응화생 화학생명공학실험 II & III

※ 결과 <table class="txc-table" width="700" cellspacing="0" cellpadding="0" border="0" style="line-height: 19.2px; border-collapse: collapse; border: none;"><tbody><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td colspan="1" rowspan="2" style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 50px; border: 1px solid rgb(204, 204, 204);">Glucose 농도 (S, mM) </td><td colspan="2" rowspan="1" style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 466px; height: 25px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-top-width: 1px; border-top-style: solid; border-top-color: rgb(204, 204, 204);">효소 반응 속도 (V, nA)</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">1조</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">2조</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204);">0</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">94.44</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">83.652</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204);">3.125</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">304</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">169.496</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204);">6.25</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">524.9</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">274.811</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204);">12.5</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">973.9</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">380.611</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204);">25</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">1284</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">519.154</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204);">50</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">1612</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">633.603</td></tr><tr style="font-family: 굴림, gulim, tahoma, sans-serif; line-height: 1.6;"><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204);">100</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">1767</td><td style="font-family: 굴림, gulim, tahoma, sans-serif; font-size: 12px; width: 233px; height: 24px; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-width: 1px; border-right-style: solid; border-right-color: rgb(204, 204, 204);">692.294</td></tr></tbody></table> 위의 데이터와 protocol 2페이지에 있는 표를 참고해서 enzyme kinetics 방정식 세 종류의 그래프를 그리고 각각의 Km, Vmax 값을 구하시면 됩니다. 그리고 각각의 Km, Vmax 값을 서로 비교해보고 어떤 차이점이 발생하는지, 왜 차이가 나타나는지에 대한 본인의 생각을 고찰에 써보세요. 결과보고서 양식입니다. 1. 표지 : 일자/요일반/학번/이름/조2. 실험목적3. 실험과정4. 결과5. 고찰6. 참고문헌 ※ 예비 예비보고서 양식은 지난주와 동일하며 포함되어야하는 내용은 아래와 같습니다. 1. Enzyme immobilization method2. Dendrimer3. 3전극계4. Cyclic voltammetry5. Glucose detection method 기타 질문사항은 010-9904-4396 (임성현)으로 21시 전까지 연락주시면 됩니다.