, and 4) not impacted by other cellular processes (e.g., cell cycle, energy metabolism, etc.) (136).

Many molecules that fit one or more of these criteria have been identified, and techniques to measure these biomarkers have been reported (88, 99, 138,163, 186, 233, 331, 338). During periods of oxidative stress, pro-oxidants overwhelm the antioxidant defenses in cells and damage cellular constituents. Thus oxidative stress in biological systems is often characterized by the following parameters: 1) increase in the formation of radicals and other oxidants,2) decrease in small-molecular-weight and/or lipid-soluble antioxidants, 3) disturbance in cellular redox balance, and 4) oxidative damage to cellular components (i.e., lipids, proteins, and/or DNA). Hence, biomarkers of oxidative stress typically fall into one of four categories (Fig. 3). The first category of biomarkers involves the detection of oxidants. Unfortunately, direct measurement of radical production in living cells is difficult because radicals are highly reactive and have a short half-life. Therefore, exogenous molecules such as fluorescent probes or spin traps are commonly used to measure oxidant production in cells. When added to a biological system, the probe or spin trap is converted into a unique modified radical product with a relatively long half-life that can be quantified as a measurement of oxidant production (138, 163). Nonetheless, because an increase in oxidant production does not necessarily define a pro-oxidant condition, measures of increased oxidant production alone are not definitive markers of oxidative stress. Moreover, a potential disadvantage of using probes or spin traps is that these molecules may perturb the biological system being investigated (136). Another drawback with this approach is that many spin traps and fluorescent probes are toxic to cells (136).

View larger version (21K):
In this window
In a new window

 

FIG. 3. The four broad classes of biomarkers used to assess cellular oxidative stress in tissues. These categories include the measurement of oxidant production, cellular levels of antioxidants, oxidation products, and the antioxidant/pro-oxidant balance. 8-OH-dG, 8-hydroxydeoxyguanosine; GSH/GSSG, ratio of reduced glutathione to oxidized glutathione.

A second group of oxidative stress biomarkers incorporates the measurement of antioxidants in tissues. In theory, decreases in antioxidants (e.g., glutathione, ascorbate, α-tocopherol, etc.) are common occurrences during oxidative insults, and therefore, measurement of a decline in tissue antioxidant levels has been used as a biomarker of oxidative stress. Although assessment of tissue antioxidant levels has merit as a biomarker of oxidative stress, this approach is not without weaknesses. For example, other factors such as changes in cellular metabolism and diet can influence antioxidant levels in cells. Another concern associated with the measurement of tissue antioxidants is the potential for auto-oxidation during sample handling resulting in antioxidant depletion in the tissue (136).

A third class of biomarkers of oxidative stress involves the eval‎uation of oxidatively modified molecules. Indeed, ROS attack of lipids, protein, or DNA generates uniquely oxidized biomolecules that can be used as "fingerprints" to detect oxidative stress in cells. Common measures of bio-oxidation include the measurement of protein carbonyls as an indicator of protein oxidation; assessment of isoprostanes, malondialdehyde, and 4-hydroxyl-2-nonenol as signs of lipid peroxidation; and eval‎uation of DNA oxidation by assaying the levels of the oxidized base, 8-hydroxy-2'-deoxyguanosine (8-OH-dG) (88,163, 198, 233). Although it can be argued that oxidized molecules are the most important biomarker of oxidative stress, their measurement in biological systems is often difficult because oxidized molecules exist in limited amounts in cells even during periods of oxidative stress. Moreover, identical to other biomarker methodologies, measurement of oxidation products is subject to assay artifacts if tissue samples are handled improperly.

The fourth and final category of oxidative stress biomarkers involves the measurement of cellular redox balance. One of the most commonly measured markers of cellular redox balance is the ratio of GSH to GSSH. This assay is useful because increased oxidant production results in a decrease in the GSH/GSSH ratio, indicating lower levels of reduced GSH in favor of increased oxidized GSH (i.e., GSSG). Nonetheless, although this assay is conceptually simple, experimental artifacts are common and can occur during tissue removal and sample processing due to improper tissue handling permitting auto-oxidation (136).

In summary, numerous approaches to assess oxidative stress in biological systems have been reported in the literature. Unfortunately, each category of oxidative stress biomarkers has limitations. Therefore, although there are many parameters to quantify oxidative stress, the development of a single and ideal biomarker has proven to be a difficult task. Hence, it appears that no one biomarker best assesses oxidative stress and that in most cases, the measurement of multiple biomarkers is required to confirm‎ the presence of oxidative stress in tissues (136).

V. HISTORICAL OVERVIEW OF EXERCISE-INDUCED OXIDATIVE STRESS

Although Commoner et al. (71) recognized that cells contained free radical intermediates in the 1950s, the first suggestion that exercise was associated with an increase in the formation of lipid peroxidation by-products did not appear until the late 1970s. This section will briefly describe the directions that the field took over the ensuing 30 years and identify several of the key scientists involved. Inevitably this historical account is written from our personal points of view, and hence, we apologize to colleagues who completed studies that contributed to this field but have been inadvertently omitted.

Using the techniques available in the 1970s to analyze lipid peroxidation, Brady et al. (51) and Dillard et al. (98) reported increased lipid peroxidation during exercise in both rats and humans. These data were subsequently confirmed and expanded by Kelvin Davies and colleagues working in Lester Packer's laboratory at the University of California-Berkeley (86). This 1982 paper is commonly cited as the first evidence that contracting skeletal muscles produce free radicals and that ROS production during exercise is potentially damaging to tissues. The role of mitochondria in generation of superoxide and hydrogen peroxide had been originally reported by researchers in Britton Chance's laboratory in the 1970s (48) and appeared to provide a ready explanation for the source of radical production in skeletal muscle reported by Davies et al. (86).

During the early 1980s, Lester Packer's laboratory investigated the role of antioxidant nutrients in the protection of cells and organelles from radical-mediated oxidative damage (85, 313). A summary of these ground-breaking studies from the Packer group was reported at a Ciba Foundation Symposium in London in 1983 (312). At this same meeting, Jackson and colleagues from University College (London) also presented their contemporary studies examining the role of free radicals as damaging agents to muscle and the possible beneficial effects of vitamin E in reducing exercise-induced damage (167). Other work by Jackson and colleagues focused on the role of calcium influx in degradative processes in muscle (166, 185). This research was complimented by studies indicating that depletion of the antioxidant vitamin E from rats increased the risk of contraction-induced membrane damage in skeletal muscles (165, 167). These results complemented earlier reports describing muscle degeneration in vitamin E-deficient animals (86). Collectively, these studies stimulated interest in many laboratories to investigate whether vitamin E (and subsequently other antioxidant nutrients) could retard both tissue damage and muscle contractile dysfunction that occurred during some forms of muscular exercise. These studies have continued to the present day. Nonetheless, although some reports have concluded that vitamin E supplementation decreases the markers of oxidation in tissues (e.g., Refs. 126, 195,223), positive effects of dietary antioxidants against contraction-induced muscle damage and muscular fatigue are not commonly observed. Moreover, the enthusiasm for further research in this area has also undoubtedly been influenced by the disappointing results of large-scale intervention studies using high doses of antioxidants in many chronic disorders (6, 315).

The techniques used to examine free radical production in skeletal muscles in the early studies utilized the approaches available at that time, mainly measurements of the products of free radical reaction with lipids (51, 98). However, Davies et al. (86) and Jackson et al. (165) also utilized electron-spin resonance spectroscopy (ESR) to examine the relatively stable species that is observed by direct ESR analysis of tissues. Subsequent studies examined other measures of ROS activity, but the elucidation of which free radical species are generated by contracting skeletal muscle occurred only after a further ∼10 yr of research. Specifically, ensuing work revealed superoxide release from the contracting diaphragm (321, 322, 325), the demonstration of NO generation by skeletal muscle (32, 207), and the detection of hydroxyl radical formation by contracting muscle tissue (94, 280).

As mentioned previously, the key antioxidant enzyme SOD was characterized in 1969 by Joe McCord and Irwin Fridovitch. The role of SOD and other antioxidant enzymes in regulation of free radical activity during exercise and the variation in tissues during and following exercise were investigated by numerous investigators in the 1980s (10, 155, 173, 194). The dramatic ability of the body to increase antioxidant capacity with acute and chronic exercise was subsequently described in detail in heart, respiratory, and locomotor skeletal muscles. Key contributors to this work were Li Li Ji and colleagues at the University of Wisconsin (175, 180, 182) and Scott Powers and colleagues at the University of Florida (78, 137, 232, 299–301).

Subsequent studies identified the key role that glutathione plays in regulating free radical activity in exercising subjects. A key contributor to this work was Chandan Sen and colleagues who described the effects of varying levels of glutathione on exercise responses and the effects of exercise on glutathione content and redox status (351, 352). Jose Vina and colleagues from the University of Valencia (Spain) also contributed significantly to this area (345) and built on the expertise developed by Jose Vina during fundamental work on glutathione metabolism undertaken with Sir Hans Krebs in Oxford, UK.

The recognition that contraction-induced free radicals can influence muscle function and fatigue can be attributed to several investigators including Gerald Supinski, Michael Reid, Jack Barclay, and their colleagues as much of this work was published in the early 1990s (34, 321, 322, 325, 361). This initial work set the stage for subsequent studies to explore redox-sensitive targets in skeletal muscle. Specific details regarding the free radical influence on skeletal muscle function will be addressed in detail in a subsequent segment of this review.

Chandan Sen and Lester Packer also played a key role in highlighting the potential job that free radicals play in modulating cell signaling processes (353) and helped initiate the interest in redox-signaling that continues to the current day. This work paved the way to the increased realization that free radicals play a crucial role in activating degradatory pathways leading to loss of muscle mass that has been developed in studies of diaphragm muscle fatigue and degeneration by Gerald Supinski (360, 361, 382), Thomas Clanton (94, 96), and others (211–215, 255, 357).

Thus, during a period of <30 yr, the field of "exercise" redox biology moved from relatively simple studies that examined changes in markers of "oxidative damage" and their prevention to the current status of a growing understanding of the "redox biology" of skeletal muscle and exercise. Most importantly, instead of being considered a peripheral area of exercise physiology, redox signaling in contracting skeletal muscle is now viewed as a basic element in exercise biology. Inevitably this brief historical review has excluded many individual papers since the field has expanded dramatically within the last 30 yr. A brief examination of the number of papers published in the area of free radicals and exercise indicates that in 1982–1983, 10 papers were published, compared with almost 1,000 reports in 2006, and this growth of the literature shows no sign of decline.

VI. SOURCES OF RADICALS DURING EXERCISE

There are many potential tissue sources from which ROS and RNS may be produced during exercise, but surprisingly few studies have investigated the predominant tissues responsible for this production. This is likely due to both the restricted access to most tissues in humans and the complex nature of exercise that involves many organ systems that are linked through the increased metabolic requirement of skeletal muscles. Hence, although many studies have examined whole body indicators of free radical activity [such as the release of expired pentane originally reported by Dillard et al. (98)], the majority of these reports have assumed that skeletal muscle provides the major source of free radical and ROS generation during exercise. It is however entirely feasible that in some situations other tissues such the heart, lungs, or white blood cells may contribute significantly to the total body generation of ROS.

Because of the invasive nature of obtaining tissue samples from exercising humans, many studies have examined whole body indicators of oxidation. Despite the many variations in the exercise model examined, many studies have confirmed that increased lipid oxidation (e.g., Ref. 26), DNA oxidation (e.g., Ref. 405), and oxidation of other components (e.g., Ref. 355) can be observed in blood, although inevitably this has not been observed by all authors (e.g.. see Ref. 339). Some authors have suggested that common metabolic changes that occur during most exercise protocols such as the increased release of catecholamines may play a role in the increased ROS generation (75), but the general consensus has been that ROS generation occurs predominantly by contracting skeletal and heart muscle. An exception to this rule is an experiment whereby muscle damage occurs, and in this situation, inflammatory processes may play an important role in radical production. In the following three segments, we will discuss several potential production sites of superoxide radicals and NO along with sources of ROS production in muscle following damage.

A. Endogenous Sites for Superoxide Generation in Skeletal Muscle

Skeletal muscle generates superoxide at multiple subcellular sites, several of which increase in activity during muscle contractions. Numerous potential sites for superoxide production exist in skeletal muscle and are summarized in the following segments (Fig. 4).

View larger version (37K):
In this window
In a new window

 

FIG. 4. Potential sites for the production of superoxide and nitric oxide in skeletal muscle.

1. Mitochondria

Mitochondria have generally been cited as the predominant source of ROS in muscle cells (e.g., Refs. 85, 217), and many authors have reiterated early reports that 2–5% of the total oxygen consumed by mitochondria may undergo one electron reduction with the generation of superoxide (48, 239). More recent research has identified the major site(s) of superoxide generation within mitochondria, and most data now indicate that complexes I and III of the electron transport chain are the main sites of mitochondrial superoxide production (36, 272). In complex I, the main site of electron leakage to oxygen appear to be the iron-sulfur clusters, and in complex III, it appears to be the Q₁₀ semiquinone (272). Furthermore, complex III releases superoxide from both sides of the inner mitochondrial membrane (272). It is unclear if this superoxide crosses the outer mitochondrial membrane or is dismutated by Cu/Zn-SOD located in the mitochondrial intermembrane space where it may minimize this possibility. During exercise, a number of researchers have assumed that the increased ROS generation that occurs during contractile activity is directly related to the elevated oxygen consumption that occurs with increased mitochondrial activity, implying potentially a 50- or 100-fold increase in superoxide generation by skeletal muscle during aerobic contractions (e.g., see Refs. 193, 392). However, a recent finding suggests that mitochondria may not be the dominant source of ROS during exercise (170), and future studies will be required to fully elucidate the role that mitochondria play in contraction-induced production of ROS in skeletal muscle.

Brand and colleagues (371) have recently reassessed the rate of production of ROS by mitochondria and have concluded that the upper estimate of the total fraction of oxygen utilized that forms superoxide was ∼0.15%; this value is several orders of magnitude lower than the original estimate of 2–5% (371). This low rate of superoxide production may include a role for uncoupling proteins (specifically UCP3 in skeletal muscle) as regulators of mitochondrial production of ROS (52, 53) acting to protect mitochondria against oxidative damage. In addition, there has been considerable debate about the effect of changes in the respiratory state on ROS generation by mitochondria, and growing evidence reveals that mitochondria produce more ROS during state 4 (basal) respiration compared with state 3 (maximal ADP-stimulated respiration) (4, 93, 149, 219). This is significant because during aerobic contractile activity, skeletal muscle mitochondria are predominantly in state 3, and this limits their generation of ROS during contractions (93, 149, 219). Collectively, these findings suggest that mitochondria are not the primary source of ROS production in skeletal muscle during exercise.

Finally, recent evidence suggests that compared with type I fibers, type II skeletal muscle fibers possess unique properties that promote mitochondrial ROS production. Specifically, using an in situ approach to measure H₂O₂ release from mitochondria in permeabilized rat muscle fiber bundles, a recent study reported that mitochondrial ROS leak (i.e., H₂O₂ release/O₂ consumed) was two- to threefold greater in type II fibers compared with type I (15). The mechanism responsible for this observation remains unclear but does not appear to be due to differences in mitochondrial GPX activities between type I and II fibers.

2. Sarcoplasmic reticulum

Studies have identified NAD(P)H oxidase enzymes associated with the sarcoplasmic reticulum (SR) of both cardiac (66) and skeletal muscle (409). The superoxide generated by these enzymes appears to influence calcium release by the SR through oxidation of the ryanodyne receptor (66). The skeletal muscle enzyme described appears to preferentially use NADH as substrate (409). Some inhibitor studies have indicated that extracellular superoxide release from stimulated myotubes was reduced by treatment with diphenyleneiodonium (DPI), a nonspecific inhibitor of NAD(P)H oxidases (292), although the NADH oxidase described by Xia et al. (409) is localized to the SR and hence seems unlikely to contribute to the extracellular release.

3. Transverse tubules

Recent data indicate that the transverse tubules of skeletal muscle contain a NADP(H) oxidase whose activity is increased by depolarization (109, 154). This enzyme contains some of the classical subunits found in the NADP(H) oxidase of phagocytic cells and appears to release superoxide to the cytosol of skeletal muscle cells. Finally, this skeletal muscle specific NADP(H) oxidase is inhibited by nonspecific inhibitors of this class of enzyme and by prevention of membrane depolarization.

4. Plasma membrane

Numerous studies reveal that skeletal muscle cells release superoxide into the extracellular space (e.g., see Refs. 253, 292, 321, 325, 427). All cells contain plasma membrane redox systems capable of undertaking electron transfer across the plasma membrane. A NAD(P)H oxidase complex has been reported to be constitutively expressed in diaphragm and limb muscles of the rat and localized to the region of the plasma membrane (172). The enzyme contains four of the subunits that are found in the enzyme in phagocytic cells (gp91^phox, p22^phox, p47^phox, and p67^phox), all of which were associated with the cell membranes (236). Whether this complex predominantly releases superoxide to the inside or the outside of the plasma membrane cannot be ascertained from the experiments reported (172), and any potential overlap with the recent discovery of the NAD(P)H oxidase located in the transverse tubules (109, 154) has not been investigated.

There are other plasma membrane redox systems that are capable of transferring electrons from intracellular reductants to appropriate extracellular electron acceptors, although no such system has been described in skeletal muscle (346). Morre (269) has described external NADH oxidase (ECTO-NOX) proteins that exhibit a hydroquinone (NADH) oxidase activity and a protein disulfide-thiol exchange activity. The current understanding of these systems is that they accept electrons from the hydroquinones of the plasma membrane and can reduce a number of nonphysiological (e.g., ferricyanide and WST-1) and physiological (e.g., protein thiols or oxygen) electron acceptors outside the cell, although oxygen is likely to be a major acceptor in vivo (89). Transfer of electrons from cytosolic NAD(P)H to the plasma membranes has been proposed to occur through either NADH-cytochrome b₅ oxidoreductase or NAD(P)H quinone oxidoreductase (NQO1) (89). Thus, through a series of linked steps, intracellular NAD(P)H can act as substrate for superoxide generation on the cell surface. The relevance of these processes to skeletal muscle contractions has not been established, but it is feasible that these systems are activated during contractile activity or that the substrate level rises to increase electron transfer across the membrane through these systems. The characteristics of the release of superoxide from skeletal muscle are compatible with the involvement of such a system.

5. Phospholipase A₂-dependent processes

Phospholipase A₂ (PLA₂) is an enzyme that cleaves membrane phospholipids to release arachidonic acid, which is a substrate for ROS-generating enzyme systems such as the lipoxygenases (426). Also, activation of PLA₂ can stimulate NAD(P)H oxidases (421), and increased PLA₂ activity has been reported to stimulate ROS generation in muscle mitochondria (276) and cytosol (130) and release ROS into the extracellular space (426). Both calcium-dependent and independent forms of PLA₂ are reported to play a role in muscle ROS generation. The calcium-independent enzymes (iPLA₂) have been claimed to modulate cytosolic oxidant activity in skeletal muscle cells (130), while a 14-kDa calcium-dependent isoform (sPLA₂) located within mitochondria has been reported to stimulate intracellular ROS generation during contractile activity (277). In nonmuscle cells, activity of the third major type of PLA₂, cytosolic (cPLA₂) that is activated by micromolar concentrations of calcium, has been linked to ROS generation (273). Reid and colleagues (130) hypothesized that the calcium-independent PLA₂ was a major determinant of ROS activity under resting conditions, whereas during contractions, heat stress, or other processes elevating intracellular calcium, the calcium-dependent PLA₂ was activated and stimulates ROS production at supranormal rates.

6. Xanthine oxidase

There has been considerable speculation concerning a role for xanthine oxidase in superoxide generation by skeletal muscle. At present, this speculation is primarily based on the effects of the xanthine oxidase inhibitors allopurinol or oxypurinol (e.g., see Refs. 129, 151). Although rat skeletal muscles contain significant levels of xanthine oxidase (188), human skeletal muscle cells per se appear to possess low amounts of xanthine dehydrogenase or oxidase (145), although these enzymes will inevitably be present in associated endothelial cells. Clearly, additional research is required to determine the role that xanthine oxidase plays in exercise-induced ROS production.

Thus there is clear evidence that superoxide and hydrogen peroxide are generated in muscle cells during contractions, and more limited data indicate that hydroxyl radicals may be generated under more specific circumstances. Despite the initial indications that mitochondria are the predominant site for ROS generation during activity, a number of alternative potential sites have been identified. It is still unclear whether all of these multiple sites contribute to the increased ROS activities that are observed during contractions or whether one site predominates. It is entirely feasible that the multiple sites of generation are active in differing situations and that the effects of the ROS generated are relatively localized and important for disparate functions. For example, localized ROS generation by the sarcoplasmic reticulum or t-tubule systems may be much more important in regulation of sarcoplasmic reticulum calcium handling (see sect. VIIIA1) than ROS generated by mitochondria or extracellular oxidases.

B. Endogenous Sites for NO Production

NO is generated continuously by skeletal muscle, a production that is increased by contractions (32, 207). Skeletal muscle normally expresses the neuronal (type I or nNOS) and the endothelial (type III or eNOS) isoforms of NOS. nNOS is strongly expressed in fast-twitch muscle fibers and localized to the muscle sarcolemma where it is associated with the dystrophin-glycoprotein complex (DGC). eNOS is localized to the muscle mitochondria (208). iNOS (type II) is also expressed in skeletal muscle in some inflammatory conditions, but it does not play a significant role in normal muscle (373). Analysis of myotubes in culture has confirmed that skeletal muscle cells per se release increased amounts of NO during contractile activity (292), a release that was greatly reduced by the NOS inhibitor L-NAME. nNOS appears to be the prime source of the NO released from skeletal muscle (156). Passive stretching of muscle has also been shown to increase NO release from rat skeletal muscle in vitro (388) and to increase nNOS expression‎.

C. Generation of ROS in Muscle Following Damage

Nonmuscle sources may play a major role in modifying muscle redox state where tissue damage has occurred, notably through the role played by phagocytic white cells. Substantial injury to muscle fibers is accompanied by invasion of the area with macrophages and other phagocytic cells (e.g., see Ref. 254), and although this process appears to be essential for preparation of the tissue to allow effective regeneration to occur, it also involves the release of substantial amounts of ROS from the phagocytic cells (242). The magnitude of this release can be such that damage to previously undamaged muscle cells may result (417).

VII. RADICALS AND MUSCULAR FATIGUE

In the preceding section we discussed potential sources of ROS and RNS in skeletal muscle and highlighted the fact that skeletal muscles continuously produce ROS and RNS. This segment will address the influence of both RNS and ROS on skeletal muscle force production. Because of the voluminous literature on this topic, it is necessary to condense the material presented in this section. Readers seeking more details should consult the following comprehensive reviews (11, 67, 245, 316–318, 320, 368, 373, 381, 383).

A. Influence of NO on Force Production in Unfatigued Muscle

As discussed previously, NO is produced in mammalian cells via the enzymatic action of a family of NO synthases that are differentially expressed across cell types (207, 208, 336). Furthermore, it is well established that isolated skeletal muscle fibers produce low levels of NO during resting conditions but NO production increases during periods of contractions (207, 310, 319) and that endogenous production of NO can modulate skeletal muscle force production. Indeed, studies using excised bundles of muscle fibers reveal that force production during submaximal tetanic contractions is depressed by NO donors (7, 18, 208, 294, 330) and increased by NOS inhibitors and NO scavengers (18, 207). Interestingly, NO production does not influence maximal tetanic force production. Hence, endogenous NO production shifts the force-frequency curve to the right without decreasing maximal tetanic force production. Furthermore, because NOS3 deficiency does not alter muscle function, it appears that the impact of NO on skeletal muscle contractile properties is likely mediated by NOS1 (156).

B. ROS Modulation of Force Production in Unfatigued Muscle

Similar to NO and muscle force production, it is also established that ROS have an important influence on force production in unfatigued skeletal muscle. For example, the low levels of ROS present in skeletal muscle during basal conditions are a requirement for normal force production (317, 318, 383). Indeed, antioxidant-mediated depletion of ROS from unfatigued skeletal muscle results in a depression of muscle force production (73, 322–324). In contrast, a modest increase in ROS in skeletal muscle fibers results in an increase in force production (322). The positive impact of ROS on muscle force production is reversed at higher ROS concentrations as force production decreases in both a time- and dose-dependent manner (322).

Although numerous investigators have contributed to our understanding of the influence of ROS on muscle force production, Reid et al. (322) were the first to develop a theoretical model to describe the relationship between muscle redox balance and isometric force production. This model is illustrated inFigure 5. Their model assumes that the muscle redox state is a physiologically regulated variable that is balanced by matching the rates of ROS production with cellular antioxidant buffering capacity. The paradigm of Reid et al. (317, 322) predicts that an optimal cellular redox state exists whereby conditions are ideal for muscle force production. It follows that a deviation from the optimal redox balance leads to a loss of force production.

View larger version (17K):
In this window
In a new window

 

FIG. 5. A theoretical model proposed by Reid et al. (322) that describes the biphasic effect of ROS on skeletal muscle force production. Point 1 represents the force production by unfatigued muscle exposed to antioxidants or a reducing agent. Point 2 illustrates the force generated by muscle in its basal state (i.e., no antioxidants or oxidants added). Point 3 illustrates the force produced by unfatigued skeletal muscle exposed to low levels of oxidants; this represents the optimal redox state for force production. Point 4 illustrates the deleterious effects of excessive ROS on skeletal muscle force. [Redrawn from Reid (317).]

C. Radicals Contribute to Exercise-Induced Muscular Fatigue

As outlined in previous sections, redox disturbances in skeletal muscle can significantly reduce force production. This observation has stimulated much interest in the possibility that free radicals contribute to muscular fatigue during prolonged exercise. Indeed, the role of oxidants in muscular fatigue has been investigated using a variety of animal models via in vitro and in situ muscle preparations as well as during whole body exercise. Moreover, studies have explored the role of antioxidant supplementation in preventing muscular fatigue during prolonged exercise in humans. In this segment, we summarize the evidence indicating that free radicals contribute to muscular fatigue. For the purpose of this discussion, we will define muscular fatigue as "an exercise-induced reduction in muscle force generation" (120, 404).

1. Role of redox disturbances in muscle fatigue: in vitro and in situ animal studies

As discussed throughout in this review, there is abundant evidence that production of ROS increases in contracting skeletal muscle. Moreover, animal studies provide convincing evidence that ROS contribute to muscle fatigue induced by prolonged muscular contractions. The earliest report indicating that ROS contribute to muscle fatigue appeared in 1990. This early study demonstrated that the ROS scavenger N-acetylcysteine delayed muscle fatigue in an in situ diaphragm muscle preparation (361). Since this initial report, numerous studies using both in vitro and in situ preparations have investigated the role that redox disturbances play in the development of muscular fatigue. Although one study using dietary antioxidants to scavenge ROS failed to demonstrate a role for ROS in the development of muscle fatigue (73), the vast majority of studies have reported that scavenging ROS via enzymatic and nonenzymatic antioxidants delays muscle fatigue during submaximal contractions (22, 34, 203, 267, 321, 322, 384). In contrast, it appears that antioxidant scavengers do not appear to be effective in delaying fatigue when muscle contractions are near maximum (250, 321). Finally, studies examining the effects of antioxidants on muscle performance during recovery from fatiguing exercise are inconsistent with one report indicating a faster recovery of force production (95), whereas others fail to observe a faster recovery time (203, 321, 325).

In contrast to the literature regarding the impact of ROS on muscle fatigue, evidence that NO production directly contributes to the development of muscle fatigue is limited. In theory, because of the effects of NO on muscle proteins involved in force production (i.e., contractile and calcium handling proteins), it could be predicted that NO should contribute to muscle fatigue (368). Nonetheless, a consensus of literature does not exist to support the notion that NO production promotes muscular fatigue. For example, although one report suggests that NOS inhibition does delay fatigue in electrically stimulated diaphragm muscle (118), other studies conclude that NO does not directly influence skeletal muscle fatigue resulting from prolonged submaximal contractions (65, 207). Furthermore, in perfused in situ muscle preparations, NOS blockade appears to accelerate muscle fatigue due to a dysregulation of blood flow (7, 13). Collectively, these data do not support the concept that muscle-derived NO promotes skeletal muscle fatigue.

2. Radicals and muscle fatigue: in vivo studies

Numerous reports document the failure of supplementation with antioxidant vitamins to improve endurance exercise performance in humans (28, 59, 119,302, 333, 370). Collectively, these results lead to the conclusion that common dietary antioxidants such as vitamin E and C do not improve human exercise performance (68, 196, 302). Moreover, some authors have interpreted these experiments as evidence that ROS do not contribute to muscular fatigue during submaximal exercise in humans. This is not the case as numerous mechanistic studies using a variety of pharmacological antioxidants have clearly demonstrated that exercise-induced production of ROS in skeletal muscle contributes to muscle fatigue. For example, an early animal study using both spin traps and vitamin E demonstrated that scavenging ROS in muscle during exercise delays the onset of muscular fatigue (279). Moreover, a growing number of reports indicate that administration of the antioxidant N-acetylcysteine (NAC) delays muscle fatigue during submaximal exercise in humans (250, 257,260, 261, 326, 391). As an antioxidant, NAC acts as a reduced thiol donor that supports glutathione resynthesis and may exhibit other antioxidant properties (91, 199). Table 3 illustrates some of the published reports indicating that NAC administration delays muscular fatigue during a variety of submaximal exercise tasks including electrically stimulated fatigue of human limb muscle (326), breathing against an inspiratory load (391), cycling exercise (257, 260, 261), and repetitive handgrip exercise (250). The improvements in exercise performance in these experiments ranged from 15 to 50% (Table 3). Indistinguishable from these positive findings in humans, NAC administration has also been shown to delay fatigue in animal experiments using both in vitro and in situ muscle preparations (94, 203, 361, 384). Nonetheless, NAC does not appear to retard muscle fatigue during exercise at near-maximal intensities (94, 250, 259).

View this table:
In this window
In a new window

 

TABLE 3. A list of selected human studies indicating that N-acetylcysteine delays muscular fatigue during prolonged submaximal exercise

VIII. REDOX-SENSITIVE SITES IN SKELETAL MUSCLE

The previous section highlighted the effects of ROS and NO on muscle force production and fatigue. Although it is widely stated that free radicals can damage lipids, proteins, and DNA, overt oxidant-induced damage to skeletal muscle caused by ROS or RNS appears to be relatively rare, but the functions and actions of free radicals, ROS, and RNS are more usually associated with oxidation of critical, redox-sensitive sites within skeletal muscle. This section will describe these sites commencing with those that appear to be important in the actions of ROS and NO in modifying force generation in skeletal muscle. This will be followed by an outline of the manner in which ROS and NO modify cellular signaling pathways and activate proteolytic systems that affect muscle maintenance of muscle mass.

A. Redox-Sensitive Targets in Skeletal Muscle That Impact Force Production

There are various potential mechanisms by which NO and ROS might influence skeletal muscle force production. Figure 6 illustrates putative molecular targets and processes involved in skeletal muscle contraction that are influenced by NO and/or ROS. In addition to affecting calcium regulation, and/or myofilament function, NO might theoretically impact on force production through lowering ATP production. The NOS3 isoform is closely associated with skeletal muscle mitochondria, and NO production from this site could impair muscle force production by limiting mitochondrial ATP production via inhibition of cytochrome oxidase (56, 58) or other mitochondrial enzymes (47). NO derivatives also inhibit the activity of both glyceraldehyde-3-phosphate dehydrogenase (265, 266) and creatine kinase (131) that could limit ATP production. Nonetheless, there is currently no evidence that NO limits ATP production in muscle during repetitive contractions.

View larger version (33K):
In this window
In a new window

 

FIG. 6. Illustration of putative redox-sensitive targets in skeletal muscle that can influence force production. SOD, superoxide dismutase; NOS, nitric oxide synthase; NO, nitric oxide; SERCA, sarcoplasmic reticulum calcium ATPase. [Redrawn from Smith and Reid (368).]

A considerable amount of work has examined whether NO acts to influence force production through its usual second messenger, cGMP, or through non-cGMP-dependent processes. Both muscle contractions and NO donors increase cGMP concentration in skeletal muscle, and inhibition of NOS depresses cGMP levels (226). Immunohistochemical studies reveal that cGMP content is not fiber type specific and that cGMP is located near the subsarcolemma and in close proximity to NOS1 (286). Although it is not universally accepted (35, 50), several authors argue that interventions that increase cGMP signaling (e.g., NO donors, cGMP analogs, etc.) reduce skeletal muscle force production (1, 38, 207, 244, 245, 319). In contrast, contractile force is increased by treatments that decrease cGMP levels (e.g., NOS inhibitors and guanylate cyclase inhibitors) (207). Nonetheless, the magnitude of the cGMP-dependent changes in muscle contraction is consistently smaller than the aggregate effect of NO on muscle force production (318). Specifically, it appears that ∼50% of the NO-induced depression in muscle force production is mediated via cGMP action (317). Hence, NO-induced depression of muscle force production occurs via both a cGMP-dependent and cGMP-independent mechanism (319).

Collectively, the current data suggest that the predominant effects of ROS and NO on muscle force production are due to changes in calcium handling by muscle cells or due to a change in the sensitivity of myofilaments to calcium. A discussion of the redox influence on SR calcium handling and redox modifications to myofibrillar proteins follows.

1. Redox influence on SR calcium regulation

The redox influence on SR function has been studied extensively in both cardiac and skeletal muscle (2, 3, 21, 247, 340, 409, 415, 424). The ryanodine receptor (RyR) calcium release channel in the SR is a large homotetramer composed of 565-kDa subunits; each subunit contains sulfhydryl groups that are sensitive to redox modulation (281, 380, 418, 425) and is activated by both exposure to ROS and by changes in the environmental redox status of key cellular thiols such as glutathione (424). In situ, the RyR appears to be in close association with the NADP(H) oxidase(s) found in the SR, and locally generated superoxide appears to be the major ROS capable of influencing this channel (409).

Exposure of skeletal muscle fibers and isolated SR proteins to exogenous ROS reveals that regulatory proteins of the SR calcium release channels are oxidized (2, 21, 295). In isolated systems, high levels of ROS increase the probability of opening the ryanodine-sensitive calcium release channel, resulting in increased calcium release from the SR (21, 110). In contrast, reducing agents and antioxidants have the opposite effect and retard calcium release from the SR (322). Although ROS exposure has been shown to alter the release of calcium from the SR in isolated systems, research using mechanically skinned muscle fibers suggests that the redox potential across the SR does not influence excitation-contraction coupling in rat muscle (297). Moreover, oxidizing the calcium release channels in the SR does not alter the amount of calcium released during twitch contractions (297). Andrade and colleagues (16, 19) have also investigated this issue using intact single muscle fibers. Their work suggests that exposure of fibers to ROS results in a significant increase in myoplasmic calcium levels in resting (i.e., noncontracting) muscle (16, 19). This increase in resting calcium levels in fibers appeared to reflect a slowing of SR calcium uptake rather than an increase in calcium release from the SR. Similar to the work of Posterino et al., Andrade and co-workers (16, 19) report that exposure of muscle fibers to ROS has a limited impact on SR calcium release during submaximal tetanic contractions. Therefore, based on these collective experiments, it appears that exposure of muscle fibers to ROS does not have a significant impact on myoplasmic calcium transients during both twitch and tetanic muscular contractions.

High levels of ROS also inhibit SR calcium ATPase (SERCA) activity (132, 410), but paradoxically, reductive stress inhibits SERCA function by reducing sulfhydryl groups on SERCA that are required for ATP hydrolysis (83). SERCA1 and SERCA2 proteins contain critical thiol residues and are influenced by both cellular redox status and ROS exposure. ROS inhibit SERCA function by both interfering with the ATP binding site and by uncoupling calcium uptake from ATP hydrolysis (348, 410). There is some variability in the responses of different SERCA isoforms to inhibition by ROS (37).

NO also has significant effects on SR calcium handling, although the specific target of NO/cGMP signaling remains unclear. Inhibition of soluble cGMP in isolated single muscle fibers results in increased cytosolic calcium transients during a tetanic contraction (18). A potential mechanism for the influence of NO on both skeletal and cardiac muscle is through the inhibition of phospholamban, a protein that retards SERCA activity (337, 419, 420). However, phospholamban is expressed only in cardiac and type I skeletal muscle fibers, and therefore, this mechanism cannot explain the NO/cGMP inhibition of muscle force production in fast type II fibers (275). As previously indicated, SERCA contain a small number of sulfhydryl groups that regulate SERCA activity (401, 402), and exposure to high levels of NO inhibits SERCA activity via thiol oxidation (402) and nitration of tyrosine residues (401). Overall, NO-mediated changes in SERCA sulfhydryl groups result in reduced calcium uptake into the SR and increases in cytosolic calcium levels.

NO donors have complex effects of the RyR (153), which shows a biphasic response to NO (341, 373). At low levels, NO prevents oxidative activation of the RyR by acting as an antioxidant (i.e., scavenging superoxide) without directing modifying channel function (142), but in contrast, prolonged exposure to high levels of NO inhibits ryanodine activity and retards calcium release (142, 152).

2. ROS and NO impact on myofibrillar proteins

Although it is clear that ROS and NO can modify SR calcium handling, growing evidence also indicates that ROS can also influence myofilaments structure and function. In this regard, Michael Reid and colleagues have meticulously investigated potential pathways that might be involved in modification of skeletal muscle force generation and have concluded that the most likely explanation lies in ROS influencing the calcium sensitivity of myofilaments (for a review, see Ref. 368). They based this conclusion on observations indicating that 1) high levels of ROS exposure can alter myofilament structure (60, 61, 79, 143), 2) exogenous ROS diminish calcium sensitivity of myofilaments (17, 19), 3) ROS exposure alters cross-bridge kinetics (19), and 4) muscle-derived ROS have been shown to potentiate the increasing calcium insensitivity of fatiguing muscle (267). Several myofilament proteins are oxidized during exposure to ROS, and myofilament function is impaired by prolonged exposure to high levels of ROS (70, 411). For example, myosin heavy chain molecules are targets of ROS, and oxidation of myosin heavy chain proteins promotes an impairment in myosin function (70, 132, 411). Troponin C is also sensitive to ROS-mediated oxidation, and oxidation of troponin C may alter the function of this protein (191, 296).

Studies also indicate that exposure of isolated muscle fibers to NO donors results in impaired myofilament function (150, 294). Several myofilament proteins are candidates to explain this response. Myosin heavy chains contain several sulfhydryl residues that are redox sensitive; however, modification of these thiol groups may not impair function greatly (79). In contrast, actin and tropomyosin do not appear to be redox sensitive (237, 406), and limited information exists regarding the effects of NO on other skeletal muscle contractile proteins.

3. Levels of ROS required to activate redox-sensitive targets

Note that the above discussion extensively uses terms such as "low" or "high" level of ROS, and this reflects the difficulty in designing and eval‎uating studies in this area. Currently, we lack reliable data to quantify ROS within cells, even where these substances are relatively stable such as in the case of hydrogen peroxide. Few studies of the hydrogen peroxide content of body fluids have been undertaken, but microdialysis studies indicate that the skeletal muscle interstitial hydrogen peroxide concentration is on the order of 10–15 μM (395). Nonmuscle cells have been shown to respond to concentrations of hydrogen peroxide of this order (15 μM), with adaptive changes in stress gene expression‎ (202). Muscle cells have been reported to activate adaptive changes in gene expression‎ when exposed to 100 μM extracellular hydrogen peroxide. These authors also reported that muscle cells lose viability when exposed to >1 mM hydrogen peroxide (251, 254). These data contrast with experiments performed with levels of exogenous oxidants that appear excessively high. In this regard, concentrations of hydrogen peroxide of up to 50 mM have been used to mimic the effects of the rise in ROS activities seen following contractions on components of skeletal muscle (308). Antunes and Cadenas (20) have examined the concentration difference across membranes when hydrogen peroxide is used in such situations and calculated that an approximately sevenfold drop occurs across biological membranes, implying that an intracellular concentration of 2–15 μM hydrogen peroxide may be sufficient to induce adaptive changes in gene expression‎ dependent on the cell type under study.

Some studies with these more physiological levels of oxidant have been undertaken with, for instance, Reid and colleagues confirm‎ing effects on the calcium sensitivity of myofibrils (17, 19) and some recent data supporting the hypothesis that more physiological levels of hydrogen peroxide (10 μM) can activate the RyR in situ (282).

B. Cellular Signaling Pathways

ROS can act through several different pathways of signaling transduction including calcium-dependent pathways, protein tyrosine kinases and protein tyrosine phosphatases, serine threonine kinases, and phospholipases (169). According to Allen and Tresini (12), almost half of the ROS effects reported involve members of the mitogen-activated protein kinase (MAP kinase) and nuclear factor κ-B (NFκB) pathways, but effects are not limited to these processes. While it is also generally assumed that these effects are modulated by ROS-induced oxidation, antioxidants can also stimulate some specific gene expression‎ pathways (12). Lander (225) proposed that the cellular responses stimulated by ROS could be classified into the following catergories: modulation of cytokine, growth factor, hormone release and action, ion transport, transcription, neuromodulation, and apoptosis. In contrast, Jackson et al. (169) concluded that the predominant effects of ROS on cells related to increasing or decreasing cell proliferation, changing immune function, and/or induction of apoptotic cell death. Unfortunately, it is currently unknown if ROS influence all of these processes in skeletal muscle as the effects of ROS on contractile properties have been the predominant area of study.

Ji (176) has extensively studied the role of ROS activation of NFκB and MAP kinases in the adaptations of muscle cells to oxidative stress and concluded that these pathways are critical cellular responses to maintain muscle homeostasis through upregulation of the expression‎ of antioxidant enzymes and other cytoprotective proteins. The initial adaptive responses that occur in skeletal muscle following an increase in ROS activity induced by contractions appear to be in the antioxidant defense enzymes and the stress or heat shock proteins (HSPs), and increased muscle content of several of these proteins has been associated with significant protection against subsequent cellular damage (157, 251, 254). A single isometric contraction protocol in mouse muscle, which had been previously shown to increase muscle ROS generation, also increases the activity of muscle antioxidant defense enzymes such as SOD and catalase together with HSP60 and HSP70 content (253), changes which were replicated in studies of human muscle (201). Presupplementation with vitamin C (202) or other antioxidants (168) reduced these adaptive responses, supporting the possibility that these adaptations were regulated by ROS. Complimentary data from Gomez et al. (128) indicate that treatment of rats with antioxidants prevented several exercise-induced changes in skeletal muscle gene expression‎.

NFκB and activator protein-1 (AP-1) appear to be key transcription factors in the upregulation of antioxidant enzymes such as SOD and catalase in response to oxidative stress (169, 176, 422). HSP expression‎ in response to acute stress in eukaryotic cells is primarily regulated by the transcription factor heat shock factor 1 (HSF1; Ref. 76). Studies have shown that the activation of this latter transcription factor is dependent on the increased production of ROS and/or the presence of oxidized or unfolded cellular proteins (114). Furthermore, evidence exists that the increased muscle production of ROS during a period of contractions results in the transient oxidation of protein sulfhydryl groups, and this oxidation is reversible within 60 min following the contraction protocol (253). Activation of NFκB, AP-1, and HSF transcription factors in skeletal muscle has been demonstrated following an isometric contraction protocol that also caused increased ROS generation and resulted in increased content of HSPs, SOD, and catalase (396).

The processes by which ROS regulate these pathways continue to be investigated, but current data suggest that site-specific oxidation of thiol groups play an important role. Cellular thiols are readily oxidized by ROS and, although they have been generally thought to play a role in protection of cells against oxidative damage, this oxidation leads to a change in the thiol-to-disulfide ratios. Site-specific changes in these ratios (also described as the local redox potential) are important in regulation of the activities of some signaling molecules and transcription factors within the cell. Particularly important in this respect is the redox status of GSH and thioredoxin (both TRx1 and TRx2) in different parts of the cell. There is evidence that thiols in different subcellular locations have differential susceptibilities to oxidation and are not in equilibrium. Thus the redox potential differs between subcellular compartments. The GSH and TRx content of mitochondria and nuclei appear to be regulated independently of cytosolic contents with, for instance, a small pool of GSH within nuclei that does not exchange freely with cytosolic GSH (39, 174, 366). Assessment of the redox potential of GSH/GSSG within organelles is complex, but a modification of western blotting allows quantification of reduced and oxidized TRx in subcellular fractions (124). TRx1 is found within the cytoplasm and nuclei of cells and TRx2 within mitochondria (124). The redox potential of the different pools of thiols have been calculated for nonmuscle cells and show that mitochondrial TRx2 is the most reduced (approximate value –340 mV), nuclear TRx1 is intermediate (approximately –300 mV), and cytosolic TRx1 is most oxidized (approximately –280 mV) (403). For GSH/GSSG, the mitochondrial redox potential has been calculated to be approximately –280 mV and the cytosolic potential to be –260 to –200 mV (141).

A detailed discussion of redox control of gene expression‎ is beyond the scope of this review, and therefore, only a brief summary will be outlined here. For detailed reviews of this topic, the reader is referred to recent reviews focused on this topic (103, 176, 220, 238, 243, 278). Many transcription factors are redox sensitive, but NFκB has been cited as a typical redox-regulated factor and ROS have been claimed to be principal regulators of NFκB activation in many situations (268). NFκB exists in the cytoplasm as an inactive heterodimer when bound to an inhibitory subunit IκB. When activated by dissociation of IκB, NFκB migrates to the nucleus and binds to NFκB binding sites in the promoter sequences of a variety of genes. Activation by ROS appears to involve oxidation of key cysteine residues in the upstream activators of NFκB and in many situations is prevented by antioxidants or reducing agents (141). A further level of redox control is also evident within the nucleus where binding of the NFκB to DNA requires a reducing environment (187). Analogous redox control occurs for AP-1 and other transcription factors where ROS-induced oxidation stimulates cytosolic activation, but a reducing environment is required for nuclear DNA binding and activation of transcription (169).

C. Oxidants and Nonspecific Oxidative Damage to Lipids, Proteins, and DNA

Recent developments have questioned the validity of the original concept of oxidative stress, particularly due to the realization that individual signaling and control events occur through discrete redox pathways rather than through mechanisms that are directly responsive to a change in the global thiol/disulfide balance (186). Hence, measurements of individual ROS and subcellular oxidation targets may be more important than global markers of oxidative damage, but nevertheless there are numerous reports that increased ROS activity during exercise leads to increases in oxidation of markers of lipid peroxidation, protein oxidation, and DNA oxidation (see Ref. 354 for comprehensive reviews). The relationship of increases in these markers of oxidative damage to redox-modulated physiological responses of muscle to exercise is unclear as is the desirability of attempting to suppress these changes through the use of antioxidants. A schematic diagram illustrating the conceptual relationship between markers of oxidation and physiological responses is shown in Figure 7.

View larger version (14K):
In this window
In a new window

 

FIG. 7. Hypothetical model depicting muscle responses to increasing oxidation of redox-sensitive components due to increased ROS exposure. It is envisaged that a low-level increase in ROS exposure changes the cellular redox state to initially affect redox-sensitive signaling pathways and if sufficiently large or sustained may lead to cellular damage (middle panel). It seems likely that it will not be possible to clearly demarcate the amounts of oxidation required to invoke differing responses, and hence, the boundaries between these actions are not shown as solid lines. Where in this spectrum of events traditional marker of ROS activity, such as measures of lipid, DNA, or protein oxidation, become abnormal is currently unknown (and hence these are also demarcated by dotted lines in left panel). Contractile activity is known to lead to an increase in ROS generation in muscle, but the factors that govern when this is sufficient to change the redox status and activate redox-sensitive signaling event or lead to damage are not understood (right panel). Possible factors influencing the magnitude of this response include the nature and duration of the contractile activity, the antioxidant status of the muscle, and the basal redox status prior to exercise.

D. ROS-Mediated Activation of Calpains and Caspases

Growing evidence suggests that ROS species can serve as second messengers in cellular signal transduction pathways that promote proteolysis in skeletal muscle during periods of inactivity. The first evidence that oxidants played a signaling role in the regulation of disuse muscle atrophy was provided by Kondo et al. (212). The pioneering work of Kondo and colleagues (211–215) revealed that immobilization of skeletal muscles is associated with increased ROS production resulting in oxidative injury in inactive muscle fibers and that disuse muscle atrophy could be retarded via antioxidants. These early observations have subsequently been confirmed by others (23, 42, 249), and it is now clear that oxidative stress can contribute to the rate of disuse muscle atrophy by promoting the activation of calpain and/or caspase-3 (reviewed in Refs. 305, 306). A brief summary of the redox regulation of each of these proteases follows.

1. ROS and calpain activation

Calpains are Ca²⁺-dependent cysteine proteases found in all mammalian cells (127). Numerous members of the calpain family exist, but the two best characterized calpains are labeled as μ-calpain and m-calpain; these terms refer to the micromolar and millimolar amounts of calcium required to activate each respective calpain isoform (127). Activation of calpain results in a release of sarcomeric proteins via the cleavage of cytoskeletal proteins (e.g., titin, nebulin) that anchor contractile elements (210, 309).

In vivo calpain activity is regulated by multiple factors including cytosolic free calcium levels and the concentration of the endogenous calpain inhibitor calpastatin (127). In this regard, calpain activity can be elevated by a sustained increase in free calcium in the cytosol and/or a decrease in calpastatin levels (127). In reference to calpain during disuse muscle atrophy, it is clear that skeletal muscle inactivity promotes an increase in both cytosolic calcium levels and calpain activation (214, 241, 357, 379, 387). Although the mechanism responsible for this inactivity-mediated calcium overload is unknown, it is feasible that intracellular production of ROS plays a key role in calcium dyshomeostasis. A potential mechanism to link oxidative stress with calcium overload is that ROS-induced formation of reactive aldehydes (i.e., 4-hydroxy-2,3-trans-nonenal) have been reported to inhibit plasma membrane Ca²⁺-ATPase activity (362). Hence, an oxidative stress-induced decrease in Ca²⁺-ATPase activity would hinder Ca²⁺ removal from the cell and encourage intracellular Ca²⁺ accumulation. However, it is currently unknown as to whether this mechanism is the solitary explanation for inactivity-mediated calcium overload in muscle as numerous other possibilities exist (218).

2. ROS regulation of caspase-3 activity

Accumulating evidence indicates that caspase-3 contributes to protein degradation and muscle atrophy (104, 255). More specifically, caspase-3 activation promotes degradation of actomyosin complexes, and inhibition of caspase-3 activity suppresses the overall rate of skeletal muscle proteolysis in diabetes-mediated cachexia and diaphragmatic atrophy following prolonged periods of mechanical ventilation (104, 255).

Control of caspase-3 activity in the cell is complex and involves numerous interconnected signaling pathways. In the case of diabetes-induced muscle atrophy, it seems possible that caspase-3 is activated by caspase-12 (via a calcium release pathway) and/or activation of caspase-9 (via a mitochondrial pathway) (104). A potential interaction between these caspase-3 activation pathways is that both of these can be activated by ROS (306).

IX. SUMMARY AND FUTURE DIRECTIONS

The first evidence that muscular exercise promotes oxidative damage in tissues appeared in 1978. Since this first report, the field of redox biology has evolved significantly, and our understanding of the sources and consequences of exercise-induced free radical production has advanced markedly. Current evidence suggests that contracting muscles produce oxidants from a variety of cellular locations. Furthermore, although mitochondria are a potential source of ROS in cells, growing evidence suggests that these organelles may play a less prominent role in oxidant production in contracting skeletal muscles than was previously thought.

Many early studies investigating exercise and free radical production focused on the damaging effects of oxidants in muscle (e.g., lipid peroxidation). However, a new era in redox biology exists today with an ever-growing number of reports detailing the advantageous biological effects of free radicals. Indeed, it is now clear that ROS and RNS are involved in modulation of cell signaling pathways and the control of numerous redox-sensitive transcription factors. Furthermore, physiological levels of ROS are essential for optimal force production in skeletal muscle. Nonetheless, high levels of ROS promote skeletal muscle contractile dysfunction resulting in muscle fatigue.

While the field of redox biology has advanced rapidly during the past two decades, many questions remain unanswered. Therefore, in an effort to simulate additional research in this field, the remainder of this section will focus on the identification of important redox topics that warrant additional work.

First, we believe that the lack of any true quantitative measures to assess the redox status of muscle cells and organelles is a considerable hindrance to investigators and is an important area where developments are required. Moreover, the development of sensitive and reliable techniques to assess the redox status of individual proteins will be required for scientists to identify specific factors responsible for redox-regulating processes in the cell.

Another technical limitation in the field of redox biology is the lack of tools available to accurately measure (and hence compare) the production of specific radicals in living cells or organelles in vivo. That is, at present, no techniques exist to quantify the specific rate of radical production in cell compartments. While it has been possible for the field to develop thus far using indirect measures of ROS activity, a full eval‎uation of the generation of specific ROS and redox-regulated responses will only be achieved with the development of new analytical approaches. Current approaches limit research progress in many areas of redox biology including the study of radical production in contracting skeletal muscle fibers. Some new developments in this area offer promise including novel probes for specific ROS, such as HyPer, a genetically encoded probe that can be transfected into mammalian cells and in which yellow fluorescent protein is inserted into the regulatory domain of the prokaryotic hydrogen peroxide sensing protein OxyR (40). This probe may be specifically targeted to organelles, such as mitochondria. A complementary approach in which a common ROS probe, dihydroethidium, has been linked through a hexyl carbon chain to a triphenylphosphonium group to produce a compound (known as MitoSOX or Mito-HE) that accumulates in mitochondria also holds considerable promise (332). New methods are evolving to measure the oxidation of specific thiols in various cellular compartments. With the use of these tools, emerging evidence suggests that changes in redox status within cells may be compartmentalized and that this may serve as a mechanism for regulating specific redox signaling pathways within cells. For example, it is feasible that both mitochondrial and nuclear compartmentalization of redox signaling exists, and this could be important in exercise-induced changes in redox signaling in muscle fibers (140). This is a critical topic for future work because improving our understanding of redox compartmentalization in cells may provide key insight into redox signaling in muscle fibers. In a related area, improving our understanding of the nature of redox-sensitive targets in skeletal muscle is important. It seems highly unlikely that the various potential targets in cells show an equivalent sensitivity to specific ROS and thus there is likely to be a hierarchy for oxidation and functional change that is dependent on factors such as their location compared with the site of generation of the radical species, the nature of the oxidizing species, the local concentration of antioxidants, etc. Knowledge of these processes would undoubtedly aid future growth in this field.

Another important area for further work, which arises due to the increasing use of cell culture and isolated tissue models over the last 25 years, is the effect of the in vitro environment on ROS generation and responses in cells and tissues. This has not been widely studied, but cell culture models are generally undertaken at ∼20% environmental oxygen, and isolated muscle tissue models use protocols that involve predominantly bubbling of bathing solutions with 95% oxygen-5% carbon dioxide, although the oxygen environment of muscle cells at rest in vivo is much less oxygen rich and appears to be between 2 and 10% (81, 367). Although limited studies exist, Csete and colleagues (80, 81, 270) propose that high oxygen tensions modify muscle cell fate and phenotype through processes that involve increased ROS generation in the oxygen-rich environments. Clearly, this is an important issue that warrants additional research to determine if muscle cell lines that are maintained at high oxygen tensions develop phenotypes that influence experimental outcomes.

The development of targeted antioxidants remains an important area of research in redox biology. Indeed, the development of a highly effective and targeted antioxidant could provide researchers with a sensitive antioxidant probe to explore mechanistic questions in this field. For example, using a mitochondrial targeted antioxidant, researchers could study specific mitochondrial ROS production and its consequences using organelle specific antioxidants (5, 57, 274, 369).

Increased use of genetically modified experimental animals (e.g., conditional and tissue specific knockout and overexpressers) could prove to be a valuable tool to scientists working on redox-related problems. Also, the use of gene silencing tools (e.g., siRNA) in studies of redox biology provides a unique opportunity for investigators to study the impact of a specific gene product in cells. Allied to this area is the use of experimental animals or cells that are genetically modified to provide a technique to monitor the activity of a specific molecular process in vivo or in vitro. The best known of these are the NFκB reporter mice that are modified to express luciferase when NFκB is activated, such as occurs following ROS exposure. Models such as this provide a new opportunity to study key signaling processes in vivo. This approach has already been utilized to examine the effect of antioxidants on NFκB activation in vivo (271), but the relevance to exercise does not appear to

[권강범]

내 생각엔 활성산소가 스트레스 발생시(운동포함) 발생되는데 분명히 활성산소를 제거해 주는 기전이 더 활성화될걸로 생각됨. 활성산소에 대한 개괄적인 설명을 쌍둥이 논문 이후에 한번 하겠습니다. 많은 사람이 활성산소에 대해 오해를 하고 있는데 이 활성산소를 문제로 여기는 것 자체가 문제인 걸로 생각됨. 인체의 항산화 기전이 나이가 듦에 따라 약해져서 노화가 진행되는데 이를 어찌 약물로 막을수 있을까요? 이는 자연의 순리....ㅋㅋ

[문형철]

우선 과도한 운동, 과도한 스트레스반응은 활성산소의 발생을 많이하게하고, 인체는 활성산소를 제거하는 기전이 존재하는데, 이 과정에서 적절한 미네랄, 비타민이 필요. 그래서 항산화제로 비타민이 뜨는거고..

[문형철]

aging은 인체의 활동속에서 발생하는 활성산소의 처리능력이 저하되는 과정이고.. 의료인은 어떻게 하면 활성산소의 처리능력을 젊은 상태로 유지할것이냐? 이게 질문의 초점이다. ㅎㅎㅎ

[정기홍]

내가 전에 정리했던 노화에 대한 내용은 대략 이렇다. 노화를 설명하는 이론은 첫째, 손상설. 그러니까 생명에 필수적인 요소들에 대한 손상이 쌓여가면서 노화가 진행된다는 것. 산화 반응, 갈색화 반응, 자유라디칼(활성산소) 발생이 그러한 손상의 유형이고, 이런 손상을 줄이거나 지연시키는 것으로 어느 정도 효과가 인정된 것으론 절식(적절한 영양공급에 기반한 소식)이 있다. 절식을 꾸준히 하면 평균 2~3년 정도의 수명 연장 효과가 있는 것으로 보인다나? 적절한 운동 역시 도움이 되지만, 운동은 노화 자체, 그러니까 수명 연장에 기여한다기보다는 건강에 기여하는 면이 더 크다고 보는 게 현재로선 더 적절한 판단인 듯.

[정기홍]

둘째, 프로그램설. 노화는 태어날 때부터 유전자에 프로그램되어 있다는 이론이라고 한다. 텔로미어와 텔로머라제로 설명되는. 텔로미어와 텔로머라제는 강범이가 자세히 알테니 언제 기회되면 자세한 소개 부탁한다. 내가 아는 바로는 세포가 복제될 수록 텔로미어가 점점 짧아지고, 결국 한계에 도달하면 세포는 사멸한다는 것. 헤이플릭 한계로 알려진 세포분열의 횟수는 대략 50회 정도인데 그만큼의 분열을 한 세포는 더 이상 분열을 못하고 늙고 사멸한다는 내용인 듯. 그러니까 우리 세포는 태어날 때부터 죽도록 이미 프로그램되어 있다는 게 이 이론의 골자고, 노화를 조절하기 위해선 텔로머라제를 이용하여 텔로미어가 줄어들지

[정기홍]

않게 조절할 수 있는 기전을 연구하는 방향이 있다고 들었다. 언제 기회되면 관련 내용도 한번 읊어주면 무지 고맙겠다. ^^

위에서 말한 노화에 대한 두 가지 이론은 근접적 설명, 즉 노화가 일어나는 기전에 대한 설명이고 보다 궁극적인 설명, 그러니까 인간에게 생명체에게 왜 노화가 있는가에 대한 보다 근본적인 설명은 못하고 있는데, 그런 궁극적인 설명은 진화론적인 시각으로 이해하면 금방 수긍할 수 있다. 말로 전달하면 몇 분 안 걸릴 내용인데.. 간단히 말하면 생명체의 번식기 이전의 삶은 질병과 노화로부터 저항할 수 있도록 진화했지만 번식 이후의 삶은 그렇지 못하다는 것. 그래서 번식기를 거쳐 늙고 병드는 건

[정기홍]

그래서 노화와 죽음을 맞이하는 건 자연의 법칙상 어쩔 수 없는, 아니 오히려 당연한 결과라는 것. 자세한 내막을 들여다보면 빈틈이 느껴지지 않을 만큼 매우 논리적인 설명임을 알 수 있는데, 단순화시켜 표현하면 우리가 늙고 죽는 것은 섹스, 즉 번식을 하는 댓가라고 말할 수 있다. 그리고 지금까지의 연구에 의하면 수명 자체는 후천적 노력보다 유전에 의한 영향이 더 크다는 것 같더라. 노화에 대한 전반적인 내용을 이해하고 난 뒤 여러 생각이 오갔는데 그 중 하나가 이거다. 삶 자체가 정말 기적 중의 기적이라는 것, 늙지 않고, 죽지 않기를 바라는 게 얼마나 말도 안되는 욕망인지를 더 깊이 이해하게 됐다는 것.

[정기홍]

그러면서 나에게 허락된 만큼의 삶을 충실하게, 건강하게 행복하게 살아가야겠다는, 그렇게 하기 위해 나를 잘 다스리며 살아가야겠다는 다짐이 저절로 생기더라. 그러면서 삶에 대한 의욕이 더욱 샘솟는 걸 느꼈다. 즐거운 주말 보내라~!!

[문형철]

ㅎㅎㅎ 고마워 .. 의학과 접목할 지식이 필요한데...

[여인호]

읽기